Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM

Schneider, Dominik; Lopez, Laura S.; Li, Meng; Crawford, Joseph D.; Kirchhoff, Helmut; Kunz, Hans-Henning

doi:10.1186/s13007-019-0546-1

Title: Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM

Journal Article · 23 December 2019 · Plant Methods

DOI: https://doi.org/10.1186/s13007-019-0546-1 · OSTI ID:1618903

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Washington State Univ., Pullman, WA (United States). Compact Plants Phenomics Center, Inst. of Biological Chemistry
Washington State Univ., Pullman, WA (United States)
Washington State Univ., Pullman, WA (United States). Inst. of Biological Chemistry

Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under fluctuating light despite being indistinguishable from wild-type plants under standard constant light. Moreover, many subtle plant phenotypes become intensified and thus can be studied in more detail. This observation has caused a paradigm shift within the photosynthesis research community and an increasing number of scientists are interested in using fluctuating light growth conditions. However, high installation costs for commercial controllable LED setups as well as costly phenotyping equipment can make it hard for small academic groups to compete in this emerging field.

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Research Organization:: Washington State Univ., Pullman, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0017160

OSTI ID:: 1618903

Alternate ID(s):: OSTI ID: 1735370

Journal Information:: Plant Methods, Vol. 15, Issue 1; ISSN 1746-4811

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 9 works

Citation information provided by
Web of Science

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Dynamic Environmental Photosynthetic Imaging Reveals Emergent Phenotypes Cruz, Jeffrey A.; Savage, Linda J.; Zegarac, Robert Cell Systems, Vol. 2, Issue 6 https://doi.org/10.1016/j.cels.2016.06.001	journal	June 2016
Thioredoxins Play a Crucial Role in Dynamic Acclimation of Photosynthesis in Fluctuating Light Thormählen, Ina; Zupok, Arkadiusz; Rescher, Josephin Molecular Plant, Vol. 10, Issue 1 https://doi.org/10.1016/j.molp.2016.11.012	journal	January 2017
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K+ and Cl− channels/transporters independently fine-tune photosynthesis in plants Dukic, Emilija; Herdean, Andrei; Cheregi, Otilia Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-44972-z	journal	June 2019
Passive sampling of herbicides combined with effect analysis in algae using a novel high-throughput phytotoxicity assay (Maxi-Imaging-PAM) Escher, Beate I.; Quayle, Pam; Muller, Renee Journal of Environmental Monitoring, Vol. 8, Issue 4 https://doi.org/10.1039/b517512g	journal	January 2006
Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis Kunz, H. -H.; Gierth, M.; Herdean, A. Proceedings of the National Academy of Sciences, Vol. 111, Issue 20 https://doi.org/10.1073/pnas.1323899111	journal	May 2014
Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications Murchie, E. H.; Lawson, T. Journal of Experimental Botany, Vol. 64, Issue 13 https://doi.org/10.1093/jxb/ert208	journal	August 2013
Thylakoid Protein Phosphorylation in Higher Plant Chloroplasts Optimizes Electron Transfer under Fluctuating Light Tikkanen, Mikko; Grieco, Michele; Kangasjärvi, Saijaliisa Plant Physiology, Vol. 152, Issue 2 https://doi.org/10.1104/pp.109.150250	journal	December 2009
Steady-State Phosphorylation of Light-Harvesting Complex II Proteins Preserves Photosystem I under Fluctuating White Light Grieco, Michele; Tikkanen, Mikko; Paakkarinen, Virpi Plant Physiology, Vol. 160, Issue 4 https://doi.org/10.1104/pp.112.206466	journal	October 2012
Importance of Fluctuations in Light on Plant Photosynthetic Acclimation Vialet-Chabrand, Silvere; Matthews, Jack S. A.; Simkin, Andrew J. Plant Physiology, Vol. 173, Issue 4 https://doi.org/10.1104/pp.16.01767	journal	February 2017
Arabidopsis Mutants Define a Central Role for the Xanthophyll Cycle in the Regulation of Photosynthetic Energy Conversion Niyogi, Krishna K.; Grossman, Arthur R.; Björkman, Olle The Plant Cell, Vol. 10, Issue 7 https://doi.org/10.1105/tpc.10.7.1121	journal	July 1998
A bestrophin-like protein modulates the proton motive force across the thylakoid membrane in Arabidopsis : Involvement of AtBest in fine-tuning of photosynthesis Duan, Zhikun; Kong, Fanna; Zhang, Lin Journal of Integrative Plant Biology, Vol. 58, Issue 10 https://doi.org/10.1111/jipb.12475	journal	April 2016

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The topology of plastid inner envelope potassium cation efflux antiporter KEA1 provides new insights into its regulatory features Bölter, Bettina; Mitterreiter, Melanie J.; Schwenkert, Serena Photosynthesis Research, Vol. 145, Issue 1 https://doi.org/10.1007/s11120-019-00700-2	journal	December 2019
Comparative analysis of thylakoid protein complexes in state transition mutants nsi and stn7: focus on PSI and LHCII Koskela, Minna M.; Brünje, Annika; Ivanauskaite, Aiste Photosynthesis Research, Vol. 145, Issue 1 https://doi.org/10.1007/s11120-020-00711-4	journal	January 2020

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Photosynthesis
Phenotyping
Fluctuating light
Data analysis automation
Do-it-yourself
Maker movement

MOESM1 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446902 The current record is supplemented by this text	text	January 2019
MOESM2 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446908 The current record is supplemented by this dataset	dataset	January 2019
MOESM2 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446908.v1 The current record is supplemented by this dataset	dataset	January 2019
MOESM3 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446914.v1 The current record is supplemented by this text	text	January 2019
MOESM4 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446917 The current record is supplemented by this dataset	dataset	January 2019
MOESM4 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446917.v1 The current record is supplemented by this dataset	dataset	January 2019
MOESM5 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446923 The current record is supplemented by this image	image	January 2019
MOESM5 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446923.v1 The current record is supplemented by this image	image	January 2019
MOESM6 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446932 The current record is supplemented by this image	image	January 2019
MOESM6 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446932.v1 The current record is supplemented by this image	image	January 2019
MOESM7 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446938 The current record is supplemented by this image	image	January 2019
MOESM7 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446938.v1 The current record is supplemented by this image	image	January 2019
Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.c.4795587 The current record is supplemented by this collection	collection	January 2019
Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.c.4795587.v1 The current record is supplemented by this collection	collection	January 2019
MOESM1 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446902.v1 The current record is supplemented by this text	text	January 2019
MOESM3 of Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM Schneider, Dominik; Lopez, Laura; Li, Meng https://doi.org/10.6084/m9.figshare.11446914 The current record is supplemented by this text	text	January 2019