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Title: A comparative genomics study of 23 Aspergillus species from section Flavi

Journal Article · · Nature Communications
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  1. Technical Univ. of Denmark, Kongens Lyngby (Denmark)
  2. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  3. Kikkoman Corporation, Noda (Japan)
  4. Utrecht Univ. (Netherlands)
  5. Univ. of Helsinki (Finland)
  6. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
  7. Aix-Marseille Univ., Marseille (France)
  8. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
  9. Kanazawa Inst. of Technology, Ishikawa-ken (Japan)
  10. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Section Flavi encompasses both harmful and beneficial Aspergillus species, such as Aspergillus oryzae, used in food fermentation and enzyme production, and Aspergillus flavus, food spoiler and mycotoxin producer. Here, we sequence 19 genomes spanning section Flavi and compare 31 fungal genomes including 23 Flavi species. We reassess their phylogenetic relationships and show that the closest relative of A. oryzae is not A. flavus, but A. minisclerotigenes or A. aflatoxiformans and identify high genome diversity, especially in sub-telomeric regions. We predict abundant CAZymes (598 per species) and prolific secondary metabolite gene clusters (73 per species) in section Flavi. However, the observed phenotypes (growth characteristics, polysaccharide degradation) do not necessarily correlate with inferences made from the predicted CAZyme content. Our work, including genomic analyses, phenotypic assays, and identification of secondary metabolites, highlights the genetic and metabolic diversity within section Flavi.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)
Sponsoring Organization:
Villium Foundation; Danish National Research Foundation; Villum Foundation; Jorck Foundation; USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231; VKR023437; DNRF137; AC05-76RL01830
OSTI ID:
1619580
Alternate ID(s):
OSTI ID: 1605144; OSTI ID: 1619163
Report Number(s):
PNNL-SA-146998; ark:/13030/qt3mr4c31c
Journal Information:
Nature Communications, Vol. 11, Issue 1; ISSN 2041-1723
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 79 works
Citation information provided by
Web of Science

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