PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus
Abstract
Background: Plant secondary cell wall is a renewable feedstock for biofuels and biomaterials production. Arabidopsis VASCULAR-RELATED NAC DOMAIN (VND) has been demonstrated to be a key transcription factor regulating secondary cell wall biosynthesis. However, less is known about its role in the woody species.Results: Here we report the functional characterization of Populus deltoides WOOD-ASSOCIATED NAC DOMAIN protein 3 (PdWND3A), a sequence homolog of Arabidopsis VND4 and VND5 that are members of transcription factor networks regulating secondary cell wall biosynthesis. PdWND3A was expressed at higher level in the xylem than in other tissues. The stem tissues of transgenic P. deltoides overexpressing PdWND3A (OXPdWND3A) contained more vessel cells than that of wild-type plants. Furthermore, lignin content and lignin monomer syringyl and guaiacyl (S/G) ratio were higher in OXPdWND3A transgenic plants than in wild-type plants. Consistent with these observations, the expression of FERULATE 5-HYDROXYLASE1 (F5H1), encoding an enzyme involved in the biosynthesis of sinapyl alcohol (S unit monolignol), was elevated in OXPdWND3A transgenic plants. Saccharification analysis indicated that the rate of sugar release was reduced in the transgenic plants. In addition, OXPdWND3A transgenic plants produced lower amounts of biomass than wild-type plants.Conclusions: PdWND3A affects lignin biosynthesis and composition and negatively impacts sugar releasemore »
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1618609
- Alternate Identifier(s):
- OSTI ID: 1626852; OSTI ID: 1761683
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Published Article
- Journal Name:
- BMC Plant Biology
- Additional Journal Information:
- Journal Name: BMC Plant Biology Journal Volume: 19 Journal Issue: 1; Journal ID: ISSN 1471-2229
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Plant Sciences; F5H; Lignin; Poplus; Saccharification; Sinapyl alcohol; S/G ratio; VND
Citation Formats
Yang, Yongil, Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., and Chen, Jin-Gui. PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus. United Kingdom: N. p., 2019.
Web. doi:10.1186/s12870-019-2111-5.
Yang, Yongil, Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., & Chen, Jin-Gui. PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus. United Kingdom. https://doi.org/10.1186/s12870-019-2111-5
Yang, Yongil, Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., and Chen, Jin-Gui. Mon .
"PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus". United Kingdom. https://doi.org/10.1186/s12870-019-2111-5.
@article{osti_1618609,
title = {PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus},
author = {Yang, Yongil and Yoo, Chang Geun and Rottmann, William and Winkeler, Kimberly A. and Collins, Cassandra M. and Gunter, Lee E. and Jawdy, Sara S. and Yang, Xiaohan and Pu, Yunqiao and Ragauskas, Arthur J. and Tuskan, Gerald A. and Chen, Jin-Gui},
abstractNote = {Background: Plant secondary cell wall is a renewable feedstock for biofuels and biomaterials production. Arabidopsis VASCULAR-RELATED NAC DOMAIN (VND) has been demonstrated to be a key transcription factor regulating secondary cell wall biosynthesis. However, less is known about its role in the woody species.Results: Here we report the functional characterization of Populus deltoides WOOD-ASSOCIATED NAC DOMAIN protein 3 (PdWND3A), a sequence homolog of Arabidopsis VND4 and VND5 that are members of transcription factor networks regulating secondary cell wall biosynthesis. PdWND3A was expressed at higher level in the xylem than in other tissues. The stem tissues of transgenic P. deltoides overexpressing PdWND3A (OXPdWND3A) contained more vessel cells than that of wild-type plants. Furthermore, lignin content and lignin monomer syringyl and guaiacyl (S/G) ratio were higher in OXPdWND3A transgenic plants than in wild-type plants. Consistent with these observations, the expression of FERULATE 5-HYDROXYLASE1 (F5H1), encoding an enzyme involved in the biosynthesis of sinapyl alcohol (S unit monolignol), was elevated in OXPdWND3A transgenic plants. Saccharification analysis indicated that the rate of sugar release was reduced in the transgenic plants. In addition, OXPdWND3A transgenic plants produced lower amounts of biomass than wild-type plants.Conclusions: PdWND3A affects lignin biosynthesis and composition and negatively impacts sugar release and biomass production.},
doi = {10.1186/s12870-019-2111-5},
journal = {BMC Plant Biology},
number = 1,
volume = 19,
place = {United Kingdom},
year = {2019},
month = {11}
}
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https://doi.org/10.1186/s12870-019-2111-5
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