Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan; Zienkiewicz, Krzysztof; Benning, Nils; Zienkiewicz, Agnieszka; Bonito, Gregory; Benning, Christoph

doi:10.1186/s13068-018-1172-2

Title: Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata

Abstract

Background: Although microalgal biofuels have potential advantages over conventional fossil fuels, high production costs limit their application in the market. We developed bio-flocculation and incubation methods for the marine alga, Nannochloropsis oceanica CCMP1779, and the oleaginous fungus, Mortierella elongata AG77, resulting in increased oil productivity. Results: By growing separately and then combining the cells, the M. elongata mycelium could efficiently capture N. oceanica due to an intricate cellular interaction between the two species leading to bio-flocculation. Use of a high-salt culture medium induced accumulation of triacylglycerol (TAG) and enhanced the contents of polyunsaturated fatty acids (PUFAs) including arachidonic acid and docosahexaenoic acid in M. elongata. To increase TAG productivity in the alga, we developed an effective, reduced nitrogen-supply regime based on ammonium in environmental photobioreactors. Under optimized conditions, N. oceanica produced high levels of TAG that could be indirectly monitored by following chlorophyll content. Combining N. oceanica and M. elongata to initiate bio-flocculation yielded high levels of TAG and total fatty acids, with ~ 15 and 22% of total dry weight (DW), respectively, as well as high levels of PUFAs. Genetic engineering of N. oceanica for higher TAG content in nutrient-replete medium was accomplished by overexpressing DGTT5, a gene encodingmore »« less

Authors:

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan; Zienkiewicz, Krzysztof; Benning, Nils; Zienkiewicz, Agnieszka; Bonito, Gregory;

Publication Date:: 2018-06-22

Research Org.:: Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; National Science Foundation (NSF)

OSTI Identifier:: 1618724

Alternate Identifier(s):: OSTI ID: 1459413

Grant/Contract Number:: FG02-91ER20021; FC02-07ER64494; SC0018409; 627266

Resource Type:: Published Article

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: Springer Science + Business Media

Country of Publication:: Netherlands

Language:: English

Subject:: 09 BIOMASS FUELS; Nannochloropsis; Mortierella; Bio‑flocculation; Polyunsaturated fatty acid; Triacylglycerol; Photobioreactor; Microalgae; Filamentous fungi; Cell–wall interaction; Biofuel; Nitrogen starvation

Citation Formats


                    Du, Zhi-Yan, Alvaro, Jonathan, Hyden, Brennan, Zienkiewicz, Krzysztof, Benning, Nils, Zienkiewicz, Agnieszka, Bonito, Gregory, and Benning, Christoph. Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata.  Netherlands: N. p., 2018. 
Web.  doi:10.1186/s13068-018-1172-2.

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                    Du, Zhi-Yan, Alvaro, Jonathan, Hyden, Brennan, Zienkiewicz, Krzysztof, Benning, Nils, Zienkiewicz, Agnieszka, Bonito, Gregory, & Benning, Christoph. Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata.  Netherlands.  https://doi.org/10.1186/s13068-018-1172-2

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                    Du, Zhi-Yan, Alvaro, Jonathan, Hyden, Brennan, Zienkiewicz, Krzysztof, Benning, Nils, Zienkiewicz, Agnieszka, Bonito, Gregory, and Benning, Christoph. Fri .  
"Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata".  Netherlands.  https://doi.org/10.1186/s13068-018-1172-2.

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@article{osti_1618724,

  title        = {Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata},

  author       = {Du, Zhi-Yan and Alvaro, Jonathan and Hyden, Brennan and Zienkiewicz, Krzysztof and Benning, Nils and Zienkiewicz, Agnieszka and Bonito, Gregory and Benning, Christoph},

  abstractNote = {Background: Although microalgal biofuels have potential advantages over conventional fossil fuels, high production costs limit their application in the market. We developed bio-flocculation and incubation methods for the marine alga, Nannochloropsis oceanica CCMP1779, and the oleaginous fungus, Mortierella elongata AG77, resulting in increased oil productivity. Results: By growing separately and then combining the cells, the M. elongata mycelium could efficiently capture N. oceanica due to an intricate cellular interaction between the two species leading to bio-flocculation. Use of a high-salt culture medium induced accumulation of triacylglycerol (TAG) and enhanced the contents of polyunsaturated fatty acids (PUFAs) including arachidonic acid and docosahexaenoic acid in M. elongata. To increase TAG productivity in the alga, we developed an effective, reduced nitrogen-supply regime based on ammonium in environmental photobioreactors. Under optimized conditions, N. oceanica produced high levels of TAG that could be indirectly monitored by following chlorophyll content. Combining N. oceanica and M. elongata to initiate bio-flocculation yielded high levels of TAG and total fatty acids, with ~ 15 and 22% of total dry weight (DW), respectively, as well as high levels of PUFAs. Genetic engineering of N. oceanica for higher TAG content in nutrient-replete medium was accomplished by overexpressing DGTT5, a gene encoding the type II acyl-CoA:diacylglycerol acyltransferase 5. Combined with bio-flocculation, this approach led to increased production of TAG under nutrient-replete conditions (~ 10% of DW) compared to the wild type (~ 6% of DW). In conclusion, the combined use of M. elongata and N. oceanica with available genomes and genetic engineering tools for both species opens up new avenues to improve biofuel productivity and allows for the engineering of polyunsaturated fatty acids.},

  doi          = {10.1186/s13068-018-1172-2},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 11,

  place        = {Netherlands},

  year         = {2018},

  month        = {6}

}

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Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (48.02 kB)
DOI: 10.6084/m9.figshare.6651188
This dataset is a supplement to the current record

MOESM1 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
figure, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (48.02 kB)
DOI: 10.6084/m9.figshare.6651188.v1
This figure is a supplement to the current record

MOESM2 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
figure, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (103.97 kB)
DOI: 10.6084/m9.figshare.6651215
This figure is a supplement to the current record

MOESM2 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
figure, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (103.97 kB)
DOI: 10.6084/m9.figshare.6651215.v1
This figure is a supplement to the current record

MOESM3 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
dataset, June 2018

Dan, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
Figshare, 148.05 kB
DOI: 10.6084/m9.figshare.6651233
This dataset is a supplement to the current record

MOESM3 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
dataset, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (148.05 kB)
DOI: 10.6084/m9.figshare.6651233.v1
This dataset is a supplement to the current record

MOESM4 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
figure, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (108.06 kB)
DOI: 10.6084/m9.figshare.6651251
This figure is a supplement to the current record

MOESM4 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
dataset, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
Figshare, 108.06 kB
DOI: 10.6084/m9.figshare.6651251.v1
This dataset is a supplement to the current record

MOESM5 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
dataset, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (98.82 kB)
DOI: 10.6084/m9.figshare.6651269
This dataset is a supplement to the current record

MOESM5 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
dataset, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
Figshare, 98.82 kB
DOI: 10.6084/m9.figshare.6651269.v1
This dataset is a supplement to the current record

MOESM6 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
figure, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (143.44 kB)
DOI: 10.6084/m9.figshare.6651281
This figure is a supplement to the current record

MOESM6 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
dataset, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
Figshare, 143.44 kB
DOI: 10.6084/m9.figshare.6651281.v1
This dataset is a supplement to the current record

MOESM7 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
figure, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (145.54 kB)
DOI: 10.6084/m9.figshare.6651296
This figure is a supplement to the current record

MOESM7 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
dataset, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (145.54 kB)
DOI: 10.6084/m9.figshare.6651296.v1
This dataset is a supplement to the current record

MOESM8 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
dataset, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
Figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 pdf (70.05 kB)
DOI: 10.6084/m9.figshare.6651314
This dataset is a supplement to the current record

MOESM8 of Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata
figure, June 2018

Du, Zhi-Yan; Alvaro, Jonathan; Hyden, Brennan
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1172-2, 1 PDF file (70.05 kB)
DOI: 10.6084/m9.figshare.6651314.v1
This figure is a supplement to the current record