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Title: In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions

Abstract

Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophyte Cellvibrio japonicus has emerged as a genetically tractable model system to study biomass saccharification, in part due to its innate capacity to utilize a wide range of plant polysaccharides for growth. Whereas the downstream debranching enzymes of the xyloglucan utilization system of C. japonicus have been functionally characterized, the requisite backbone-cleaving endo-xyloglucanases were unresolved.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Maryland, Baltimore, MD (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1618718
Alternate Identifier(s):
OSTI ID: 1504757
Grant/Contract Number:  
SC0014183
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Xyloglucan; Saccharification; Glycoside hydrolase; Cellvibrio japonicus; Saprophyte

Citation Formats

Attia, Mohamed A., Nelson, Cassandra E., Offen, Wendy A., Jain, Namrata, Davies, Gideon J., Gardner, Jeffrey G., and Brumer, Harry. In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions. Netherlands: N. p., 2018. Web. doi:10.1186/s13068-018-1039-6.
Attia, Mohamed A., Nelson, Cassandra E., Offen, Wendy A., Jain, Namrata, Davies, Gideon J., Gardner, Jeffrey G., & Brumer, Harry. In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions. Netherlands. doi:10.1186/s13068-018-1039-6.
Attia, Mohamed A., Nelson, Cassandra E., Offen, Wendy A., Jain, Namrata, Davies, Gideon J., Gardner, Jeffrey G., and Brumer, Harry. Sat . "In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions". Netherlands. doi:10.1186/s13068-018-1039-6.
@article{osti_1618718,
title = {In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions},
author = {Attia, Mohamed A. and Nelson, Cassandra E. and Offen, Wendy A. and Jain, Namrata and Davies, Gideon J. and Gardner, Jeffrey G. and Brumer, Harry},
abstractNote = {Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophyte Cellvibrio japonicus has emerged as a genetically tractable model system to study biomass saccharification, in part due to its innate capacity to utilize a wide range of plant polysaccharides for growth. Whereas the downstream debranching enzymes of the xyloglucan utilization system of C. japonicus have been functionally characterized, the requisite backbone-cleaving endo-xyloglucanases were unresolved.},
doi = {10.1186/s13068-018-1039-6},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {Netherlands},
year = {2018},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s13068-018-1039-6

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Cited by: 8 works
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