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Title: Efficacy of dalbavancin against MRSA biofilms in a rat model of orthopaedic implant-associated infection

Journal Article · · Journal of Antimicrobial Chemotherapy
ORCiD logo [1];  [2];  [2];  [3];  [4];  [5];  [3];  [3];  [6];  [7];  [8]
  1. Microbiology and Antibiotic Resistance Team (MicroART); Department of Veterinary Sciences; University of Trás-os-Montes and Alto Douro (UTAD); Vila Real; Portugal; Department of Genetics and Biotechnology; University of Trás-os-Montes and Alto Douro (UTAD); Vila Real; Portugal; Functional Genomics and Proteomics Unit; University of Trás-os-Montes and Alto Douro (UTAD); Vila Real; Portugal; Associated Laboratory for Green Chemistry (LAQV-REQUIMTE); University NOVA of Lisboa; Lisboa; Caparica; Portugal
  2. Angelini, Medical Department, C. Quebrada-Dafundo, Portugal
  3. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal, CECAV, Vila Real, Portugal
  4. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal, Department of Zootechnics, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
  5. Department of Zootechnics, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
  6. BIOSCOPE Group, LAQV@REQUIMTE, Chemistry Department, Faculty of Science and Technology, NOVA University of Lisbon, Almada, Portugal, Proteomass Scientific Society, Costa de Caparica, Portugal
  7. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal, Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal, Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal
  8. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal, Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal
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Abstract Objectives The aim of this study was to evaluate the efficacy of dalbavancin against MRSA biofilm-related infection in orthopaedic implants in vivo. Methods One MRSA strain isolated from human osteomyelitis was used to promote biofilm formation on the surface of screws. The implants were inserted in the proximal tibia under general anaesthesia. Thirty-nine Wistar rats were divided into three groups [control group (no treatment), Group 1 (7 days of treatment) and Group 2 (14 days of treatment)]; both treatment groups were administered dalbavancin intraperitoneally and euthanized after treatment. cfu of bacteria present in both the tibia and the implant were quantified. The infection severity was assessed by histopathology and scored from 0 (no infection) to 4 (severe infection). Results The high number of cfu/g and cfu/mL present in the control group indicated a well-established infection. There was a significant reduction in cfu in rats treated with dalbavancin both in the tibia (2.8 × 105 cfu/g) and the implant (1.1 × 106 cfu/mL) in Group 1 (1.8 × 103 cfu/g and 2.4 × 105 cfu/mL, respectively) and in Group 2 (8.2 cfu/g and 8.2 × 103 cfu/mL, respectively). Most animals from the control group presented an infection scored as 3 (severe). At the end of the experiment, most rats from Groups 1 and 2 presented an infection scored as 2 (moderate) and 0 (no infection), respectively. Conclusions Although there was a marked decrease in cfu number, signs of biofilm-induced infection prevailed after 14 days of treatment. Further studies should be carried out to evaluate the potential of dalbavancin in the treatment of bone and orthopaedic implant-associated MRSA infections.

Sponsoring Organization:
USDOE Office of Nuclear Energy (NE), Nuclear Fuel Cycle and Supply Chain
OSTI ID:
1619442
Journal Information:
Journal of Antimicrobial Chemotherapy, Journal Name: Journal of Antimicrobial Chemotherapy Journal Issue: 8 Vol. 75; ISSN 0305-7453
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United Kingdom
Language:
English

References (29)

Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material: DISPERSAL OF MRSA BIOFILMS journal July 2009
Recommendations for design and conduct of preclinical in vivo studies of orthopedic device-related infection: IN VIVO STUDIES OF ODRI journal February 2019
Application of a Rat Osteomyelitis Model to Comparein Vivoandin Vitrothe Antibiotic Efficacy against Bacteria with High Capacity to Form Biofilms journal October 1998
Dalbavancin reduces biofilms of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) journal November 2016
A simple infection model using pre-colonized implants to reproduce rat chronicStaphylococcus aureus osteomyelitis and study antibiotic treatment journal September 2001
Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials journal September 2012
In vitro activity of dalbavancin against biofilms of staphylococci isolated from prosthetic joint infections journal August 2016
Dalbavancin is active in vitro against biofilms formed by dalbavancin-susceptible enterococci journal January 2018
Dalbavancin for the management of gram-positive osteomyelitis: Effectiveness and potential utility journal March 2019
Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention journal December 2018
Antibiotic resistance of bacterial biofilms journal April 2010
First report of linezolid-resistant cfr-positive methicillin-resistant Staphylococcus aureus in humans in Portugal journal June 2019
The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection journal November 2015
Molecular mechanisms of antimicrobial tolerance and resistance in bacterial and fungal biofilms journal June 2014
Dalbavancin for treatment of implant-related methicillin-resistant Staphylococcus aureus osteomyelitis in an experimental rat model journal June 2018
The role of dalbavancin in the multi-disciplinary management of wound infections in orthopaedic surgery journal November 2017
Efficacy of dalbavancin in the treatment of MRSA rat sternal osteomyelitis with mediastinitis journal October 2015
Engineering Approaches for the Detection and Control of Orthopaedic Biofilm Infections journal January 2005
Daptomycin, Fosfomycin, or Both for Treatment of Methicillin-Resistant Staphylococcus aureus Osteomyelitis in an Experimental Rat Model journal August 2011
Extended-Duration Dosing and Distribution of Dalbavancin into Bone and Articular Tissue journal January 2015
In Vitro and In Vivo Biofilm Characterization of Methicillin-Resistant Staphylococcus aureus from Patients Associated with Pharyngitis Infection journal January 2016
Pharmacokinetic-Pharmacodynamic Modeling of Dalbavancin, a Novel Glycopeptide Antibiotic journal September 2008
Biofilm in Implant Infections: Its Production and Regulation journal November 2005
Etiology of Implant Orthopedic Infections: A Survey on 1027 Clinical Isolates journal November 2005
Investigation of methicillin-resistant Staphylococcus aureus among clinical isolates from humans and animals by culture methods and multiplex PCR journal October 2018
The influence of skeletal implants on incidence of infection. Experiments in a canine model. journal January 1985
Intra-Hospital, Inter-Hospital and Intercontinental Spread of ST78 MRSA From Two Neonatal Intensive Care Unit Outbreaks Established Using Whole-Genome Sequencing journal July 2018
Antibiotic Discovery: Combatting Bacterial Resistance in Cells and in Biofilm Communities journal March 2015
New agents approved for treatment of acute staphylococcal skin infections journal January 2016

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