Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass

Liu, Wusheng; Mazarei, Mitra; Ye, Rongjian; Peng, Yanhui; Shao, Yuanhua; Baxter, Holly L.; Sykes, Robert W.; Turner, Geoffrey B.; Davis, Mark F.; Wang, Zeng-Yu; Dixon, Richard A.; Stewart, C. Neal

doi:10.1186/s13068-018-1119-7

Title: Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass

Journal Article · 24 April 2018 · Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-018-1119-7 · OSTI ID:1618722

Liu, Wusheng ^[1]; Mazarei, Mitra ^[2]; Ye, Rongjian ^[3]; Peng, Yanhui ^[3]; Shao, Yuanhua ^[3]; Baxter, Holly L. ^[2]; Sykes, Robert W. ^[4]; Turner, Geoffrey B. ^[4]; Davis, Mark F. ^[4]; Wang, Zeng-Yu ^[5]; Dixon, Richard A. ^[6];

^[2]

Univ. of Tennessee, Knoxville, TN (United States). Dept. of Plant Sciences; North Carolina State Univ., Raleigh, NC (United States). Dept. of Horticultural Science
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Plant Sciences; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Plant Sciences
National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Noble Research Inst., Ardmore, OK (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of North Texas, Denton, TX (United States). BioDiscovery Inst. and Dept. of Biological Sciences

Genetic engineering of switchgrass (Panicum virgatum L.) for reduced cell wall recalcitrance and improved biofuel production has been a long pursued goal. Up to now, constitutive promoters have been used to direct the expression of cell wall biosynthesis genes toward attaining that goal. While generally sufficient to gauge a transgene's effects in the heterologous host, constitutive overexpression often leads to undesirable plant phenotypic effects. Green tissue-specific promoters from switchgrass are potentially valuable to directly alter cell wall traits exclusively in harvestable aboveground biomass while not changing root phenotypes. We identified and functionally characterized three switchgrass green tissue-specific promoters and assessed marker gene expression patterns and intensity in stably transformed rice (Oryza sativa L.), and then used them to direct the expression of the switchgrass MYB4 (PvMYB4) transcription factor gene in transgenic switchgrass to endow reduced recalcitrance in aboveground biomass. These promoters correspond to photosynthesis-related light-harvesting complex II chlorophyll-a/b binding gene (PvLhcb), phosphoenolpyruvate carboxylase (PvPEPC), and the photosystem II 10 kDa R subunit (PvPsbR). Real-time RT-PCR analysis detected their strong expression in the aboveground tissues including leaf blades, leaf sheaths, internodes, inflorescences, and nodes of switchgrass, which was tightly up-regulated by light. Stable transgenic rice expressing the GUS reporter under the control of each promoter (756-2005 bp in length) further confirmed their strong expression patterns in leaves and stems. With the exception of the serial promoter deletions of PvLhcb, all GUS marker patterns under the control of each 5'-end serial promoter deletion were not different from that conveyed by their respective promoters. All of the shortest promoter fragments (199-275 bp in length) conveyed strong green tissue-specific GUS expression in transgenic rice. PvMYB4 is a master repressor of lignin biosynthesis. The green tissue-specific expression of PvMYB4 via each promoter in transgenic switchgrass led to significant gains in saccharification efficiency, decreased lignin, and decreased S/G lignin ratios. In contrast to constitutive overexpression of PvMYB4, which negatively impacts switchgrass root growth, plant growth was not compromised in green tissue-expressed PvMYB4 switchgrass plants in the current study. Each of the newly described green tissue-specific promoters from switchgrass has utility to change cell wall biosynthesis exclusively in aboveground harvestable biomass without altering root systems. The truncated green tissue promoters are very short and should be useful for targeted expression in a number of monocots to improve shoot traits while restricting gene expression from roots. Green tissue-specific expression of PvMYB4 is an effective strategy for improvement of transgenic feedstocks.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Office of Biological and Environmental Research; USDOE

Grant/Contract Number:: AC36-08GO28308; PS02-06ER64304

OSTI ID:: 1618722

Alternate ID(s):: OSTI ID: 1437560

Report Number(s):: NREL/JA-2700-71538

Journal Information:: Biotechnology for Biofuels, Vol. 11, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 11 works

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Production of polyhydroxybutyrate in switchgrass, a value-added co-product in an important lignocellulosic biomass crop Somleva, Maria N.; Snell, Kristi D.; Beaulieu, Julie J. Plant Biotechnology Journal, Vol. 6, Issue 7 https://doi.org/10.1111/j.1467-7652.2008.00350.x	journal	September 2008
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Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass [Supplementary Data] Liu, Wusheng; Mazarei, Mitra; Ye, Rongjian Figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1119-7, 1 doc file (1.58 MB) https://doi.org/10.6084/m9.figshare.c.4078622.v1	dataset	April 2018

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09 BIOMASS FUELS
green tissue-specific promoter
transgenic rice
transgenic switchgrass

MOESM1 of Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass Liu, Wusheng; Mazarei, Mitra; Ye, Rongjian https://doi.org/10.6084/m9.figshare.6175205.v1 The current record is supplemented by this dataset	dataset	April 2018
Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass [Supplementary Data] Liu, Wusheng; Mazarei, Mitra; Ye, Rongjian https://doi.org/10.6084/M9.FIGSHARE.C.4078622 The current record is supplemented by this dataset	dataset	April 2018
Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass [Supplementary Data] Liu, Wusheng; Mazarei, Mitra; Ye, Rongjian https://doi.org/10.6084/m9.figshare.c.4078622.v1 The current record is supplemented by this dataset	dataset	April 2018
MOESM1 of Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass Liu, Wusheng; Mazarei, Mitra; Ye, Rongjian https://doi.org/10.6084/m9.figshare.6175205 The current record is supplemented by this dataset	dataset	April 2018

Title: Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass

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