Fungal ecological strategies reflected in gene transcription - a case study of two litter decomposers

Barbi, Florian; Kohler, Annegret; Barry, Kerrie; Baskaran, Preetisri; Daum, Chris; Fauchery, Laure; Ihrmark, Katarina; Kuo, Alan; LaButti, Kurt; Lipzen, Anna; Morin, Emmanuelle; Grigoriev, Igor V.; Henrissat, Bernard; Lindahl, Björn D.; Martin, Francis

doi:10.1111/1462-2920.14873

Title: Fungal ecological strategies reflected in gene transcription - a case study of two litter decomposers

Journal Article · 24 November 2019 · Environmental Microbiology

DOI: https://doi.org/10.1111/1462-2920.14873 · OSTI ID:1619166

^[1]; Kohler, Annegret ^[2]; Barry, Kerrie ^[3]; Baskaran, Preetisri ^[1]; Daum, Chris ^[3]; Fauchery, Laure ^[2]; Ihrmark, Katarina ^[1]; Kuo, Alan ^[3]; LaButti, Kurt ^[3]; Lipzen, Anna ^[3]; Morin, Emmanuelle ^[2]; Grigoriev, Igor V. ^[4]; Henrissat, Bernard ^[5]; Lindahl, Björn D. ^[6]; Martin, Francis ^[7]

Swedish Univ. of Agricultural Sciences, Uppsala (Sweden)
Univ. de Lorraine, Champenoux (France)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
Aix‐Marseille Univ., Marseille (France); INRA, Marseille (France); King Abdulaziz Univ., Jeddah (Saudi Arabia)
Department of Soil and EnvironmentSwedish University of Agricultural Sciences Box 7014, SE‐750 07 Uppsala Sweden
Univ. de Lorraine, Champenoux (France); Beijing Forestry Univ. (China)

Microbial communities interplay with their environment through their functional traits that can be a response or an effect on the environment. Here, we explore how a functional trait-the decomposition of organic matter, can be addressed based on genetic markers and how the expression of these markers reflect ecological strategies of two fungal litter decomposer Gymnopus androsaceus and Chalara longipes. We sequenced the genomes of these two fungi, as well as their transcriptomes at different steps of Pinus sylvestris needles decomposition in microcosms. Our results highlighted that if the gene content of the two species could indicate similar potential decomposition abilities, the expression levels of specific gene families belonging to the glycoside hydrolase category reflected contrasting ecological strategies. Actually, C. longipes, the weaker decomposer in this experiment, turned out to have a high content of genes involved in cell wall polysaccharides decomposition but low expression levels, reflecting a versatile ecology compare to the more competitive G. androsaceus with high expression levels of keystone functional genes. Finally, we established that sequential expression of genes coding for different components of the decomposer machinery indicated adaptation to chemical changes in the substrate as decomposition progressed.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); Swedish Research Council (SRC)

Grant/Contract Number:: AC02-05CH11231; 2011-1747; ANR-11-LABX-0002_ARBRE

OSTI ID:: 1619166

Alternate ID(s):: OSTI ID: 1577420

Journal Information:: Environmental Microbiology, Vol. 22, Issue 3; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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