skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery

Abstract

Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from species Agave tequilana and Agave salmiana were subjected to pretreatment using the ammonia fiber expansion (AFEX) process. The pretreatment conditions were optimized using a response surface design methodology. We also identified commercial enzyme mixtures that maximize sugar yields for AFEX-pretreated agave bagasse and leaf matter, at ~ 6% glucan (w/w) loading enzymatic hydrolysis. Finally, the pretreated agave hydrolysates (at a total solids loading of ~ 20%) were used for ethanol fermentation using the glucose- and xylose-consuming strain Saccharomyces cerevisiae 424A (LNH-ST), to determine ethanol yields at industrially relevant conditions.

Authors:
ORCiD logo; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1618714
Alternate Identifier(s):
OSTI ID: 1504761
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; Agave; Biomass; Pretreatment; AFEX; Enzymatic hydrolysis; Cellulase; Fermentation; Biofuel; Lignocellulosic; Ethanol

Citation Formats

Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, and Balan, Venkatesh. Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery. Netherlands: N. p., 2018. Web. doi:10.1186/s13068-017-0995-6.
Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, & Balan, Venkatesh. Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery. Netherlands. doi:10.1186/s13068-017-0995-6.
Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, and Balan, Venkatesh. Tue . "Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery". Netherlands. doi:10.1186/s13068-017-0995-6.
@article{osti_1618714,
title = {Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery},
author = {Flores-Gómez, Carlos A. and Escamilla Silva, Eleazar M. and Zhong, Cheng and Dale, Bruce E. and da Costa Sousa, Leonardo and Balan, Venkatesh},
abstractNote = {Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from species Agave tequilana and Agave salmiana were subjected to pretreatment using the ammonia fiber expansion (AFEX) process. The pretreatment conditions were optimized using a response surface design methodology. We also identified commercial enzyme mixtures that maximize sugar yields for AFEX-pretreated agave bagasse and leaf matter, at ~ 6% glucan (w/w) loading enzymatic hydrolysis. Finally, the pretreated agave hydrolysates (at a total solids loading of ~ 20%) were used for ethanol fermentation using the glucose- and xylose-consuming strain Saccharomyces cerevisiae 424A (LNH-ST), to determine ethanol yields at industrially relevant conditions.},
doi = {10.1186/s13068-017-0995-6},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {Netherlands},
year = {2018},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s13068-017-0995-6

Citation Metrics:
Cited by: 9 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose
journal, January 2009

  • Kristensen, Jan B.; Felby, Claus; Jørgensen, Henning
  • Biotechnology for Biofuels, Vol. 2, Issue 1
  • DOI: 10.1186/1754-6834-2-11

Next-generation ammonia pretreatment enhances cellulosic biofuel production
journal, January 2016

  • da Costa Sousa, Leonardo; Jin, Mingjie; Chundawat, Shishir P. S.
  • Energy & Environmental Science, Vol. 9, Issue 4
  • DOI: 10.1039/C5EE03051J

Toward lower cost cellulosic biofuel production using ammonia based pretreatment technologies
journal, January 2016

  • Jin, Mingjie; da Costa Sousa, Leonardo; Schwartz, Christopher
  • Green Chemistry, Vol. 18, Issue 4
  • DOI: 10.1039/C5GC02433A

Ethanolic fermentation of hydrolysates from ammonia fiber expansion (AFEX) treated corn stover and distillers grain without detoxification and external nutrient supplementation
journal, January 2007

  • Lau, Ming W.; Dale, Bruce E.; Balan, Venkatesh
  • Biotechnology and Bioengineering, Vol. 99, Issue 3
  • DOI: 10.1002/bit.21609

Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects
journal, January 2016


‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies
journal, June 2009

  • da Costa Sousa, Leonardo; Chundawat, Shishir PS; Balan, Venkatesh
  • Current Opinion in Biotechnology, Vol. 20, Issue 3
  • DOI: 10.1016/j.copbio.2009.05.003

Lignocellulosic Biomass Pretreatment Using AFEX
book, January 2009


Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment
journal, January 2011

  • Chundawat, Shishir P. S.; Donohoe, Bryon S.; da Costa Sousa, Leonardo
  • Energy & Environmental Science, Vol. 4, Issue 3, 973
  • DOI: 10.1039/c0ee00574f

Toward systems‐level analysis of agricultural production from crassulacean acid metabolism ( CAM ): scaling from cell to commercial production
journal, June 2015

  • Davis, Sarah C.; Ming, Ray; LeBauer, David S.
  • New Phytologist, Vol. 208, Issue 1
  • DOI: 10.1111/nph.13522

Improving the performance of enzymes in hydrolysis of high solids paper pulp derived from MSW
journal, January 2013

  • Puri, Dhivya J.; Heaven, Sonia; Banks, Charles J.
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-107

Evaluation of ammonia fibre expansion (AFEX) pretreatment for enzymatic hydrolysis of switchgrass harvested in different seasons and locations
journal, January 2010

  • Bals, Bryan; Rogers, Chad; Jin, Mingjie
  • Biotechnology for Biofuels, Vol. 3, Issue 1
  • DOI: 10.1186/1754-6834-3-1

Effects of the pretreatment method on high solids enzymatic hydrolysis and ethanol fermentation of the cellulosic fraction of sugarcane bagasse
journal, September 2015

  • Martins, Luiza Helena da Silva; Rabelo, Sarita Cândida; Costa, Aline Carvalho da
  • Bioresource Technology, Vol. 191
  • DOI: 10.1016/j.biortech.2015.05.024

Utilization of byproducts from the tequila industry: part 1: agave bagasse as a raw material for animal feeding and fiberboard production
journal, March 2001


Potential of Plants from the Genus Agave as Bioenergy Crops
journal, October 2011


A packed bed Ammonia Fiber Expansion reactor system for pretreatment of agricultural residues at regional depots
journal, January 2013

  • Campbell, Timothy J.; Teymouri, Farzaneh; Bals, Bryan
  • Biofuels, Vol. 4, Issue 1
  • DOI: 10.4155/bfs.12.71

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Rheology of corn stover slurries at high solids concentrations – Effects of saccharification and particle size
journal, January 2009


Development of Agave as a dedicated biomass source: production of biofuels from whole plants
journal, May 2015

  • Mielenz, Jonathan R.; Rodriguez, Miguel; Thompson, Olivia A.
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0261-8

Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEX TM -pre-treated biomass
journal, April 2015

  • Pattathil, Sivakumar; Hahn, Michael G.; Dale, Bruce E.
  • Journal of Experimental Botany, Vol. 66, Issue 14
  • DOI: 10.1093/jxb/erv107

Optimizing harvest of corn stover fractions based on overall sugar yields following ammonia fiber expansion pretreatment and enzymatic hydrolysis
journal, January 2009

  • Garlock, Rebecca J.; Chundawat, Shishir PS; Balan, Venkatesh
  • Biotechnology for Biofuels, Vol. 2, Issue 1
  • DOI: 10.1186/1754-6834-2-29

Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification
journal, August 2000


Comparative material balances around pretreatment technologies for the conversion of switchgrass to soluble sugars
journal, December 2011


Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
journal, July 2011


Designer synthetic media for studying microbial-catalyzed biofuel production
journal, January 2015

  • Tang, Xiaoyu; da Costa Sousa, Leonardo; Jin, Mingjie
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-014-0179-6

Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010


Life cycle energy and greenhouse gas analysis for agave-derived bioethanol
journal, January 2011

  • Yan, Xiaoyu; Tan, Daniel K. Y.; Inderwildi, Oliver R.
  • Energy & Environmental Science, Vol. 4, Issue 9
  • DOI: 10.1039/c1ee01107c

The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials
journal, January 2016


Tequilana weber Agave Bagasse Enzymatic Hydrolysis for the Production of Fermentable Sugars: Oxidative-Alkaline Pretreatment and Kinetic Modeling
journal, May 2016

  • Velázquez-Valadez, Ulises; Farías-Sánchez, Juan Carlos; Vargas-Santillán, Alfonso
  • BioEnergy Research, Vol. 9, Issue 4
  • DOI: 10.1007/s12155-016-9757-8

Enzymatic hydrolysis at high-solids loadings for the conversion of agave bagasse to fuel ethanol
journal, January 2014


Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status
journal, October 2006

  • van Maris, Antonius J. A.; Abbott, Derek A.; Bellissimi, Eleonora
  • Antonie van Leeuwenhoek, Vol. 90, Issue 4
  • DOI: 10.1007/s10482-006-9085-7

Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments
journal, July 2016


Effects of alkaline or liquid-ammonia treatment on crystalline cellulose: changes in crystalline structure and effects on enzymatic digestibility
journal, January 2011

  • Mittal, Ashutosh; Katahira, Rui; Himmel, Michael E.
  • Biotechnology for Biofuels, Vol. 4, Issue 1
  • DOI: 10.1186/1754-6834-4-41

Restructuring the Crystalline Cellulose Hydrogen Bond Network Enhances Its Depolymerization Rate
journal, July 2011

  • Chundawat, Shishir P. S.; Bellesia, Giovanni; Uppugundla, Nirmal
  • Journal of the American Chemical Society, Vol. 133, Issue 29
  • DOI: 10.1021/ja2011115

De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana
journal, January 2013


A new lignocellulosic biomass deconstruction process combining thermo-mechano chemical action and bio-catalytic enzymatic hydrolysis in a twin-screw extruder
journal, April 2014


The global potential for Agave as a biofuel feedstock: AGAVE AS A BIOFUEL FEEDSTOCK
journal, November 2010


Enzymatic hydrolysis of biomass at high-solids loadings – A review
journal, September 2013


Comparison of colorimetric and HPLC techniques for quantitating the carbohydrate components of steam-treated wood: Scientific note
journal, April 1988

  • Irick, T. J.; West, K.; Brownell, H. H.
  • Applied Biochemistry and Biotechnology, Vol. 17, Issue 1-3
  • DOI: 10.1007/BF02779152

Efficient chemical and enzymatic saccharification of the lignocellulosic residue from Agave tequilana bagasse to produce ethanol by Pichia caribbica
journal, November 2010

  • Saucedo-Luna, Jaime; Castro-Montoya, Agustin Jaime; Martinez-Pacheco, Mauro Manuel
  • Journal of Industrial Microbiology & Biotechnology, Vol. 38, Issue 6
  • DOI: 10.1007/s10295-010-0853-z

Comparison of the impact of ionic liquid pretreatment on recalcitrance of agave bagasse and switchgrass
journal, January 2013


Utilization of by-products from the tequila industry. Part 2: potential value of Agave tequilana Weber azul leaves
journal, April 2001


Review of US and EU initiatives toward development, demonstration, and commercialization of lignocellulosic biofuels
journal, August 2013

  • Balan, Venkatesh; Chiaramonti, David; Kumar, Sandeep
  • Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 6
  • DOI: 10.1002/bbb.1436

Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production
journal, January 2010

  • Lau, Ming W.; Gunawan, Christa; Balan, Venkatesh
  • Biotechnology for Biofuels, Vol. 3, Issue 1
  • DOI: 10.1186/1754-6834-3-11

Process options for conversion of Agave tequilana leaves into bioethanol
journal, June 2016


Multifaceted characterization of cell wall decomposition products formed during ammonia fiber expansion (AFEX) and dilute acid based pretreatments
journal, November 2010


Model-Based Fed-Batch for High-Solids Enzymatic Cellulose Hydrolysis
journal, May 2008

  • Hodge, David B.; Karim, M. Nazmul; Schell, Daniel J.
  • Applied Biochemistry and Biotechnology, Vol. 152, Issue 1
  • DOI: 10.1007/s12010-008-8217-0

Hydrolysis of Agave fourcroydes Lemaire (henequen) leaf juice and fermentation with Kluyveromyces marxianus for ethanol production
journal, January 2014

  • Villegas-Silva, Pablo A.; Toledano-Thompson, Tanit; Canto-Canché, Blondy B.
  • BMC Biotechnology, Vol. 14, Issue 1
  • DOI: 10.1186/1472-6750-14-14

Agave as a biofuel feedstock in Australia: AGAVE IN AUSTRALIA
journal, December 2010


Techno-Economic Evaluation of Producing Ethanol from Softwood: Comparison of SSF and SHF and Identification of Bottlenecks
journal, January 2003

  • Wingren, Anders; Galbe, Mats; Zacchi, Guido
  • Biotechnology Progress, Vol. 19, Issue 4
  • DOI: 10.1021/bp0340180

A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass
journal, January 2016


Pretreatment: the key to unlocking low-cost cellulosic ethanol
journal, January 2008

  • Yang, Bin; Wyman, Charles E.
  • Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 1
  • DOI: 10.1002/bbb.49

Chemical composition and characterization of cellulose for Agave as a fast-growing, drought-tolerant biofuels feedstock
journal, January 2012

  • Li, Hongjia; Foston, Marcus B.; Kumar, Rajeev
  • RSC Advances, Vol. 2, Issue 11
  • DOI: 10.1039/c2ra20557b

    Works referencing / citing this record:

    Use of Euphorbia sp. (Euphorbiaceae) as biofuel feedstock for semi-arid and arid lands
    journal, December 2018


    The Role of Sustainability Analysis in the Revalorization of Tequila Residues and Wastes Using Biorefineries
    journal, July 2019