Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery

doi:10.1186/s13068-017-0995-6

Title: Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery

Abstract

Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from species Agave tequilana and Agave salmiana were subjected to pretreatment using the ammonia fiber expansion (AFEX) process. The pretreatment conditions were optimized using a response surface design methodology. We also identified commercial enzyme mixtures that maximize sugar yields for AFEX-pretreated agave bagasse and leaf matter, at ~ 6% glucan (w/w) loading enzymatic hydrolysis. Finally, the pretreated agave hydrolysates (at a total solids loading of ~ 20%) were used for ethanol fermentation using the glucose- and xylose-consuming strain Saccharomyces cerevisiae 424A (LNH-ST), to determine ethanol yields at industrially relevant conditions.

Authors:

; Escamilla Silva, Eleazar M.; Zhong, Cheng; Dale, Bruce E.; da Costa Sousa, Leonardo;

Publication Date:: 2018-01-16

Research Org.:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1618714

Alternate Identifier(s):: OSTI ID: 1504761

Grant/Contract Number:: FC02-07ER64494

Resource Type:: Published Article

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: Springer Science + Business Media

Country of Publication:: Netherlands

Language:: English

Subject:: 09 BIOMASS FUELS; Agave; Biomass; Pretreatment; AFEX; Enzymatic hydrolysis; Cellulase; Fermentation; Biofuel; Lignocellulosic; Ethanol

Citation Formats


                    Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, and Balan, Venkatesh. Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery.  Netherlands: N. p., 2018. 
        Web.  doi:10.1186/s13068-017-0995-6.

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                    Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, & Balan, Venkatesh. Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery.  Netherlands.  doi:10.1186/s13068-017-0995-6.

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                    Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, and Balan, Venkatesh. Tue .  
        "Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery".  Netherlands.  doi:10.1186/s13068-017-0995-6.

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@article{osti_1618714,

  title        = {Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery},

  author       = {Flores-Gómez, Carlos A. and Escamilla Silva, Eleazar M. and Zhong, Cheng and Dale, Bruce E. and da Costa Sousa, Leonardo and Balan, Venkatesh},

  abstractNote = {Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from species Agave tequilana and Agave salmiana were subjected to pretreatment using the ammonia fiber expansion (AFEX) process. The pretreatment conditions were optimized using a response surface design methodology. We also identified commercial enzyme mixtures that maximize sugar yields for AFEX-pretreated agave bagasse and leaf matter, at ~ 6% glucan (w/w) loading enzymatic hydrolysis. Finally, the pretreated agave hydrolysates (at a total solids loading of ~ 20%) were used for ethanol fermentation using the glucose- and xylose-consuming strain Saccharomyces cerevisiae 424A (LNH-ST), to determine ethanol yields at industrially relevant conditions.},

  doi          = {10.1186/s13068-017-0995-6},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 11,

  place        = {Netherlands},

  year         = {2018},

  month        = {1}

}

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DOI: 10.1186/s13068-017-0995-6

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