Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide
Abstract
Abstract Biofilms are matrix-associated communities that enable bacteria to colonise environments unsuitable for free-living bacteria. The facultative intracellular pathogen Francisella tularensis can persist in water, amoebae, and arthropods, as well as within mammalian macrophages. F . tularensis Types A and B form poor biofilms, but F . tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells. The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex. However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan. In addition, the surface polysaccharide composition of the bacterium affected the protein:DNA:polysaccharide composition of the biofilm matrix. In contrast, F . novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F . novicida biofilm formation. These results indicated that suppression of surface polysaccharides may promote biofilm formation by F . tularensis Types A and B. Whether biofilm formation enhances survival of F . tularensis in aquaticmore »
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1619576
- Alternate Identifier(s):
- OSTI ID: 1610216
- Grant/Contract Number:
- FG02-93ER20097
- Resource Type:
- Published Article
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Name: Scientific Reports Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics
Citation Formats
Champion, Anna E., Catanzaro, Kelly C. Freudenberger, Bandara, Aloka B., and Inzana, Thomas J. Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide. United Kingdom: N. p., 2019.
Web. doi:10.1038/s41598-019-48697-x.
Champion, Anna E., Catanzaro, Kelly C. Freudenberger, Bandara, Aloka B., & Inzana, Thomas J. Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide. United Kingdom. https://doi.org/10.1038/s41598-019-48697-x
Champion, Anna E., Catanzaro, Kelly C. Freudenberger, Bandara, Aloka B., and Inzana, Thomas J. Thu .
"Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide". United Kingdom. https://doi.org/10.1038/s41598-019-48697-x.
@article{osti_1619576,
title = {Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide},
author = {Champion, Anna E. and Catanzaro, Kelly C. Freudenberger and Bandara, Aloka B. and Inzana, Thomas J.},
abstractNote = {Abstract Biofilms are matrix-associated communities that enable bacteria to colonise environments unsuitable for free-living bacteria. The facultative intracellular pathogen Francisella tularensis can persist in water, amoebae, and arthropods, as well as within mammalian macrophages. F . tularensis Types A and B form poor biofilms, but F . tularensis mutants lacking lipopolysaccharide O-antigen, O-antigen capsule, and capsule-like complex formed up to 15-fold more biofilm than fully glycosylated cells. The Type B live vaccine strain was also 50% less capable of initiating surface attachment than mutants deficient in O-antigen and capsule-like complex. However, the growth medium of all strains tested also influenced the formation of biofilm, which contained a novel exopolysaccharide consisting of an amylose-like glucan. In addition, the surface polysaccharide composition of the bacterium affected the protein:DNA:polysaccharide composition of the biofilm matrix. In contrast, F . novicida attached to surfaces more efficiently and made a more robust biofilm than Type A or B strains, but loss of O-antigen or capsule-like complex did not significantly affect F . novicida biofilm formation. These results indicated that suppression of surface polysaccharides may promote biofilm formation by F . tularensis Types A and B. Whether biofilm formation enhances survival of F . tularensis in aquatic or other environmental niches has yet to be determined.},
doi = {10.1038/s41598-019-48697-x},
journal = {Scientific Reports},
number = 1,
volume = 9,
place = {United Kingdom},
year = {2019},
month = {8}
}
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