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Title: CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

Authors:
; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Washington Univ., St. Louis, MO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1618804
Alternate Identifier(s):
OSTI ID: 1375815
Resource Type:
Published Article
Journal Name:
Microbial Cell Factories
Additional Journal Information:
Journal Name: Microbial Cell Factories Journal Volume: 15 Journal Issue: 1; Journal ID: ISSN 1475-2859
Publisher:
Springer Science + Business Media
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Cyanobacteria; Synechococcus; CRISPR; Cas9; Genome modification

Citation Formats

Wendt, Kristen E., Ungerer, Justin, Cobb, Ryan E., Zhao, Huimin, and Pakrasi, Himadri B. CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973. United Kingdom: N. p., 2016. Web. doi:10.1186/s12934-016-0514-7.
Wendt, Kristen E., Ungerer, Justin, Cobb, Ryan E., Zhao, Huimin, & Pakrasi, Himadri B. CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973. United Kingdom. doi:10.1186/s12934-016-0514-7.
Wendt, Kristen E., Ungerer, Justin, Cobb, Ryan E., Zhao, Huimin, and Pakrasi, Himadri B. Thu . "CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973". United Kingdom. doi:10.1186/s12934-016-0514-7.
@article{osti_1618804,
title = {CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973},
author = {Wendt, Kristen E. and Ungerer, Justin and Cobb, Ryan E. and Zhao, Huimin and Pakrasi, Himadri B.},
abstractNote = {},
doi = {10.1186/s12934-016-0514-7},
journal = {Microbial Cell Factories},
number = 1,
volume = 15,
place = {United Kingdom},
year = {2016},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s12934-016-0514-7

Citation Metrics:
Cited by: 39 works
Citation information provided by
Web of Science

Figures / Tables:

Fig. 1 Fig. 1: Plasmids were generated using pCRISPomyces-2 backbone to engineer the ∆nblA line. a The nblA deletion plasmid including cas9 and b the nblA editing plasmid excluding cas9 are depicted

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    Works referencing / citing this record:

    Identifying the Metabolic Differences of a Fast-Growth Phenotype in Synechococcus UTEX 2973
    journal, January 2017

    • Mueller, Thomas J.; Ungerer, Justin L.; Pakrasi, Himadri B.
    • Scientific Reports, Vol. 7, Issue 1
    • DOI: 10.1038/srep41569

    Optimizing Systems for Cas9 Expression in Toxoplasma gondii
    journal, June 2019


    The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973
    journal, August 2018


    A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast
    journal, April 2018

    • Zhang, Xiao-Ran; He, Jia-Bei; Wang, Yi-Zheng
    • G3: Genes|Genomes|Genetics, Vol. 8, Issue 6
    • DOI: 10.1534/g3.118.200164

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