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Title: Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria

Abstract

With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20–31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenting organisms to produce acetate at the expense of ethanol. Hydrogen and ethanol are both more reduced than glucose. Thus there is a tradeoff between production of these compounds imposed by electron balancing requirements; however, the mechanism is not fully known. Deletion of the hfsA or B subunits resulted in a roughly 1.8-fold increase in ethanol yield. The increase in ethanol production appears to be associated with an increase in alcohol dehydrogenase activity, which appears to be due, at least in part, to increased expression of the adhE gene, and may suggest a regulatory linkage between hfsB and adhE. We studied this system most intensively in the organism Thermoanaerobacterium saccharolyticum; however, deletion of hfsB also increases ethanol production in other thermophilic bacteria suggesting that this could be used as a general technique for engineering thermophilic bacteria for improved ethanol production in organisms with hfs-type hydrogenases. Since its discovery by Shaw et al. (JAMA 191:6457–64, 2009), the hfs hydrogenase has been suspected to act as a regulator duemore » to the presence of a PAS domain. We provide additional support for the presence of a regulatory phenomenon. In addition, we find a practical application for this scientific insight, namely increasing ethanol yield in strains that are of interest for ethanol production from cellulose or hemicellulose. In two of these organisms (T. xylanolyticum and T. thermosaccharolyticum), the ethanol yields are the highest reported to date.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dartmouth College, Hanover, NH (United States); Recep Tayyip Erdogan Univ., Rize (Turkey)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Scientific and Technological Research Council of Turkey
OSTI Identifier:
1618704
Alternate Identifier(s):
OSTI ID: 1435216
Grant/Contract Number:  
AC05-00OR22725; AC02- 05CH11231; AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Thermoanaerobacterium saccharolyticum; Clostridium thermocellum; Thermoanaerobacter mathranii; Thermoanaerobacterium thermosaccharolyticum; Thermoanaerobacterium xylanolyticum; hydrogenase; ethanol

Citation Formats

Eminoğlu, Ayşenur, Murphy, Sean Jean-Loup, Maloney, Marybeth, Lanahan, Anthony, Giannone, Richard J., Hettich, Robert L., Tripathi, Shital A., Beldüz, Ali Osman, Lynd, Lee R., and Olson, Daniel G. Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria. Netherlands: N. p., 2017. Web. doi:10.1186/s13068-017-0968-9.
Eminoğlu, Ayşenur, Murphy, Sean Jean-Loup, Maloney, Marybeth, Lanahan, Anthony, Giannone, Richard J., Hettich, Robert L., Tripathi, Shital A., Beldüz, Ali Osman, Lynd, Lee R., & Olson, Daniel G. Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria. Netherlands. doi:10.1186/s13068-017-0968-9.
Eminoğlu, Ayşenur, Murphy, Sean Jean-Loup, Maloney, Marybeth, Lanahan, Anthony, Giannone, Richard J., Hettich, Robert L., Tripathi, Shital A., Beldüz, Ali Osman, Lynd, Lee R., and Olson, Daniel G. Thu . "Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria". Netherlands. doi:10.1186/s13068-017-0968-9.
@article{osti_1618704,
title = {Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria},
author = {Eminoğlu, Ayşenur and Murphy, Sean Jean-Loup and Maloney, Marybeth and Lanahan, Anthony and Giannone, Richard J. and Hettich, Robert L. and Tripathi, Shital A. and Beldüz, Ali Osman and Lynd, Lee R. and Olson, Daniel G.},
abstractNote = {With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20–31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenting organisms to produce acetate at the expense of ethanol. Hydrogen and ethanol are both more reduced than glucose. Thus there is a tradeoff between production of these compounds imposed by electron balancing requirements; however, the mechanism is not fully known. Deletion of the hfsA or B subunits resulted in a roughly 1.8-fold increase in ethanol yield. The increase in ethanol production appears to be associated with an increase in alcohol dehydrogenase activity, which appears to be due, at least in part, to increased expression of the adhE gene, and may suggest a regulatory linkage between hfsB and adhE. We studied this system most intensively in the organism Thermoanaerobacterium saccharolyticum; however, deletion of hfsB also increases ethanol production in other thermophilic bacteria suggesting that this could be used as a general technique for engineering thermophilic bacteria for improved ethanol production in organisms with hfs-type hydrogenases. Since its discovery by Shaw et al. (JAMA 191:6457–64, 2009), the hfs hydrogenase has been suspected to act as a regulator due to the presence of a PAS domain. We provide additional support for the presence of a regulatory phenomenon. In addition, we find a practical application for this scientific insight, namely increasing ethanol yield in strains that are of interest for ethanol production from cellulose or hemicellulose. In two of these organisms (T. xylanolyticum and T. thermosaccharolyticum), the ethanol yields are the highest reported to date.},
doi = {10.1186/s13068-017-0968-9},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {Netherlands},
year = {2017},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s13068-017-0968-9

Citation Metrics:
Cited by: 3 works
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Figures / Tables:

Fig. 1 Fig. 1: Comparison of fermentation products produced by strains of T. saccharolyticum with mutations in the hfs operon. Strains were grown in MTC‑6 medium on 5 g/l (14.7 mM) cellobiose. Error bars represent one standard deviation, n ≥ 3

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    Works referencing / citing this record:

    Expression of benzoyl-CoA metabolism genes in the lignocellulolytic host Caldicellulosiruptor bescii
    journal, May 2019


      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.