Improved sugar yields from biomass sorghum feedstocks: comparing low-lignin mutants and pretreatment chemistries
Abstract
For biofuel production processes to be economically efficient, it is essential to maximize the production of monomeric carbohydrates from the structural carbohydrates of feedstocks. One strategy for maximizing carbohydrate production is to identify less recalcitrant feedstock cultivars by performing some type of experimental screening on a large and diverse set of candidate materials, or by identifying genetic modifications (random or directed mutations or transgenic plants) that provide decreased recalcitrance. Economic efficiency can also be increased using additional pretreatment processes such as deacetylation, which uses dilute NaOH to remove the acetyl groups of hemicellulose prior to dilute acid pretreatment. In this work, we used a laboratory-scale screening tool that mimics relevant thermochemical pretreatment conditions to compare the total sugar yield of three near-isogenic brown midrib (bmr) mutant lines and the wild-type (WT) sorghum cultivar. We then compared results obtained from the laboratory-scale screening pretreatment assay to a large-scale pretreatment system. After pretreatment and enzymatic hydrolysis, the bmr mutants had higher total sugar yields than the WT sorghum cultivar. Increased pretreatment temperatures increased reactivity for all sorghum samples reducing the differences observed at lower reaction temperatures. Deacetylation prior to dilute acid pretreatment increased the total sugar yield for all four sorghum samples,more »
- Authors:
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO); Walloon Agricultural Research Center, Belgium
- OSTI Identifier:
- 1618656
- Alternate Identifier(s):
- OSTI ID: 1335583
- Report Number(s):
- NREL/JA-5100-67543
Journal ID: ISSN 1754-6834; 251; PII: 667
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; sorghum bicolor; brown midrib; pretreatment; total sugar yield; biofuels
Citation Formats
Godin, Bruno, Nagle, Nick, Sattler, Scott, Agneessens, Richard, Delcarte, Jérôme, and Wolfrum, Edward. Improved sugar yields from biomass sorghum feedstocks: comparing low-lignin mutants and pretreatment chemistries. Netherlands: N. p., 2016.
Web. doi:10.1186/s13068-016-0667-y.
Godin, Bruno, Nagle, Nick, Sattler, Scott, Agneessens, Richard, Delcarte, Jérôme, & Wolfrum, Edward. Improved sugar yields from biomass sorghum feedstocks: comparing low-lignin mutants and pretreatment chemistries. Netherlands. https://doi.org/10.1186/s13068-016-0667-y
Godin, Bruno, Nagle, Nick, Sattler, Scott, Agneessens, Richard, Delcarte, Jérôme, and Wolfrum, Edward. Mon .
"Improved sugar yields from biomass sorghum feedstocks: comparing low-lignin mutants and pretreatment chemistries". Netherlands. https://doi.org/10.1186/s13068-016-0667-y.
@article{osti_1618656,
title = {Improved sugar yields from biomass sorghum feedstocks: comparing low-lignin mutants and pretreatment chemistries},
author = {Godin, Bruno and Nagle, Nick and Sattler, Scott and Agneessens, Richard and Delcarte, Jérôme and Wolfrum, Edward},
abstractNote = {For biofuel production processes to be economically efficient, it is essential to maximize the production of monomeric carbohydrates from the structural carbohydrates of feedstocks. One strategy for maximizing carbohydrate production is to identify less recalcitrant feedstock cultivars by performing some type of experimental screening on a large and diverse set of candidate materials, or by identifying genetic modifications (random or directed mutations or transgenic plants) that provide decreased recalcitrance. Economic efficiency can also be increased using additional pretreatment processes such as deacetylation, which uses dilute NaOH to remove the acetyl groups of hemicellulose prior to dilute acid pretreatment. In this work, we used a laboratory-scale screening tool that mimics relevant thermochemical pretreatment conditions to compare the total sugar yield of three near-isogenic brown midrib (bmr) mutant lines and the wild-type (WT) sorghum cultivar. We then compared results obtained from the laboratory-scale screening pretreatment assay to a large-scale pretreatment system. After pretreatment and enzymatic hydrolysis, the bmr mutants had higher total sugar yields than the WT sorghum cultivar. Increased pretreatment temperatures increased reactivity for all sorghum samples reducing the differences observed at lower reaction temperatures. Deacetylation prior to dilute acid pretreatment increased the total sugar yield for all four sorghum samples, and reduced the differences in total sugar yields among them, but solubilized a sizable fraction of the non-structural carbohydrates. The general trends of increased total sugar yield in the bmr mutant compared to the WT seen at the laboratory scale were observed at the large-scale system. However, in the larger reactor system, the measured total sugar yields were lower and the difference in total sugar yield between the WT and bmr sorghum was larger. Sorghum bmr mutants, which have a reduced lignin content showed higher total sugar yields than the WT cultivar after dilute acid pretreatment and enzymatic hydrolysis. In conclusion, deacetylation prior to dilute acid pretreatment increased the total sugar yield for all four sorghum samples. However, since deacetylation also solubilizes a large fraction of the non-structural carbohydrates, the ability to derive value from these solubilized sugars will depend greatly on the proposed conversion process.},
doi = {10.1186/s13068-016-0667-y},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {Netherlands},
year = {Mon Nov 21 00:00:00 EST 2016},
month = {Mon Nov 21 00:00:00 EST 2016}
}
https://doi.org/10.1186/s13068-016-0667-y
Web of Science
Works referenced in this record:
A laboratory-scale pretreatment and hydrolysis assay for determination of reactivity in cellulosic biomass feedstocks
journal, January 2013
- Wolfrum, Edward J.; Ness, Ryan M.; Nagle, Nicholas J.
- Biotechnology for Biofuels, Vol. 6, Issue 1
Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum : Characterization of novel sorghum
journal, October 2015
- Scully, Erin D.; Gries, Tammy; Funnell-Harris, Deanna L.
- Journal of Integrative Plant Biology, Vol. 58, Issue 2
Lignin in plant biomasses: comparative metrological assessment of the detergent fiber and the insoluble dietary fiber methods
journal, May 2015
- Godin, Bruno; Agneessens, Richard; Gerin, Patrick
- Cellulose, Vol. 22, Issue 4
Registration of BN611, AN612, BN612, and RN613 Sorghum Genetic Stocks with Stacked -6 and -12 Genes
journal, January 2008
- Pedersen, J. F.; Toy, J. J.; Funnell, D. L.
- Journal of Plant Registrations, Vol. 2, Issue 3
‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies
journal, June 2009
- da Costa Sousa, Leonardo; Chundawat, Shishir PS; Balan, Venkatesh
- Current Opinion in Biotechnology, Vol. 20, Issue 3
Identification and Characterization of Four Missense Mutations in Brown midrib 12 (Bmr12), the Caffeic O-Methyltranferase (COMT) of Sorghum
journal, April 2012
- Sattler, Scott E.; Palmer, Nathan A.; Saballos, Ana
- BioEnergy Research, Vol. 5, Issue 4
Effect of acetylation/deacetylation on enzymatic hydrolysis of corn stalk
journal, December 2014
- Jiang, Wei; Peng, Huadong; Li, Hongqiang
- Biomass and Bioenergy, Vol. 71
A Nonsense Mutation in a Cinnamyl Alcohol Dehydrogenase Gene Is Responsible for the Sorghum brown midrib6 Phenotype
journal, April 2009
- Sattler, Scott E.; Saathoff, Aaron J.; Haas, Eric J.
- Plant Physiology, Vol. 150, Issue 2
Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents
journal, August 2009
- Dien, Bruce S.; Sarath, Gautam; Pedersen, Jeffrey F.
- BioEnergy Research, Vol. 2, Issue 3
Characterization of pilot-scale dilute acid pretreatment performance using deacetylated corn stover
journal, January 2014
- Shekiro III, Joseph; Kuhn, Erik M.; Nagle, Nicholas J.
- Biotechnology for Biofuels, Vol. 7, Issue 1
Evaluation and Characterization of Forage Sorghum as Feedstock for Fermentable Sugar Production
journal, August 2008
- Corredor, D. Y.; Salazar, J. M.; Hohn, K. L.
- Applied Biochemistry and Biotechnology, Vol. 158, Issue 1
The impacts of deacetylation prior to dilute acid pretreatment on the bioethanol process
journal, January 2012
- Chen, Xiaowen; Shekiro, Joseph; Franden, Mary Ann
- Biotechnology for Biofuels, Vol. 5, Issue 1
Biomass recalcitrance. Part II: Fundamentals of different pre-treatments to increase the enzymatic digestibility of lignocellulose: Part II: Fundamentals of different pre-treatments to increase the enzymatic digestibility of lignocellulose
journal, June 2012
- Zhao, Xuebing; Zhang, Lihua; Liu, Dehua
- Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 5
Chemical characteristics and biofuels potentials of various plant biomasses: influence of the harvesting date: Chemical characteristics and biofuels potentials of plant biomasses
journal, May 2013
- Godin, Bruno; Lamaudière, Stéphane; Agneessens, Richard
- Journal of the Science of Food and Agriculture, Vol. 93, Issue 13
Structural Carbohydrates in a Plant Biomass: Correlations between the Detergent Fiber and Dietary Fiber Methods
journal, June 2014
- Godin, Bruno; Agneessens, Richard; Gerin, Patrick
- Journal of Agricultural and Food Chemistry, Vol. 62, Issue 24
Process and technoeconomic analysis of leading pretreatment technologies for lignocellulosic ethanol production using switchgrass
journal, December 2011
- Tao, Ling; Aden, Andy; Elander, Richard T.
- Bioresource Technology, Vol. 102, Issue 24, p. 11105-11114
Registration of Twelve Grain Sorghum Genetic Stocks Near-isogenic for the Brown Midrib Genes -6 and -12
journal, January 2006
- Pedersen, J. F.; Funnell, D. L.; Toy, J. J.
- Crop Science, Vol. 46, Issue 1
Efficacy of Singular and Stacked brown midrib 6 and 12 in the Modification of Lignocellulose and Grain Chemistry
journal, March 2010
- Sattler, Scott E.; Funnell-Harris, Deanna L.; Pedersen, Jeffrey F.
- Journal of Agricultural and Food Chemistry, Vol. 58, Issue 6
Comparative analysis of efficiency, environmental impact, and process economics for mature biomass refining scenarios
journal, March 2009
- Laser, Mark; Larson, Eric; Dale, Bruce
- Biofuels, Bioproducts and Biorefining, Vol. 3, Issue 2
Compositional Analysis of Lignocellulosic Feedstocks. 2. Method Uncertainties
journal, August 2010
- Templeton, David W.; Scarlata, Christopher J.; Sluiter, Justin B.
- Journal of Agricultural and Food Chemistry, Vol. 58, Issue 16
Correlating detergent fiber analysis and dietary fiber analysis data for corn stover collected by NIRS
journal, June 2009
- Wolfrum, Edward J.; Lorenz, Aaron J.; deLeon, Natalia
- Cellulose, Vol. 16, Issue 4
High Xylose Yields from Dilute Acid Pretreatment of Corn Stover Under Process-Relevant Conditions
journal, January 2009
- Weiss, Noah D.; Nagle, Nicholas J.; Tucker, Melvin P.
- Applied Biochemistry and Biotechnology, Vol. 155, Issue 1-3
Chemical Composition and Biofuel Potentials of a Wide Diversity of Plant Biomasses
journal, April 2013
- Godin, Bruno; Lamaudière, Stéphane; Agneessens, Richard
- Energy & Fuels, Vol. 27, Issue 5
The use of an enzymatic technique to predict digestibility, metabolizable and net energy of compound feedstuffs for ruminants
journal, May 1986
- de Boever, J. L.; Cottyn, B. G.; Buysse, F. X.
- Animal Feed Science and Technology, Vol. 14, Issue 3-4
Biochemical methane potential prediction of plant biomasses: Comparing chemical composition versus near infrared methods and linear versus non-linear models
journal, January 2015
- Godin, Bruno; Mayer, Frédéric; Agneessens, Richard
- Bioresource Technology, Vol. 175
A Genomewide Analysis of the Cinnamyl Alcohol Dehydrogenase Family in Sorghum [ Sorghum bicolor (L.) Moench] Identifies SbCAD2 as the Brown midrib6 Gene
journal, December 2008
- Saballos, Ana; Ejeta, Gebisa; Sanchez, Emiliano
- Genetics, Vol. 181, Issue 2
Comparative Effects of the Sorghum -6 and -12 Genes
journal, January 2005
- Oliver, A. L.; Pedersen, J. F.; Grant, R. J.
- Crop Science, Vol. 45, Issue 6