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Title: Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Abstract

Background: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice. Results: Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues. Conclusion: Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass.

Authors:
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Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1618881
Alternate Identifier(s):
OSTI ID: 1477379
Grant/Contract Number:  
AC02-05CH11231; SC006904
Resource Type:
Published Article
Journal Name:
BMC Biotechnology (Online)
Additional Journal Information:
Journal Name: BMC Biotechnology (Online) Journal Volume: 18 Journal Issue: 1; Journal ID: ISSN 1472-6750
Publisher:
Springer Science + Business Media
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Li, Guotian, Jones, Kyle C., Eudes, Aymerick, Pidatala, Venkataramana R., Sun, Jian, Xu, Feng, Zhang, Chengcheng, Wei, Tong, Jain, Rashmi, Birdseye, Devon, Canlas, Patrick E., Baidoo, Edward E. K., Duong, Phat Q., Sharma, Manoj K., Singh, Seema, Ruan, Deling, Keasling, Jay D., Mortimer, Jenny C., Loqué, Dominique, Bartley, Laura E., Scheller, Henrik V., and Ronald, Pamela C. Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification. United Kingdom: N. p., 2018. Web. doi:10.1186/s12896-018-0464-8.
Li, Guotian, Jones, Kyle C., Eudes, Aymerick, Pidatala, Venkataramana R., Sun, Jian, Xu, Feng, Zhang, Chengcheng, Wei, Tong, Jain, Rashmi, Birdseye, Devon, Canlas, Patrick E., Baidoo, Edward E. K., Duong, Phat Q., Sharma, Manoj K., Singh, Seema, Ruan, Deling, Keasling, Jay D., Mortimer, Jenny C., Loqué, Dominique, Bartley, Laura E., Scheller, Henrik V., & Ronald, Pamela C. Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification. United Kingdom. doi:10.1186/s12896-018-0464-8.
Li, Guotian, Jones, Kyle C., Eudes, Aymerick, Pidatala, Venkataramana R., Sun, Jian, Xu, Feng, Zhang, Chengcheng, Wei, Tong, Jain, Rashmi, Birdseye, Devon, Canlas, Patrick E., Baidoo, Edward E. K., Duong, Phat Q., Sharma, Manoj K., Singh, Seema, Ruan, Deling, Keasling, Jay D., Mortimer, Jenny C., Loqué, Dominique, Bartley, Laura E., Scheller, Henrik V., and Ronald, Pamela C. Tue . "Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification". United Kingdom. doi:10.1186/s12896-018-0464-8.
@article{osti_1618881,
title = {Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification},
author = {Li, Guotian and Jones, Kyle C. and Eudes, Aymerick and Pidatala, Venkataramana R. and Sun, Jian and Xu, Feng and Zhang, Chengcheng and Wei, Tong and Jain, Rashmi and Birdseye, Devon and Canlas, Patrick E. and Baidoo, Edward E. K. and Duong, Phat Q. and Sharma, Manoj K. and Singh, Seema and Ruan, Deling and Keasling, Jay D. and Mortimer, Jenny C. and Loqué, Dominique and Bartley, Laura E. and Scheller, Henrik V. and Ronald, Pamela C.},
abstractNote = {Background: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice. Results: Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues. Conclusion: Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass.},
doi = {10.1186/s12896-018-0464-8},
journal = {BMC Biotechnology (Online)},
number = 1,
volume = 18,
place = {United Kingdom},
year = {2018},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s12896-018-0464-8

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Cited by: 3 works
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Works referenced in this record:

Expression of a bacterial 3-dehydroshikimate dehydratase reduces lignin content and improves biomass saccharification efficiency
journal, January 2015

  • Eudes, Aymerick; Sathitsuksanoh, Noppadon; Baidoo, Edward E. K.
  • Plant Biotechnology Journal, Vol. 13, Issue 9
  • DOI: 10.1111/pbi.12310

Ensembl Genomes 2016: more genomes, more complexity
journal, November 2015

  • Kersey, Paul Julian; Allen, James E.; Armean, Irina
  • Nucleic Acids Research, Vol. 44, Issue D1
  • DOI: 10.1093/nar/gkv1209

Two-year field analysis of reduced recalcitrance transgenic switchgrass
journal, April 2014

  • Baxter, Holly L.; Mazarei, Mitra; Labbe, Nicole
  • Plant Biotechnology Journal, Vol. 12, Issue 7
  • DOI: 10.1111/pbi.12195

Life-cycle energy and greenhouse gas emission impacts of different corn ethanol plant types
journal, April 2007


Genetic and biotechnological approaches for biofuel crop improvement
journal, April 2010


Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis
journal, February 2018

  • Biswal, Ajaya K.; Atmodjo, Melani A.; Li, Mi
  • Nature Biotechnology, Vol. 36, Issue 3
  • DOI: 10.1038/nbt.4067

Hemicelluloses
journal, June 2010


Switchgrass as an energy crop for biofuel production: A review of its ligno-cellulosic chemical properties
journal, January 2010

  • David, Kasi; Ragauskas, Arthur J.
  • Energy & Environmental Science, Vol. 3, Issue 9
  • DOI: 10.1039/b926617h

Linkage of p-coumaroyl and feruloyl groups to cell-wall polysaccharides of barley straw
journal, April 1986


Xylan biosynthesis
journal, April 2014


Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007

  • Chen, Fang; Dixon, Richard A.
  • Nature Biotechnology, Vol. 25, Issue 7, p. 759-761
  • DOI: 10.1038/nbt1316

Clustal W and Clustal X version 2.0
journal, September 2007


Modification of esterified cell wall phenolics increases vulnerability of tall fescue to herbivory by the fall armyworm
journal, March 2012


Activation of lignocellulosic biomass for higher sugar yields using aqueous ionic liquid at low severity process conditions
journal, August 2016

  • Parthasarathi, Ramakrishnan; Sun, Jian; Dutta, Tanmoy
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0561-7

Biomass recalcitrance: a multi-scale, multi-factor, and conversion-specific property: Fig. 1.
journal, June 2015

  • McCann, Maureen C.; Carpita, Nicholas C.
  • Journal of Experimental Botany, Vol. 66, Issue 14
  • DOI: 10.1093/jxb/erv267

Combining enhanced biomass density with reduced lignin level for improved forage quality
journal, July 2015

  • Gallego-Giraldo, Lina; Shadle, Gail; Shen, Hui
  • Plant Biotechnology Journal, Vol. 14, Issue 3
  • DOI: 10.1111/pbi.12439

An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14
journal, June 2015

  • Mortimer, Jenny C.; Faria-Blanc, Nuno; Yu, Xiaolan
  • The Plant Journal, Vol. 83, Issue 3
  • DOI: 10.1111/tpj.12898

Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
journal, January 2012

  • Withers, Saunia; Lu, Fachuang; Kim, Hoon
  • Journal of Biological Chemistry, Vol. 287, Issue 11, p. 8347-8355
  • DOI: 10.1074/jbc.M111.284497

Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production
journal, July 2011


Engineering of plant cell walls for enhanced biofuel production
journal, June 2015

  • Loqué, Dominique; Scheller, Henrik V.; Pauly, Markus
  • Current Opinion in Plant Biology, Vol. 25
  • DOI: 10.1016/j.pbi.2015.05.018

Genome-Wide Sequencing of 41 Rice (Oryza sativa L.) Mutated Lines Reveals Diverse Mutations Induced by Fast-Neutron Irradiation
journal, July 2016


Assessment of drought tolerance of 49 switchgrass (Panicum virgatum) genotypes using physiological and morphological parameters
journal, September 2015


Phytozome: a comparative platform for green plant genomics
journal, November 2011

  • Goodstein, David M.; Shu, Shengqiang; Howson, Russell
  • Nucleic Acids Research, Vol. 40, Issue D1
  • DOI: 10.1093/nar/gkr944

p -Coumaroyl-CoA:monolignol transferase (PMT) acts specifically in the lignin biosynthetic pathway in Brachypodium distachyon
journal, February 2014

  • Petrik, Deborah L.; Karlen, Steven D.; Cass, Cynthia L.
  • The Plant Journal, Vol. 77, Issue 5
  • DOI: 10.1111/tpj.12420

A Genomics Approach to Deciphering Lignin Biosynthesis in Switchgrass
journal, November 2013


Switchgrass SBP-box transcription factors PvSPL1 and 2 function redundantly to initiate side tillers and affect biomass yield of energy crop
journal, May 2016


Structural Redesigning Arabidopsis Lignins into Alkali-Soluble Lignins through the Expression of p- Coumaroyl-CoA:Monolignol Transferase PMT
journal, January 2016

  • Sibout, Richard; Le Bris, Philippe; Legée, Frédéric
  • Plant Physiology, Vol. 170, Issue 3
  • DOI: 10.1104/pp.15.01877

Plants to power: bioenergy to fuel the future
journal, August 2008


Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls
journal, January 2015

  • Vega-Sánchez, Miguel E.; Loqué, Dominique; Lao, Jeemeng
  • Plant Biotechnology Journal, Vol. 13, Issue 7
  • DOI: 10.1111/pbi.12326

Phenolics in Maize Genotypes Differing in Susceptibility to Gibberella Stalk Rot ( Fusarium graminearum Schwabe)
journal, June 2007

  • Santiago, Rogelio; Reid, Lana M.; Arnason, John T.
  • Journal of Agricultural and Food Chemistry, Vol. 55, Issue 13
  • DOI: 10.1021/jf070641e

Rhamnogalacturonan I in Solanum tuberosum tubers contains complex arabinogalactan structures
journal, May 2004


Lignification: Flexibility, Biosynthesis and Regulation
journal, August 2016


Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis
journal, November 2014

  • de Oliveira, Dyoni Matias; Finger-Teixeira, Aline; Rodrigues Mota, Thatiane
  • Plant Biotechnology Journal, Vol. 13, Issue 9
  • DOI: 10.1111/pbi.12292

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011

  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Feruloylation in Grasses: Current and Future Perspectives
journal, September 2009


Overexpression of the maize Corngrass1 microRNA prevents flowering, improves digestibility, and increases starch content of switchgrass
journal, October 2011

  • Chuck, G. S.; Tobias, C.; Sun, L.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 42, p. 17550-17555
  • DOI: 10.1073/pnas.1113971108

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
journal, March 1959


Monolignol ferulate conjugates are naturally incorporated into plant lignins
journal, October 2016

  • Karlen, Steven D.; Zhang, Chengcheng; Peck, Matthew L.
  • Science Advances, Vol. 2, Issue 10
  • DOI: 10.1126/sciadv.1600393

High throughput Agrobacterium-mediated switchgrass transformation
journal, March 2011


Decreased Polysaccharide Feruloylation Compromises Plant Cell Wall Integrity and Increases Susceptibility to Necrotrophic Fungal Pathogens
journal, May 2016

  • Reem, Nathan T.; Pogorelko, Gennady; Lionetti, Vincenzo
  • Frontiers in Plant Science, Vol. 7
  • DOI: 10.3389/fpls.2016.00630

Overexpression of a BAHD Acyltransferase, OsAt10 , Alters Rice Cell Wall Hydroxycinnamic Acid Content and Saccharification
journal, February 2013

  • Bartley, Laura E.; Peck, Matthew L.; Kim, Sung-Ryul
  • Plant Physiology, Vol. 161, Issue 4
  • DOI: 10.1104/pp.112.208694

Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production
journal, January 2013

  • Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-71

Standardization of Switchgrass Sample Collection for Cell Wall and Biomass Trait Analysis
journal, January 2013


Genetic manipulation of lignocellulosic biomass for bioenergy
journal, December 2015


Improvement of the Oryza sativa Nipponbare reference genome using next generation sequence and optical map data
journal, January 2013

  • Kawahara, Yoshihiro; de la Bastide, Melissa; Hamilton, John P.
  • Rice, Vol. 6, Issue 1
  • DOI: 10.1186/1939-8433-6-4

Field Evaluation of Transgenic Switchgrass Plants Overexpressing PvMYB4 for Reduced Biomass Recalcitrance
journal, January 2015

  • Baxter, Holly L.; Poovaiah, Charleson R.; Yee, Kelsey L.
  • BioEnergy Research, Vol. 8, Issue 3
  • DOI: 10.1007/s12155-014-9570-1

A Novel Bioinformatics Approach Identifies Candidate Genes for the Synthesis and Feruloylation of Arabinoxylan
journal, March 2007

  • Mitchell, R. A. C.; Dupree, P.; Shewry, P. R.
  • Plant Physiology, Vol. 144, Issue 1, p. 43-53
  • DOI: 10.1104/pp.106.094995

BAHD or SCPL acyltransferase? What a dilemma for acylation in the world of plant phenolic compounds
journal, June 2015

  • Bontpart, Thibaut; Cheynier, Véronique; Ageorges, Agnès
  • New Phytologist, Vol. 208, Issue 3
  • DOI: 10.1111/nph.13498

Polysaccharide Analysis Using Carbohydrate Gel Electrophoresis: A Method to Study Plant Cell Wall Polysaccharides and Polysaccharide Hydrolases
journal, January 2002

  • Goubet, Florence; Jackson, Peter; Deery, Michael J.
  • Analytical Biochemistry, Vol. 300, Issue 1
  • DOI: 10.1006/abio.2001.5444

Hydroxycinnamates in lignification
journal, August 2009


SMART: recent updates, new developments and status in 2015
journal, October 2014

  • Letunic, Ivica; Doerks, Tobias; Bork, Peer
  • Nucleic Acids Research, Vol. 43, Issue D1
  • DOI: 10.1093/nar/gku949

Unique aspects of the grass cell wall
journal, June 2008


XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan
journal, October 2012

  • Chiniquy, D.; Sharma, V.; Schultink, A.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 42
  • DOI: 10.1073/pnas.1202079109

    Works referencing / citing this record:

    Whole-Genome Sequencing Identifies a Rice Grain Shape Mutant, gs9–1
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