Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

Biswal, Ajaya K.; Tan, Li; Atmodjo, Melani A.; DeMartini, Jaclyn; Gelineo-Albersheim, Ivana; Hunt, Kimberly; Black, Ian M.; Mohanty, Sushree S.; Ryno, David; Wyman, Charles E.; Mohnen, Debra

doi:10.1186/s13068-017-0866-1

Title: Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

Journal Article · 14 July 2017 · Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-017-0866-1 · OSTI ID:1618682

Biswal, Ajaya K. ^[1]; Tan, Li ^[1]; Atmodjo, Melani A. ^[1]; DeMartini, Jaclyn ^[2]; Gelineo-Albersheim, Ivana ^[3]; Hunt, Kimberly ^[3]; Black, Ian M. ^[4]; Mohanty, Sushree S. ^[3]; Ryno, David ^[3]; Wyman, Charles E. ^[2]; Mohnen, Debra ^[1]

University of Georgia, Athens, GA (United States). Department of Biochemistry and Molecular Biology; University of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; DOE-BioEnergy Science Center (BESC), Oak Ridge, TN (United States)
DOE-BioEnergy Science Center (BESC), Oak Ridge, TN (United States); University of California Riverside, California (United States). Center for Environmental Research and Technology (CE-CERT) and Department of Chemical and Environmental Engineering
University of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; DOE-BioEnergy Science Center (BESC), Oak Ridge, TN (United States)
University of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

The effective use of plant biomass for biofuel and bioproduct production requires a comprehensive glycosyl residue composition analysis to understand the different cell wall polysaccharides present in the different biomass sources. Here we compared four methods side-by-side for their ability to measure the neutral and acidic sugar composition of cell walls from herbaceous, grass, and woody model plants and bioenergy feedstocks.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); USDOE

Grant/Contract Number:: SC0015662; PS02-06ER64304

OSTI ID:: 1618682

Alternate ID(s):: OSTI ID: 1626983

Journal Information:: Biotechnology for Biofuels, Vol. 10, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 15 works

Citation information provided by
Web of Science

References (92)

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production Himmel, M. E.; Ding, S.-Y.; Johnson, D. K. Science, Vol. 315, Issue 5813, p. 804-807 https://doi.org/10.1126/science.1137016	journal	February 2007
Comparative efficiency and driving range of light- and heavy-duty vehicles powered with biomass energy stored in liquid fuels or batteries Laser, Mark; Lynd, Lee R. Proceedings of the National Academy of Sciences, Vol. 111, Issue 9 https://doi.org/10.1073/pnas.1314039111	journal	February 2014
Meeting the Sustainable Development Goals leads to lower world population growth Abel, Guy J.; Barakat, Bilal; Kc, Samir Proceedings of the National Academy of Sciences, Vol. 113, Issue 50 https://doi.org/10.1073/pnas.1611386113	journal	November 2016
The cellulose resource matrix Keijsers, Edwin R. P.; Yılmaz, Gülden; van Dam, Jan E. G. Carbohydrate Polymers, Vol. 93, Issue 1 https://doi.org/10.1016/j.carbpol.2012.08.110	journal	March 2013
Isolation and characterization of plant cell walls and cell wall components York, William S.; Darvill, Alan G.; McNeil, Michael Methods in Enzymology https://doi.org/10.1016/0076-6879(86)18062-1	book	January 1986
A simple and rapid preparation of alditol acetates for monosaccharide analysis Blakeney, Anthony B.; Harris, Philip J.; Henry, Robert J. Carbohydrate Research, Vol. 113, Issue 2 https://doi.org/10.1016/0008-6215(83)88244-5	journal	March 1983
Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway Fujikura, Ushio; Elsaesser, Lore; Breuninger, Holger PLoS Genetics, Vol. 10, Issue 9 https://doi.org/10.1371/journal.pgen.1004627	journal	September 2014
Use of the alditol acetate derivatisation for the analysis of reducing sugars in potato tubers Brunton, N. P.; Gormley, T. R.; Murray, B. Food Chemistry, Vol. 104, Issue 1 https://doi.org/10.1016/j.foodchem.2007.01.045	journal	January 2007
Changes in cell wall polysaccharides in developing barley (Hordeum vulgare) coleoptiles Gibeaut, David M.; Pauly, Markus; Bacic, Antony Planta, Vol. 221, Issue 5 https://doi.org/10.1007/s00425-005-1481-0	journal	April 2005
Structural characterisation of xyloglucan secreted by suspension-cultured cells of Nicotiana plumbaginifolia Sims, Ian M.; Munro, Sharon L. A.; Currie, Graeme Carbohydrate Research, Vol. 293, Issue 2 https://doi.org/10.1016/0008-6215(96)00142-5	journal	October 1996
Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield Biswal, Ajaya K.; Soeno, Kazuo; Gandla, Madhavi Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-11	journal	January 2014
BRITTLE SHEATH1 encoding OsCYP96B4 is involved in secondary cell wall formation in rice Wang, Xiaole; Cheng, Zhijun; Zhao, Zhichao Plant Cell Reports, Vol. 35, Issue 4 https://doi.org/10.1007/s00299-015-1916-4	journal	December 2015
Structural analysis of arabinoxylans isolated from ball-milled switchgrass biomass Mazumder, Koushik; York, William S. Carbohydrate Research, Vol. 345, Issue 15 https://doi.org/10.1016/j.carres.2010.07.034	journal	October 2010
An Arabidopsis Cell Wall Proteoglycan Consists of Pectin and Arabinoxylan Covalently Linked to an Arabinogalactan Protein Tan, Li; Eberhard, Stefan; Pattathil, Sivakumar The Plant Cell, Vol. 25, Issue 1 https://doi.org/10.1105/tpc.112.107334	journal	January 2013
Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth: Rice cellulose synthase-like D4 is essential for normal cell-wall Li, Ming; Xiong, Guangyan; Li, Rui The Plant Journal, Vol. 60, Issue 6 https://doi.org/10.1111/j.1365-313X.2009.04022.x	journal	December 2009
Determination of the neutral and acidic glycosyl-residue compositions of plant polysaccharides by GC-EI-MS analysis of the trimethylsilyl methyl glycoside derivatives Doco, T.; O'Neill, M. A.; Pellerin, P. Carbohydrate Polymers, Vol. 46, Issue 3 https://doi.org/10.1016/S0144-8617(00)00328-3	journal	November 2001
Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock Biswal, Ajaya K.; Hao, Zhangying; Pattathil, Sivakumar Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0218-y	journal	January 2015
Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion Lionetti, V.; Francocci, F.; Ferrari, S. Proceedings of the National Academy of Sciences, Vol. 107, Issue 2 https://doi.org/10.1073/pnas.0907549107	journal	December 2009
Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants Petersen, Pia Damm; Lau, Jane; Ebert, Berit Biotechnology for Biofuels, Vol. 5, Issue 1, Article No. 84 https://doi.org/10.1186/1754-6834-5-84	journal	January 2012
Heterogeneity in the chemistry, structure and function of plant cell walls Burton, Rachel A.; Gidley, Michael J.; Fincher, Geoffrey B. Nature Chemical Biology, Vol. 6, Issue 10 https://doi.org/10.1038/nchembio.439	journal	September 2010
Changes in Esterification of the Uronic Acid Groups of Cell Wall Polysaccharides during Elongation of Maize Coleoptiles Kim, Jong-Bum; Carpita, Nicholas C. Plant Physiology, Vol. 98, Issue 2 https://doi.org/10.1104/pp.98.2.646	journal	February 1992
Loss of Highly Branched Arabinans and Debranching of Rhamnogalacturonan I Accompany Loss of Firm Texture and Cell Separation during Prolonged Storage of Apple Peña, María J.; Carpita, Nicholas C. Plant Physiology, Vol. 135, Issue 3 https://doi.org/10.1104/pp.104.043679	journal	July 2004
Resolution of complex monosaccharide mixtures from plant cell wall isolates by high pH anion exchange chromatography Currie, Heather A.; Perry, Carole C. Journal of Chromatography A, Vol. 1128, Issue 1-2 https://doi.org/10.1016/j.chroma.2006.06.045	journal	September 2006
CarboPac™ PA20: a new monosaccharide separator column with electrochemical detection with disposable gold electrodes Weitzhandler, Michael; Barreto, Victor; Pohl, Christopher Journal of Biochemical and Biophysical Methods, Vol. 60, Issue 3 https://doi.org/10.1016/j.jbbm.2004.01.009	journal	September 2004
Arabidopsis leucine-rich repeat extensin (LRX) proteins modify cell wall composition and influence plant growth Draeger, Christian; Ndinyanka Fabrice, Tohnyui; Gineau, Emilie BMC Plant Biology, Vol. 15, Issue 1 https://doi.org/10.1186/s12870-015-0548-8	journal	June 2015
Dynamic changes in cell wall polysaccharides during wheat seedling development Obel, N. Phytochemistry, Vol. 60, Issue 6 https://doi.org/10.1016/S0031-9422(02)00148-6	journal	July 2002
Rhamnogalacturonan I in Solanum tuberosum tubers contains complex arabinogalactan structures Øbro, Jens; Harholt, Jesper; Scheller, Henrik Vibe Phytochemistry, Vol. 65, Issue 10 https://doi.org/10.1016/j.phytochem.2004.05.002	journal	May 2004
XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan Chiniquy, D.; Sharma, V.; Schultink, A. Proceedings of the National Academy of Sciences, Vol. 109, Issue 42 https://doi.org/10.1073/pnas.1202079109	journal	October 2012
Arabidopsis thaliana T-DNA Mutants Implicate GAUT Genes in the Biosynthesis of Pectin and Xylan in Cell Walls and Seed Testa Caffall, Kerry H.; Pattathil, Sivakumar; Phillips, Sarah E. Molecular Plant, Vol. 2, Issue 5 https://doi.org/10.1093/mp/ssp062	journal	September 2009
Biolistic transformation of elite genotypes of switchgrass (Panicum virgatum L.) King, Zachary R.; Bray, Adam L.; LaFayette, Peter R. Plant Cell Reports, Vol. 33, Issue 2 https://doi.org/10.1007/s00299-013-1531-1	journal	November 2013
Describing and Quantifying Growth Stages of Perennial Forage Grasses Moore, K. J.; Moser, L. E.; Vogel, K. P. Agronomy Journal, Vol. 83, Issue 6 https://doi.org/10.2134/agronj1991.00021962008300060027x	journal	January 1991
A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography Albersheim, Peter; Nevins, Donald J.; English, Patricia D. Carbohydrate Research, Vol. 5, Issue 3 https://doi.org/10.1016/S0008-6215(00)80510-8	journal	November 1967
New method for quantitative determination of uronic acids Blumenkrantz, Nelly; Asboe-Hansen, Gustav Analytical Biochemistry, Vol. 54, Issue 2, p. 484-489 https://doi.org/10.1016/0003-2697(73)90377-1	journal	August 1973
Measurement of uronic acids without interference from neutral sugars Filisetti-Cozzi, Tullia M. C. C.; Carpita, Nicholas C. Analytical Biochemistry, Vol. 197, Issue 1 https://doi.org/10.1016/0003-2697(91)90372-Z	journal	August 1991
A Microtiter Plate Assay for the Determination of Uronic Acids van den Hoogen, Bianca M.; van Weeren, P. René; Lopes-Cardozo, Matthijs Analytical Biochemistry, Vol. 257, Issue 2 https://doi.org/10.1006/abio.1997.2538	journal	March 1998
Development of complete hydrolysis of pectins from apple pomace Wikiera, Agnieszka; Mika, Magdalena; Starzyńska-Janiszewska, Anna Food Chemistry, Vol. 172 https://doi.org/10.1016/j.foodchem.2014.09.132	journal	April 2015
Stoichiometric depolymerization of polyuronides and glycosaminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl group Taylor, R. L.; Conrad, H. E. Biochemistry, Vol. 11, Issue 8 https://doi.org/10.1021/bi00758a009	journal	April 1972
Determination of the degree of methyl esterification of pectins in small samples by selective reduction of esterified galacturonic acid to galactose Maness, Niels O.; Ryan, James D.; Mort, Andrew J. Analytical Biochemistry, Vol. 185, Issue 2 https://doi.org/10.1016/0003-2697(90)90306-T	journal	March 1990
[1] Carbohydrate composition analysis of glycoconjugates by gas-liquid chromatography/mass spectrometry Merkle, Roberta K.; Poppe, Izabella Methods in Enzymology https://doi.org/10.1016/0076-6879(94)30003-8	book	January 1994
Screening of Arabidopsis thaliana stems for variation in cell wall polysaccharides Gardner, S.; Burrell, M.; Fry, S. Phytochemistry, Vol. 60, Issue 3 https://doi.org/10.1016/S0031-9422(02)00046-8	journal	June 2002
Unique aspects of the grass cell wall Vogel, J. Current Opinion in Plant Biology, Vol. 11, Issue 3 https://doi.org/10.1016/j.pbi.2008.03.002	journal	June 2008
Cell-wall carbohydrates and their modification as a resource for biofuels Pauly, Markus; Keegstra, Kenneth The Plant Journal, Vol. 54, Issue 4, p. 559-568 https://doi.org/10.1111/j.1365-313X.2008.03463.x	journal	May 2008
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth Carpita, Nicholas C.; Gibeaut, David M. The Plant Journal, Vol. 3, Issue 1 https://doi.org/10.1111/j.1365-313X.1993.tb00007.x	journal	January 1993
Rapid Assessment of Lignin Content and Structure in Switchgrass (Panicum virgatum L.) Grown Under Different Environmental Conditions Mann, David G. J.; Labbé, Nicole; Sykes, Robert W. BioEnergy Research, Vol. 2, Issue 4 https://doi.org/10.1007/s12155-009-9054-x	journal	October 2009
Disrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall Component Cavalier, David M.; Lerouxel, Olivier; Neumetzler, Lutz The Plant Cell, Vol. 20, Issue 6 https://doi.org/10.1105/tpc.108.059873	journal	June 2008
An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14 Mortimer, Jenny C.; Faria-Blanc, Nuno; Yu, Xiaolan The Plant Journal, Vol. 83, Issue 3 https://doi.org/10.1111/tpj.12898	journal	June 2015
Structure and Biogenesis of the cell Walls of Grasses Carpita, Nicholas C. Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1 https://doi.org/10.1146/annurev.arplant.47.1.445	journal	June 1996
Revolutionary Times in Our Understanding of Cell Wall Biosynthesis and Remodeling in the Grasses Fincher, Geoffrey B. Plant Physiology, Vol. 149, Issue 1 https://doi.org/10.1104/pp.108.130096	journal	January 2009
Cell Wall Architecture of the Elongating Maize Coleoptile Carpita, Nicholas C.; Defernez, Marianne; Findlay, Kim Plant Physiology, Vol. 127, Issue 2 https://doi.org/10.1104/pp.010146	journal	October 2001
The reaction of carbazole with carbohydrates Galambos, John T. Analytical Biochemistry, Vol. 19, Issue 1 https://doi.org/10.1016/0003-2697(67)90141-8	journal	April 1967
Pectin Biosynthesis Is Critical for Cell Wall Integrity and Immunity in Arabidopsis thaliana Bethke, Gerit; Thao, Amanda; Xiong, Guangyan The Plant Cell, Vol. 28, Issue 2 https://doi.org/10.1105/tpc.15.00404	journal	January 2016
Analysis of pectin mutants and natural accessions of Arabidopsis highlights the impact of de-methyl-esterified homogalacturonan on tissue saccharification Francocci, Fedra; Bastianelli, Elisa; Lionetti, Vincenzo Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-163	journal	January 2013
Determining the polysaccharide composition of plant cell walls Pettolino, Filomena A.; Walsh, Cherie; Fincher, Geoffrey B. Nature Protocols, Vol. 7, Issue 9 https://doi.org/10.1038/nprot.2012.081	journal	August 2012
Analytical Methods in Wood Chemistry, Pulping, and Papermaking Sjöström, Eero; Alén, Raimo Springer Series in Wood Science https://doi.org/10.1007/978-3-662-03898-7	book	January 1999
Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? Villas-Bôas, Silas G.; Smart, Kathleen F.; Sivakumaran, Subathira Metabolites, Vol. 1, Issue 1 https://doi.org/10.3390/metabo1010003	journal	January 2011
Analysis of Phenolic and Cyclic Compounds in Plants Using Derivatization Techniques in Combination with GC-MS-Based Metabolite Profiling Rohloff, Jens Molecules, Vol. 20, Issue 2 https://doi.org/10.3390/molecules20023431	journal	February 2015
Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? Villas-Bôas, Silas G.; Smart, Kathleen F.; Sivakumaran, Subathira Metabolites, Vol. 1, Issue 1 https://doi.org/10.3390/metabo01010003	journal	January 2011
Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway Lore, Elsaesser,; Holger, Breuninger,; Clara, Sánchez-Rodríguez, ETH Zurich https://doi.org/10.3929/ethz-b-000184743	text	January 2014
Arabidopsis leucine-rich repeat extensin (LRX) proteins modify cell wall composition and influence plant growth Draeger, Christian; Ndinyanka Fabrice, Tohnyui; Gineau, Emilie BioMed Central https://doi.org/10.5167/uzh-121499	text	January 2015
The reaction of carbazole with carbohydrates Galambos, John T. Analytical Biochemistry, Vol. 19, Issue 1 https://doi.org/10.1016/0003-2697(67)90142-x	journal	April 1967
A Microtiter Plate Assay for the Determination of Uronic Acids van den Hoogen, Bianca M.; van Weeren, P. René; Lopes-Cardozo, Matthijs Analytical Biochemistry, Vol. 257, Issue 2 https://doi.org/10.1006/abio.1997.2538	journal	March 1998
Biolistic transformation of elite genotypes of switchgrass (Panicum virgatum L.) King, Zachary R.; Bray, Adam L.; LaFayette, Peter R. Plant Cell Reports, Vol. 33, Issue 2 https://doi.org/10.1007/s00299-013-1531-1	journal	November 2013
Rapid Assessment of Lignin Content and Structure in Switchgrass (Panicum virgatum L.) Grown Under Different Environmental Conditions Mann, David G. J.; Labbé, Nicole; Sykes, Robert W. BioEnergy Research, Vol. 2, Issue 4 https://doi.org/10.1007/s12155-009-9054-x	journal	October 2009
The reaction of carbazole with carbohydrates Galambos, John T. Analytical Biochemistry, Vol. 19, Issue 1 https://doi.org/10.1016/0003-2697(67)90141-8	journal	April 1967
New method for quantitative determination of uronic acids Blumenkrantz, Nelly; Asboe-Hansen, Gustav Analytical Biochemistry, Vol. 54, Issue 2, p. 484-489 https://doi.org/10.1016/0003-2697(73)90377-1	journal	August 1973
A simple and rapid preparation of alditol acetates for monosaccharide analysis Blakeney, Anthony B.; Harris, Philip J.; Henry, Robert J. Carbohydrate Research, Vol. 113, Issue 2 https://doi.org/10.1016/0008-6215(83)88244-5	journal	March 1983
[1] Carbohydrate composition analysis of glycoconjugates by gas-liquid chromatography/mass spectrometry Merkle, Roberta K.; Poppe, Izabella Methods in Enzymology https://doi.org/10.1016/0076-6879(94)30003-8	book	January 1994
Structural analysis of arabinoxylans isolated from ball-milled switchgrass biomass Mazumder, Koushik; York, William S. Carbohydrate Research, Vol. 345, Issue 15 https://doi.org/10.1016/j.carres.2010.07.034	journal	October 2010
Development of complete hydrolysis of pectins from apple pomace Wikiera, Agnieszka; Mika, Magdalena; Starzyńska-Janiszewska, Anna Food Chemistry, Vol. 172 https://doi.org/10.1016/j.foodchem.2014.09.132	journal	April 2015
CarboPac™ PA20: a new monosaccharide separator column with electrochemical detection with disposable gold electrodes Weitzhandler, Michael; Barreto, Victor; Pohl, Christopher Journal of Biochemical and Biophysical Methods, Vol. 60, Issue 3 https://doi.org/10.1016/j.jbbm.2004.01.009	journal	September 2004
Unique aspects of the grass cell wall Vogel, J. Current Opinion in Plant Biology, Vol. 11, Issue 3 https://doi.org/10.1016/j.pbi.2008.03.002	journal	June 2008
Rhamnogalacturonan I in Solanum tuberosum tubers contains complex arabinogalactan structures Øbro, Jens; Harholt, Jesper; Scheller, Henrik Vibe Phytochemistry, Vol. 65, Issue 10 https://doi.org/10.1016/j.phytochem.2004.05.002	journal	May 2004
Stoichiometric depolymerization of polyuronides and glycosaminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl group Taylor, R. L.; Conrad, H. E. Biochemistry, Vol. 11, Issue 8 https://doi.org/10.1021/bi00758a009	journal	April 1972
Heterogeneity in the chemistry, structure and function of plant cell walls Burton, Rachel A.; Gidley, Michael J.; Fincher, Geoffrey B. Nature Chemical Biology, Vol. 6, Issue 10 https://doi.org/10.1038/nchembio.439	journal	September 2010
Determining the polysaccharide composition of plant cell walls Pettolino, Filomena A.; Walsh, Cherie; Fincher, Geoffrey B. Nature Protocols, Vol. 7, Issue 9 https://doi.org/10.1038/nprot.2012.081	journal	August 2012
Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion Lionetti, V.; Francocci, F.; Ferrari, S. Proceedings of the National Academy of Sciences, Vol. 107, Issue 2 https://doi.org/10.1073/pnas.0907549107	journal	December 2009
XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan Chiniquy, D.; Sharma, V.; Schultink, A. Proceedings of the National Academy of Sciences, Vol. 109, Issue 42 https://doi.org/10.1073/pnas.1202079109	journal	October 2012
Arabidopsis thaliana T-DNA Mutants Implicate GAUT Genes in the Biosynthesis of Pectin and Xylan in Cell Walls and Seed Testa Caffall, Kerry H.; Pattathil, Sivakumar; Phillips, Sarah E. Molecular Plant, Vol. 2, Issue 5 https://doi.org/10.1093/mp/ssp062	journal	September 2009
Cell Wall Architecture of the Elongating Maize Coleoptile Carpita, Nicholas C.; Defernez, Marianne; Findlay, Kim Plant Physiology, Vol. 127, Issue 2 https://doi.org/10.1104/pp.010146	journal	October 2001
Revolutionary Times in Our Understanding of Cell Wall Biosynthesis and Remodeling in the Grasses Fincher, Geoffrey B. Plant Physiology, Vol. 149, Issue 1 https://doi.org/10.1104/pp.108.130096	journal	January 2009
Changes in Esterification of the Uronic Acid Groups of Cell Wall Polysaccharides during Elongation of Maize Coleoptiles Kim, Jong-Bum; Carpita, Nicholas C. Plant Physiology, Vol. 98, Issue 2 https://doi.org/10.1104/pp.98.2.646	journal	February 1992
An Arabidopsis Cell Wall Proteoglycan Consists of Pectin and Arabinoxylan Covalently Linked to an Arabinogalactan Protein Tan, Li; Eberhard, Stefan; Pattathil, Sivakumar The Plant Cell, Vol. 25, Issue 1 https://doi.org/10.1105/tpc.112.107334	journal	January 2013
Pectin Biosynthesis Is Critical for Cell Wall Integrity and Immunity in Arabidopsis thaliana Bethke, Gerit; Thao, Amanda; Xiong, Guangyan The Plant Cell, Vol. 28, Issue 2 https://doi.org/10.1105/tpc.15.00404	journal	January 2016
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production Himmel, M. E.; Ding, S.-Y.; Johnson, D. K. Science, Vol. 315, Issue 5813, p. 804-807 https://doi.org/10.1126/science.1137016	journal	February 2007
Structure and Biogenesis of the cell Walls of Grasses Carpita, Nicholas C. Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1 https://doi.org/10.1146/annurev.arplant.47.1.445	journal	June 1996
Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants Petersen, Pia Damm; Lau, Jane; Ebert, Berit Biotechnology for Biofuels, Vol. 5, Issue 1, Article No. 84 https://doi.org/10.1186/1754-6834-5-84	journal	January 2012
Analysis of pectin mutants and natural accessions of Arabidopsis highlights the impact of de-methyl-esterified homogalacturonan on tissue saccharification Francocci, Fedra; Bastianelli, Elisa; Lionetti, Vincenzo Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-163	journal	January 2013
Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock Biswal, Ajaya K.; Hao, Zhangying; Pattathil, Sivakumar Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0218-y	journal	January 2015
Describing and Quantifying Growth Stages of Perennial Forage Grasses Moore, K. J.; Moser, L. E.; Vogel, K. P. Agronomy Journal, Vol. 83, Issue 6 https://doi.org/10.2134/agronj1991.00021962008300060027x	journal	January 1991
Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? Villas-Bôas, Silas G.; Smart, Kathleen F.; Sivakumaran, Subathira Metabolites, Vol. 1, Issue 1 https://doi.org/10.3390/metabo1010003	journal	January 2011
Atkinesin-13A Modulates Cell-Wall Synthesis and Cell Expansion in Arabidopsis thaliana via the THESEUS1 Pathway Lore, Elsaesser,; Holger, Breuninger,; Clara, Sánchez-Rodríguez, ETH Zurich https://doi.org/10.3929/ethz-b-000184743	text	January 2014
Rice plants response to the disruption ofOsCSLD4gene Li, Rui; Xiong, Guangyan; Zhang, Baocai Plant Signaling & Behavior, Vol. 5, Issue 2 https://doi.org/10.4161/psb.5.2.10369	journal	February 2010

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Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus Biswal, Ajaya K.; Atmodjo, Melani A.; Pattathil, Sivakumar Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-017-1002-y	journal	January 2018

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Biotechnology & Applied Microbiology
Energy & Fuels

Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747.v1 The current record is supplemented by this collection	collection	January 2017
MOESM1 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d1 The current record is supplemented by this text	text	January 2017
MOESM1 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d1.v1 The current record is supplemented by this text	text	January 2017
MOESM2 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d2 The current record is supplemented by this text	text	January 2017
MOESM2 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d2.v1 The current record is supplemented by this text	text	January 2017
MOESM3 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d3 The current record is supplemented by this text	text	January 2017
MOESM3 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d3.v1 The current record is supplemented by this text	text	January 2017
MOESM4 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d4 The current record is supplemented by this text	text	January 2017
MOESM4 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d4.v1 The current record is supplemented by this text	text	January 2017
MOESM5 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/M9.FIGSHARE.C.3826747_D5 The current record is supplemented by this dataset	dataset	January 2017
MOESM5 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d5.v1 The current record is supplemented by this dataset	dataset	January 2017
MOESM6 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/M9.FIGSHARE.C.3826747_D6 The current record is supplemented by this dataset	dataset	January 2017
MOESM6 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d6.v1 The current record is supplemented by this dataset	dataset	January 2017
MOESM7 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d7 The current record is supplemented by this text	text	January 2017
MOESM7 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues Biswal, Ajaya; Tan, Li; Atmodjo, Melani https://doi.org/10.6084/m9.figshare.c.3826747_d7.v1 The current record is supplemented by this text	text	January 2017