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Title: Undefined cellulase formulations hinder scientific reproducibility

Abstract

In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel production. For the effective comparison of one cellulase preparation to another, cellulase assays are typically carried out with one or more engineered cellulase formulations or natural exoproteomes of known performance serving as positive controls. When these formulations have unknown composition, as is the case with several widely used commercial products, it becomes impossible to compare or reproduce work done today to work done in the future, where, for example, such preparations may not be available. Therefore, being a critical tenet of science publishing, experimental reproducibility is endangered by the continued use of these undisclosed products. We propose the introduction of standard procedures and materials to produce specific and reproducible cellulase formulations. These formulations are to serve as yardsticks to measure improvements and performance of new cellulase formulations.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1618705
Alternate Identifier(s):
OSTI ID: 1414374
Report Number(s):
NREL/JA-2700-70611
Journal ID: ISSN 1754-6834; 283; s13068-017-0974-y; PII: 974
Grant/Contract Number:  
Contract DE-AC36–08GO28308; AC36-08GO28308
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; cellulase; cellulose; biofuels; assays; commercial cellulase formulations

Citation Formats

Himmel, Michael E., Abbas, Charles A., Baker, John O., Bayer, Edward A., Bomble, Yannick J., Brunecky, Roman, Chen, Xiaowen, Felby, Claus, Jeoh, Tina, Kumar, Rajeev, McCleary, Barry V., Pletschke, Brett I., Tucker, Melvin P., Wyman, Charles E., and Decker, Stephen R. Undefined cellulase formulations hinder scientific reproducibility. Netherlands: N. p., 2017. Web. doi:10.1186/s13068-017-0974-y.
Himmel, Michael E., Abbas, Charles A., Baker, John O., Bayer, Edward A., Bomble, Yannick J., Brunecky, Roman, Chen, Xiaowen, Felby, Claus, Jeoh, Tina, Kumar, Rajeev, McCleary, Barry V., Pletschke, Brett I., Tucker, Melvin P., Wyman, Charles E., & Decker, Stephen R. Undefined cellulase formulations hinder scientific reproducibility. Netherlands. doi:10.1186/s13068-017-0974-y.
Himmel, Michael E., Abbas, Charles A., Baker, John O., Bayer, Edward A., Bomble, Yannick J., Brunecky, Roman, Chen, Xiaowen, Felby, Claus, Jeoh, Tina, Kumar, Rajeev, McCleary, Barry V., Pletschke, Brett I., Tucker, Melvin P., Wyman, Charles E., and Decker, Stephen R. Tue . "Undefined cellulase formulations hinder scientific reproducibility". Netherlands. doi:10.1186/s13068-017-0974-y.
@article{osti_1618705,
title = {Undefined cellulase formulations hinder scientific reproducibility},
author = {Himmel, Michael E. and Abbas, Charles A. and Baker, John O. and Bayer, Edward A. and Bomble, Yannick J. and Brunecky, Roman and Chen, Xiaowen and Felby, Claus and Jeoh, Tina and Kumar, Rajeev and McCleary, Barry V. and Pletschke, Brett I. and Tucker, Melvin P. and Wyman, Charles E. and Decker, Stephen R.},
abstractNote = {In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel production. For the effective comparison of one cellulase preparation to another, cellulase assays are typically carried out with one or more engineered cellulase formulations or natural exoproteomes of known performance serving as positive controls. When these formulations have unknown composition, as is the case with several widely used commercial products, it becomes impossible to compare or reproduce work done today to work done in the future, where, for example, such preparations may not be available. Therefore, being a critical tenet of science publishing, experimental reproducibility is endangered by the continued use of these undisclosed products. We propose the introduction of standard procedures and materials to produce specific and reproducible cellulase formulations. These formulations are to serve as yardsticks to measure improvements and performance of new cellulase formulations.},
doi = {10.1186/s13068-017-0974-y},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {Netherlands},
year = {2017},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s13068-017-0974-y

Citation Metrics:
Cited by: 1 work
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    Effects of cre1 modification in the white-rot fungus Pleurotus ostreatus PC9: altering substrate preference during biological pretreatment
    journal, July 2018


    Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance
    journal, April 2019