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Title: Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery

Abstract

Low-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid-AlCl3 catalyzed conversion to hydroxymethylfurfural (HMF) and levulinic acid (LA). However, lignin inhibits low-temperature swelling of TFA-treated intact wood particles from hybrid poplar (Populus tremula x P. alba) and results in greatly reduced yields of glucose or catalytic conversion compared to lignin-free cellulose. Previous studies have established that wood particles from transgenic lines of hybrid poplar with high syringyl (S) lignin content give greater glucose yields following enzymatic digestion. Low-temperature (-20 degrees C) treatment of S-lignin-rich poplar wood particles in TFA slightly increased yields of glucose from enzymatic digestions and HMF and LA from maleic acid-AlCl3 catalysis. Subsequent gelatinization at 55 °C resulted in over 80% digestion of cellulose in only 3 to 6 h with high-S-lignin wood, compared to 20-60% digestion in the wild-type poplar hybrid and transgenic lines high in guaiacyl lignin or 5-hydroxy-G lignin. Disassembly of lignin in woody particles by Ni/C catalytic systems improved yields of glucose by enzymatic digestion or catalytic conversion to HMF and LA. Although lignin was completely removed by Ni/C-catalyzed delignification (CDL) treatment, recalcitrance to enzymatic digestion of cellulose from themore » high-S lines was reduced compared to other lignin variants. However, cellulose still exhibited considerable recalcitrance to complete enzymatic digestion or catalytic conversion after complete delignification. Low-temperature swelling of the CDL-treated wood particles in TFA resulted in nearly complete enzymatic hydrolysis, regardless of original lignin composition. Genetic modification of lignin composition can enhance the portfolio of aromatic products obtained from lignocellulosic biomass while promoting disassembly into biofuel and bioproduct substrates. CDL enhances rates of enzymatic digestion and chemical conversion, but cellulose remains intrinsically recalcitrant. Cold TFA is sufficient to overcome this recalcitrance after CDL treatment. Our results inform a 'no carbon left behind' strategy to convert total woody biomass into lignin, cellulose, and hemicellulose value streams for the future biorefinery.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1618753
Alternate Identifier(s):
OSTI ID: 1544526
Report Number(s):
NREL/JA-2700-74364
Journal ID: ISSN 1754-6834; 171; PII: 1503
Grant/Contract Number:  
AC36-08GO28308; BEX-10734/13-9
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; cellulose; lignin; recalcitrance; catalysis; delignification; poplar

Citation Formats

Yang, Haibing, Zhang, Ximing, Luo, Hao, Liu, Baoyuan, Shiga, Tânia M., Li, Xu, Kim, Jeong Im, Rubinelli, Peter, Overton, Jonathan C., Subramanyam, Varun, Cooper, Bruce R., Mo, Huaping, Abu-Omar, Mahdi M., Chapple, Clint, Donohoe, Bryon S., Makowski, Lee, Mosier, Nathan S., McCann, Maureen C., Carpita, Nicholas C., and Meilan, Richard. Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery. Netherlands: N. p., 2019. Web. https://doi.org/10.1186/s13068-019-1503-y.
Yang, Haibing, Zhang, Ximing, Luo, Hao, Liu, Baoyuan, Shiga, Tânia M., Li, Xu, Kim, Jeong Im, Rubinelli, Peter, Overton, Jonathan C., Subramanyam, Varun, Cooper, Bruce R., Mo, Huaping, Abu-Omar, Mahdi M., Chapple, Clint, Donohoe, Bryon S., Makowski, Lee, Mosier, Nathan S., McCann, Maureen C., Carpita, Nicholas C., & Meilan, Richard. Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery. Netherlands. https://doi.org/10.1186/s13068-019-1503-y
Yang, Haibing, Zhang, Ximing, Luo, Hao, Liu, Baoyuan, Shiga, Tânia M., Li, Xu, Kim, Jeong Im, Rubinelli, Peter, Overton, Jonathan C., Subramanyam, Varun, Cooper, Bruce R., Mo, Huaping, Abu-Omar, Mahdi M., Chapple, Clint, Donohoe, Bryon S., Makowski, Lee, Mosier, Nathan S., McCann, Maureen C., Carpita, Nicholas C., and Meilan, Richard. Sat . "Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery". Netherlands. https://doi.org/10.1186/s13068-019-1503-y.
@article{osti_1618753,
title = {Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery},
author = {Yang, Haibing and Zhang, Ximing and Luo, Hao and Liu, Baoyuan and Shiga, Tânia M. and Li, Xu and Kim, Jeong Im and Rubinelli, Peter and Overton, Jonathan C. and Subramanyam, Varun and Cooper, Bruce R. and Mo, Huaping and Abu-Omar, Mahdi M. and Chapple, Clint and Donohoe, Bryon S. and Makowski, Lee and Mosier, Nathan S. and McCann, Maureen C. and Carpita, Nicholas C. and Meilan, Richard},
abstractNote = {Low-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid-AlCl3 catalyzed conversion to hydroxymethylfurfural (HMF) and levulinic acid (LA). However, lignin inhibits low-temperature swelling of TFA-treated intact wood particles from hybrid poplar (Populus tremula x P. alba) and results in greatly reduced yields of glucose or catalytic conversion compared to lignin-free cellulose. Previous studies have established that wood particles from transgenic lines of hybrid poplar with high syringyl (S) lignin content give greater glucose yields following enzymatic digestion. Low-temperature (-20 degrees C) treatment of S-lignin-rich poplar wood particles in TFA slightly increased yields of glucose from enzymatic digestions and HMF and LA from maleic acid-AlCl3 catalysis. Subsequent gelatinization at 55 °C resulted in over 80% digestion of cellulose in only 3 to 6 h with high-S-lignin wood, compared to 20-60% digestion in the wild-type poplar hybrid and transgenic lines high in guaiacyl lignin or 5-hydroxy-G lignin. Disassembly of lignin in woody particles by Ni/C catalytic systems improved yields of glucose by enzymatic digestion or catalytic conversion to HMF and LA. Although lignin was completely removed by Ni/C-catalyzed delignification (CDL) treatment, recalcitrance to enzymatic digestion of cellulose from the high-S lines was reduced compared to other lignin variants. However, cellulose still exhibited considerable recalcitrance to complete enzymatic digestion or catalytic conversion after complete delignification. Low-temperature swelling of the CDL-treated wood particles in TFA resulted in nearly complete enzymatic hydrolysis, regardless of original lignin composition. Genetic modification of lignin composition can enhance the portfolio of aromatic products obtained from lignocellulosic biomass while promoting disassembly into biofuel and bioproduct substrates. CDL enhances rates of enzymatic digestion and chemical conversion, but cellulose remains intrinsically recalcitrant. Cold TFA is sufficient to overcome this recalcitrance after CDL treatment. Our results inform a 'no carbon left behind' strategy to convert total woody biomass into lignin, cellulose, and hemicellulose value streams for the future biorefinery.},
doi = {10.1186/s13068-019-1503-y},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 12,
place = {Netherlands},
year = {2019},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
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https://doi.org/10.1186/s13068-019-1503-y

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Cited by: 6 works
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