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Title: Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization

Abstract

Engineering strategies to create promoters that are both higher strength and tunable in the presence of inexpensive compounds are of high importance to develop metabolic engineering technologies that can be commercialized. Lignocellulosic biomass stands out as the most abundant renewable feedstock for the production of biofuels and chemicals. However, lignin a major polymeric component of the biomass is made up of aromatic units and remains as an untapped resource. Novel synthetic biology tools for the expression of heterologous proteins are critical for the effective engineering of a microbe to valorize lignin. Furthermore, this study demonstrates the first successful attempt in the creation of engineered promoters that can be induced by aromatics present in lignocellulosic hydrolysates to increase heterologous protein production.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1618726
Alternate Identifier(s):
OSTI ID: 1473958
Report Number(s):
SAND-2018-9919J
Journal ID: ISSN 1754-6834; 182; PII: 1179
Grant/Contract Number:  
16-0758; AC02-05CH11231; AC04-94AL85000
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Lignin valorization; Promoter engineering; Biochemicals; Hybrid promoters; Phenolic inducers

Citation Formats

Varman, Arul M., Follenfant, Rhiannon, Liu, Fang, Davis, Ryan W., Lin, Yone K., and Singh, Seema. Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization. Netherlands: N. p., 2018. Web. doi:10.1186/s13068-018-1179-8.
Varman, Arul M., Follenfant, Rhiannon, Liu, Fang, Davis, Ryan W., Lin, Yone K., & Singh, Seema. Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization. Netherlands. doi:10.1186/s13068-018-1179-8.
Varman, Arul M., Follenfant, Rhiannon, Liu, Fang, Davis, Ryan W., Lin, Yone K., and Singh, Seema. Thu . "Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization". Netherlands. doi:10.1186/s13068-018-1179-8.
@article{osti_1618726,
title = {Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization},
author = {Varman, Arul M. and Follenfant, Rhiannon and Liu, Fang and Davis, Ryan W. and Lin, Yone K. and Singh, Seema},
abstractNote = {Engineering strategies to create promoters that are both higher strength and tunable in the presence of inexpensive compounds are of high importance to develop metabolic engineering technologies that can be commercialized. Lignocellulosic biomass stands out as the most abundant renewable feedstock for the production of biofuels and chemicals. However, lignin a major polymeric component of the biomass is made up of aromatic units and remains as an untapped resource. Novel synthetic biology tools for the expression of heterologous proteins are critical for the effective engineering of a microbe to valorize lignin. Furthermore, this study demonstrates the first successful attempt in the creation of engineered promoters that can be induced by aromatics present in lignocellulosic hydrolysates to increase heterologous protein production.},
doi = {10.1186/s13068-018-1179-8},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {Netherlands},
year = {2018},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s13068-018-1179-8

Citation Metrics:
Cited by: 4 works
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