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Title: PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus

Abstract

Background: Plant secondary cell wall is a renewable feedstock for biofuels and biomaterials production. Arabidopsis VASCULAR-RELATED NAC DOMAIN (VND) has been demonstrated to be a key transcription factor regulating secondary cell wall biosynthesis. However, less is known about its role in the woody species.Results: Here we report the functional characterization of Populus deltoides WOOD-ASSOCIATED NAC DOMAIN protein 3 (PdWND3A), a sequence homolog of Arabidopsis VND4 and VND5 that are members of transcription factor networks regulating secondary cell wall biosynthesis. PdWND3A was expressed at higher level in the xylem than in other tissues. The stem tissues of transgenic P. deltoides overexpressing PdWND3A (OXPdWND3A) contained more vessel cells than that of wild-type plants. Furthermore, lignin content and lignin monomer syringyl and guaiacyl (S/G) ratio were higher in OXPdWND3A transgenic plants than in wild-type plants. Consistent with these observations, the expression of FERULATE 5-HYDROXYLASE1 (F5H1), encoding an enzyme involved in the biosynthesis of sinapyl alcohol (S unit monolignol), was elevated in OXPdWND3A transgenic plants. Saccharification analysis indicated that the rate of sugar release was reduced in the transgenic plants. In addition, OXPdWND3A transgenic plants produced lower amounts of biomass than wild-type plants.Conclusions: PdWND3A affects lignin biosynthesis and composition and negatively impacts sugar releasemore » and biomass production.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1618609
Alternate Identifier(s):
OSTI ID: 1626852; OSTI ID: 1761683
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Published Article
Journal Name:
BMC Plant Biology
Additional Journal Information:
Journal Name: BMC Plant Biology Journal Volume: 19 Journal Issue: 1; Journal ID: ISSN 1471-2229
Publisher:
Springer Science + Business Media
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Plant Sciences; F5H; Lignin; Poplus; Saccharification; Sinapyl alcohol; S/G ratio; VND

Citation Formats

Yang, Yongil, Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., and Chen, Jin-Gui. PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus. United Kingdom: N. p., 2019. Web. doi:10.1186/s12870-019-2111-5.
Yang, Yongil, Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., & Chen, Jin-Gui. PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus. United Kingdom. doi:https://doi.org/10.1186/s12870-019-2111-5
Yang, Yongil, Yoo, Chang Geun, Rottmann, William, Winkeler, Kimberly A., Collins, Cassandra M., Gunter, Lee E., Jawdy, Sara S., Yang, Xiaohan, Pu, Yunqiao, Ragauskas, Arthur J., Tuskan, Gerald A., and Chen, Jin-Gui. Mon . "PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus". United Kingdom. doi:https://doi.org/10.1186/s12870-019-2111-5.
@article{osti_1618609,
title = {PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus},
author = {Yang, Yongil and Yoo, Chang Geun and Rottmann, William and Winkeler, Kimberly A. and Collins, Cassandra M. and Gunter, Lee E. and Jawdy, Sara S. and Yang, Xiaohan and Pu, Yunqiao and Ragauskas, Arthur J. and Tuskan, Gerald A. and Chen, Jin-Gui},
abstractNote = {Background: Plant secondary cell wall is a renewable feedstock for biofuels and biomaterials production. Arabidopsis VASCULAR-RELATED NAC DOMAIN (VND) has been demonstrated to be a key transcription factor regulating secondary cell wall biosynthesis. However, less is known about its role in the woody species.Results: Here we report the functional characterization of Populus deltoides WOOD-ASSOCIATED NAC DOMAIN protein 3 (PdWND3A), a sequence homolog of Arabidopsis VND4 and VND5 that are members of transcription factor networks regulating secondary cell wall biosynthesis. PdWND3A was expressed at higher level in the xylem than in other tissues. The stem tissues of transgenic P. deltoides overexpressing PdWND3A (OXPdWND3A) contained more vessel cells than that of wild-type plants. Furthermore, lignin content and lignin monomer syringyl and guaiacyl (S/G) ratio were higher in OXPdWND3A transgenic plants than in wild-type plants. Consistent with these observations, the expression of FERULATE 5-HYDROXYLASE1 (F5H1), encoding an enzyme involved in the biosynthesis of sinapyl alcohol (S unit monolignol), was elevated in OXPdWND3A transgenic plants. Saccharification analysis indicated that the rate of sugar release was reduced in the transgenic plants. In addition, OXPdWND3A transgenic plants produced lower amounts of biomass than wild-type plants.Conclusions: PdWND3A affects lignin biosynthesis and composition and negatively impacts sugar release and biomass production.},
doi = {10.1186/s12870-019-2111-5},
journal = {BMC Plant Biology},
number = 1,
volume = 19,
place = {United Kingdom},
year = {2019},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
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DOI: https://doi.org/10.1186/s12870-019-2111-5

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Works referenced in this record:

Lignin Biosynthesis
journal, June 2003


Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion
journal, January 2017

  • Yoo, Chang Geun; Yang, Yongil; Pu, Yunqiao
  • Green Chemistry, Vol. 19, Issue 22
  • DOI: 10.1039/C7GC02219K

Syringyl lignin production in conifers: Proof of concept in a Pine tracheary element system
journal, April 2015

  • Wagner, Armin; Tobimatsu, Yuki; Phillips, Lorelle
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 19
  • DOI: 10.1073/pnas.1411926112

Essential Role of Caffeoyl Coenzyme A O -Methyltransferase in Lignin Biosynthesis in Woody Poplar Plants
journal, October 2000

  • Zhong, Ruiqin; Morrison, W. Herbert; Himmelsbach, David S.
  • Plant Physiology, Vol. 124, Issue 2
  • DOI: 10.1104/pp.124.2.563

New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
journal, March 2010

  • Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
  • Systematic Biology, Vol. 59, Issue 3
  • DOI: 10.1093/sysbio/syq010

Metabolic Channeling in Plants
journal, June 2004


The poplar PtrWNDs are transcriptional activators of secondary cell wall biosynthesis
journal, April 2010


The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
journal, September 2007

  • Zhong, R.; Richardson, E. A.; Ye, Z.-H.
  • The Plant Cell Online, Vol. 19, Issue 9, p. 2776-2792
  • DOI: 10.1105/tpc.107.053678

Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms
journal, August 1999

  • Osakabe, K.; Tsao, C. C.; Li, L.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 16
  • DOI: 10.1073/pnas.96.16.8955

Arabidopsis NAC Domain Proteins, VND1 to VND5, Are Transcriptional Regulators of Secondary Wall Biosynthesis in Vessels
journal, August 2014


VASCULAR-RELATED NAC-DOMAIN 7 directly regulates the expression of a broad range of genes for xylem vessel formation: Direct target genes of VND7
journal, March 2011


NAC-MYB-based transcriptional regulation of secondary cell wall biosynthesis in land plants
journal, May 2015

  • Nakano, Yoshimi; Yamaguchi, Masatoshi; Endo, Hitoshi
  • Frontiers in Plant Science, Vol. 6
  • DOI: 10.3389/fpls.2015.00288

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Functional Characterization of Poplar Wood-Associated NAC Domain Transcription Factors
journal, December 2009

  • Zhong, Ruiqin; Lee, Chanhui; Ye, Zheng-Hua
  • Plant Physiology, Vol. 152, Issue 2
  • DOI: 10.1104/pp.109.148270

Phytozome: a comparative platform for green plant genomics
journal, November 2011

  • Goodstein, David M.; Shu, Shengqiang; Howson, Russell
  • Nucleic Acids Research, Vol. 40, Issue D1
  • DOI: 10.1093/nar/gkr944

The NAC Transcription Factors NST1 and NST2 of Arabidopsis Regulate Secondary Wall Thickenings and Are Required for Anther Dehiscence
journal, October 2005

  • Mitsuda, Nobutaka; Seki, Motoaki; Shinozaki, Kazuo
  • The Plant Cell, Vol. 17, Issue 11
  • DOI: 10.1105/tpc.105.036004

Secondary Cell Walls: Biosynthesis, Patterned Deposition and Transcriptional Regulation
journal, October 2014

  • Zhong, Ruiqin; Ye, Zheng-Hua
  • Plant and Cell Physiology, Vol. 56, Issue 2
  • DOI: 10.1093/pcp/pcu140

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
journal, December 2001


NIH Image to ImageJ: 25 years of image analysis
journal, June 2012

  • Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W.
  • Nature Methods, Vol. 9, Issue 7
  • DOI: 10.1038/nmeth.2089

SND1, a NAC Domain Transcription Factor, Is a Key Regulator of Secondary Wall Synthesis in Fibers of Arabidopsis
journal, November 2006


Cellulosic Biofuels
journal, June 2009


Transcription switches for protoxylem and metaxylem vessel formation
journal, August 2005


The Poplar MYB Master Switches Bind to the SMRE Site and Activate the Secondary Wall Biosynthetic Program during Wood Formation
journal, July 2013


Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014

  • Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
  • Science, Vol. 344, Issue 6185, p. 1246843-1246843
  • DOI: 10.1126/science.1246843

MYB Transcription Factors as Regulators of Phenylpropanoid Metabolism in Plants
journal, May 2015


Complexity of the transcriptional network controlling secondary wall biosynthesis
journal, December 2014


Phenylpropanoid Biosynthesis
journal, January 2010


Lignin content in natural Populus variants affects sugar release
journal, March 2011

  • Studer, M. H.; DeMartini, J. D.; Davis, M. F.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305
  • DOI: 10.1073/pnas.1009252108

The Pfam protein families database: towards a more sustainable future
journal, December 2015

  • Finn, Robert D.; Coggill, Penelope; Eberhardt, Ruth Y.
  • Nucleic Acids Research, Vol. 44, Issue D1
  • DOI: 10.1093/nar/gkv1344

Lignification: Flexibility, Biosynthesis and Regulation
journal, August 2016


Dissection of the Transcriptional Program Regulating Secondary Wall Biosynthesis during Wood Formation in Poplar
journal, September 2011

  • Zhong, Ruiqin; McCarthy, Ryan L.; Lee, Chanhui
  • Plant Physiology, Vol. 157, Issue 3
  • DOI: 10.1104/pp.111.181354

Molecular control of wood formation in trees
journal, March 2015

  • Ye, Zheng-Hua; Zhong, Ruiqin
  • Journal of Experimental Botany, Vol. 66, Issue 14
  • DOI: 10.1093/jxb/erv081

Multiple Classes of Transcription Factors Regulate the Expression of VASCULAR-RELATED NAC-DOMAIN7, a Master Switch of Xylem Vessel Differentiation
journal, September 2014

  • Endo, Hitoshi; Yamaguchi, Masatoshi; Tamura, Taizo
  • Plant and Cell Physiology, Vol. 56, Issue 2
  • DOI: 10.1093/pcp/pcu134

Basic local alignment search tool
journal, October 1990

  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

NAC Transcription Factors, NST1 and NST3, Are Key Regulators of the Formation of Secondary Walls in Woody Tissues of Arabidopsis
journal, January 2007

  • Mitsuda, N.; Iwase, A.; Yamamoto, H.
  • The Plant Cell, Vol. 19, Issue 1, p. 270-280
  • DOI: 10.1105/tpc.106.047043

Transcriptional networks for lignin biosynthesis: more complex than we thought?
journal, April 2011


New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase
journal, August 1999

  • Humphreys, J. M.; Hemm, M. R.; Chapple, C.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 18
  • DOI: 10.1073/pnas.96.18.10045

The Effects on Lignin Structure of Overexpression of Ferulate 5-Hydroxylase in Hybrid Poplar 1
journal, April 2009

  • Stewart, Jaclyn J.; Akiyama, Takuya; Chapple, Clint
  • Plant Physiology, Vol. 150, Issue 2
  • DOI: 10.1104/pp.109.137059

Global Analysis of Direct Targets of Secondary Wall NAC Master Switches in Arabidopsis
journal, November 2010

  • Zhong, Ruiqin; Lee, Chanhui; Ye, Zheng-Hua
  • Molecular Plant, Vol. 3, Issue 6
  • DOI: 10.1093/mp/ssq062

4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase
journal, April 2019


Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa
journal, January 2010


Plant cell wall polymers as precursors for biofuels
journal, June 2010


CINNAMYL ALCOHOL DEHYDROGENASE-C and -D Are the Primary Genes Involved in Lignin Biosynthesis in the Floral Stem of Arabidopsis
journal, June 2005

  • Sibout, Richard; Eudes, Aymerick; Mouille, Gregory
  • The Plant Cell, Vol. 17, Issue 7
  • DOI: 10.1105/tpc.105.030767

Syringyl lignin biosynthesis is directly regulated by a secondary cell wall master switch
journal, July 2010

  • Zhao, Q.; Wang, H.; Yin, Y.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 32, p. 14496-14501
  • DOI: 10.1073/pnas.1009170107

Modifications in Lignin and Accumulation of Phenolic Glucosides in Poplar Xylem upon Down-regulation of Caffeoyl-Coenzyme A O-Methyltransferase, an Enzyme Involved in Lignin Biosynthesis
journal, August 2000

  • Meyermans, Hugo; Morreel, Kris; Lapierre, Catherine
  • Journal of Biological Chemistry, Vol. 275, Issue 47, p. 36899-36909
  • DOI: 10.1074/jbc.M006915200

Altered lignin biosynthesis using biotechnology to improve lignocellulosic biofuel feedstocks
journal, July 2014

  • Poovaiah, Charleson R.; Nageswara-Rao, Madhugiri; Soneji, Jaya R.
  • Plant Biotechnology Journal, Vol. 12, Issue 9
  • DOI: 10.1111/pbi.12225

MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in Arabidopsis
journal, January 2009