Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

Daly, Paul; Peng, Mao; Casado López, Sara; Lipzen, Anna; Ng, Vivian; Singan, Vasanth R.; Wang, Mei; Grigoriev, Igor V.; de Vries, Ronald P.; Mäkelä, Miia R.

doi:10.1016/j.jbiotec.2019.11.014

Title: Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

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Abstract

Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. Finally, this study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.

Authors:

^[1]; Peng, Mao ^[1]; Casado López, Sara ^[1]; Lipzen, Anna ^[2]; Ng, Vivian ^[2]; Singan, Vasanth R. ^[2]; Wang, Mei ^[2]; Grigoriev, Igor V. ^[2];

^[3]; Mäkelä, Miia R. ^[4]

Utrecht Univ. (Netherlands)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Utrecht Univ. (Netherlands); Univ. of Helsinki (Finland)
Univ. of Helsinki (Finland)

Publication Date:: 2019-11-25

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC); Netherlands Scientific Organization; Academy of Finland

OSTI Identifier:: 1619153

Alternate Identifier(s):: OSTI ID: 1630445

Grant/Contract Number:: AC02-05CH11231; 308284; NWO 824.15.023

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Biotechnology

Additional Journal Information:: Journal Volume: 308; Journal Issue: C; Journal ID: ISSN 0168-1656

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; basidiomycete; aromatics; lignin; gene expression; wite-rot

Citation Formats


                    Daly, Paul, Peng, Mao, Casado López, Sara, Lipzen, Anna, Ng, Vivian, Singan, Vasanth R., Wang, Mei, Grigoriev, Igor V., de Vries, Ronald P., and Mäkelä, Miia R. Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens.  United States: N. p., 2019. 
Web.  https://doi.org/10.1016/j.jbiotec.2019.11.014.

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                    Daly, Paul, Peng, Mao, Casado López, Sara, Lipzen, Anna, Ng, Vivian, Singan, Vasanth R., Wang, Mei, Grigoriev, Igor V., de Vries, Ronald P., & Mäkelä, Miia R. Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens.  United States.  https://doi.org/10.1016/j.jbiotec.2019.11.014

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                    Daly, Paul, Peng, Mao, Casado López, Sara, Lipzen, Anna, Ng, Vivian, Singan, Vasanth R., Wang, Mei, Grigoriev, Igor V., de Vries, Ronald P., and Mäkelä, Miia R. Mon .  
"Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens".  United States.  https://doi.org/10.1016/j.jbiotec.2019.11.014.  https://www.osti.gov/servlets/purl/1619153.

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@article{osti_1619153,

  title        = {Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens},

  author       = {Daly, Paul and Peng, Mao and Casado López, Sara and Lipzen, Anna and Ng, Vivian and Singan, Vasanth R. and Wang, Mei and Grigoriev, Igor V. and de Vries, Ronald P. and Mäkelä, Miia R.},

  abstractNote = {Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. Finally, this study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.},

  doi          = {10.1016/j.jbiotec.2019.11.014},

  journal      = {Journal of Biotechnology},

  number       = C,

  volume       = 308,

  place        = {United States},

  year         = {2019},

  month        = {11}

}

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