Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM
Abstract
Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light. Moreover, many subtle plant phenotypes become intensified and thus can be studied in more detail. This observation has caused a paradigm shift within the photosynthesis research community and an increasing number of scientists are interested in using fluctuating light growth conditions. However, high installation costs for commercial controllable LED setups as well as costly phenotyping equipment can make it hard for small academic groups to compete in this emerging field.
- Authors:
- Publication Date:
- Research Org.:
- Washington State Univ., Pullman, WA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1618903
- Alternate Identifier(s):
- OSTI ID: 1735370
- Grant/Contract Number:
- SC0017160
- Resource Type:
- Published Article
- Journal Name:
- Plant Methods
- Additional Journal Information:
- Journal Name: Plant Methods Journal Volume: 15 Journal Issue: 1; Journal ID: ISSN 1746-4811
- Publisher:
- BioMed Central
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Photosynthesis; Phenotyping; Fluctuating light; Data analysis automation; Do-it-yourself; Maker movement
Citation Formats
Schneider, Dominik, Lopez, Laura S., Li, Meng, Crawford, Joseph D., Kirchhoff, Helmut, and Kunz, Hans-Henning. Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM. United Kingdom: N. p., 2019.
Web. doi:10.1186/s13007-019-0546-1.
Schneider, Dominik, Lopez, Laura S., Li, Meng, Crawford, Joseph D., Kirchhoff, Helmut, & Kunz, Hans-Henning. Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM. United Kingdom. https://doi.org/10.1186/s13007-019-0546-1
Schneider, Dominik, Lopez, Laura S., Li, Meng, Crawford, Joseph D., Kirchhoff, Helmut, and Kunz, Hans-Henning. Mon .
"Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM". United Kingdom. https://doi.org/10.1186/s13007-019-0546-1.
@article{osti_1618903,
title = {Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM},
author = {Schneider, Dominik and Lopez, Laura S. and Li, Meng and Crawford, Joseph D. and Kirchhoff, Helmut and Kunz, Hans-Henning},
abstractNote = {Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light. Moreover, many subtle plant phenotypes become intensified and thus can be studied in more detail. This observation has caused a paradigm shift within the photosynthesis research community and an increasing number of scientists are interested in using fluctuating light growth conditions. However, high installation costs for commercial controllable LED setups as well as costly phenotyping equipment can make it hard for small academic groups to compete in this emerging field.},
doi = {10.1186/s13007-019-0546-1},
journal = {Plant Methods},
number = 1,
volume = 15,
place = {United Kingdom},
year = {2019},
month = {12}
}
https://doi.org/10.1186/s13007-019-0546-1
Web of Science
Works referenced in this record:
Photosystem II core phosphorylation and photosynthetic acclimation require two different protein kinases
journal, October 2005
- Bonardi, Vera; Pesaresi, Paolo; Becker, Thomas
- Nature, Vol. 437, Issue 7062
A bestrophin-like protein modulates the proton motive force across the thylakoid membrane in Arabidopsis : Involvement of AtBest in fine-tuning of photosynthesis
journal, April 2016
- Duan, Zhikun; Kong, Fanna; Zhang, Lin
- Journal of Integrative Plant Biology, Vol. 58, Issue 10
Thioredoxins Play a Crucial Role in Dynamic Acclimation of Photosynthesis in Fluctuating Light
journal, January 2017
- Thormählen, Ina; Zupok, Arkadiusz; Rescher, Josephin
- Molecular Plant, Vol. 10, Issue 1
The Fluctuating Cell-Specific Light Environment and Its Effects on Cyanobacterial Physiology
journal, August 2019
- Andersson, Björn; Shen, Chen; Cantrell, Michael
- Plant Physiology, Vol. 181, Issue 2
PGR5 Is Involved in Cyclic Electron Flow around Photosystem I and Is Essential for Photoprotection in Arabidopsis
journal, August 2002
- Munekage, Yuri; Hojo, Masaya; Meurer, Jörg
- Cell, Vol. 110, Issue 3
The role of the xanthophyll cycle and of lutein in photoprotection of photosystem II
journal, January 2012
- Jahns, Peter; Holzwarth, Alfred R.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 1
scikit-image: image processing in Python
journal, January 2014
- van der Walt, Stéfan; Schönberger, Johannes L.; Nunez-Iglesias, Juan
- PeerJ, Vol. 2
PROTON GRADIENT REGULATION5 Is Essential for Proper Acclimation of Arabidopsis Photosystem I to Naturally and Artificially Fluctuating Light Conditions
journal, July 2012
- Suorsa, Marjaana; Järvi, Sari; Grieco, Michele
- The Plant Cell, Vol. 24, Issue 7
Dynamic Environmental Photosynthetic Imaging Reveals Emergent Phenotypes
journal, June 2016
- Cruz, Jeffrey A.; Savage, Linda J.; Zegarac, Robert
- Cell Systems, Vol. 2, Issue 6
PlantCV v2: Image analysis software for high-throughput plant phenotyping
journal, January 2017
- Gehan, Malia A.; Fahlgren, Noah; Abbasi, Arash
- PeerJ, Vol. 5
Fluctuating Light Interacts with Time of Day and Leaf Development Stage to Reprogram Gene Expression
journal, February 2019
- Schneider, Trang; Bolger, Anthony; Zeier, Jürgen
- Plant Physiology, Vol. 179, Issue 4
The regulation of the chloroplast proton motive force plays a key role for photosynthesis in fluctuating light
journal, June 2017
- Armbruster, Ute; Correa Galvis, Viviana; Kunz, Hans-Henning
- Current Opinion in Plant Biology, Vol. 37
Arabidopsis Mutants Define a Central Role for the Xanthophyll Cycle in the Regulation of Photosynthetic Energy Conversion
journal, July 1998
- Niyogi, Krishna K.; Grossman, Arthur R.; Björkman, Olle
- The Plant Cell, Vol. 10, Issue 7
Importance of Fluctuations in Light on Plant Photosynthetic Acclimation
journal, February 2017
- Vialet-Chabrand, Silvere; Matthews, Jack S. A.; Simkin, Andrew J.
- Plant Physiology, Vol. 173, Issue 4
Passive sampling of herbicides combined with effect analysis in algae using a novel high-throughput phytotoxicity assay (Maxi-Imaging-PAM)
journal, January 2006
- Escher, Beate I.; Quayle, Pam; Muller, Renee
- Journal of Environmental Monitoring, Vol. 8, Issue 4
Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications
journal, August 2013
- Murchie, E. H.; Lawson, T.
- Journal of Experimental Botany, Vol. 64, Issue 13
Thylakoid Protein Phosphorylation in Higher Plant Chloroplasts Optimizes Electron Transfer under Fluctuating Light
journal, December 2009
- Tikkanen, Mikko; Grieco, Michele; Kangasjärvi, Saijaliisa
- Plant Physiology, Vol. 152, Issue 2
Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts
journal, November 2018
- Trinh, Mai Duy Luu; Sato, Ryoichi; Masuda, Shinji
- Photosynthesis Research, Vol. 139, Issue 1-3
Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis
journal, May 2014
- Kunz, H. -H.; Gierth, M.; Herdean, A.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 20
Ion antiport accelerates photosynthetic acclimation in fluctuating light environments
journal, November 2014
- Armbruster, Ute; Carrillo, L. Ruby; Venema, Kees
- Nature Communications, Vol. 5, Issue 1
The Impacts of Fluctuating Light on Crop Performance
journal, November 2017
- Slattery, Rebecca A.; Walker, Berkley J.; Weber, Andreas P. M.
- Plant Physiology, Vol. 176, Issue 2
A pigment-binding protein essential for regulation of photosynthetic light harvesting
journal, January 2000
- Li, Xiao-Ping; Björkman, Olle; Shih, Connie
- Nature, Vol. 403, Issue 6768
A voltage-dependent chloride channel fine-tunes photosynthesis in plants
journal, May 2016
- Herdean, Andrei; Teardo, Enrico; Nilsson, Anders K.
- Nature Communications, Vol. 7, Issue 1
Raspberry Pi-powered imaging for plant phenotyping
journal, March 2018
- Tovar, Jose C.; Hoyer, J. Steen; Lin, Andy
- Applications in Plant Sciences, Vol. 6, Issue 3
Chlorophyll fluorescence—a practical guide
journal, April 2000
- Maxwell, Kate; Johnson, Giles N.
- Journal of Experimental Botany, Vol. 51, Issue 345
Photosynthesis in Arabidopsis Is Unaffected by the Function of the Vacuolar K + Channel TPK3
journal, May 2019
- Höhner, Ricarda; Galvis, Viviana Correa; Strand, Deserah D.
- Plant Physiology, Vol. 180, Issue 3
State transitions and light adaptation require chloroplast thylakoid protein kinase STN7
journal, February 2005
- Bellafiore, Stéphane; Barneche, Frédy; Peltier, Gilles
- Nature, Vol. 433, Issue 7028
K+ and Cl− channels/transporters independently fine-tune photosynthesis in plants
journal, June 2019
- Dukic, Emilija; Herdean, Andrei; Cheregi, Otilia
- Scientific Reports, Vol. 9, Issue 1
Steady-State Phosphorylation of Light-Harvesting Complex II Proteins Preserves Photosystem I under Fluctuating White Light
journal, October 2012
- Grieco, Michele; Tikkanen, Mikko; Paakkarinen, Virpi
- Plant Physiology, Vol. 160, Issue 4
Regulation and Levels of the Thylakoid K + /H + Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light
journal, May 2016
- Armbruster, Ute; Leonelli, Lauriebeth; Correa Galvis, Viviana
- Plant and Cell Physiology
Works referencing / citing this record:
The topology of plastid inner envelope potassium cation efflux antiporter KEA1 provides new insights into its regulatory features
journal, December 2019
- Bölter, Bettina; Mitterreiter, Melanie J.; Schwenkert, Serena
- Photosynthesis Research, Vol. 145, Issue 1
Comparative analysis of thylakoid protein complexes in state transition mutants nsi and stn7: focus on PSI and LHCII
journal, January 2020
- Koskela, Minna M.; Brünje, Annika; Ivanauskaite, Aiste
- Photosynthesis Research, Vol. 145, Issue 1