Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O -acetyltransferase XOAT1

Lunin, Vladimir V.; Wang, Hsin-Tzu; Bharadwaj, Vivek S.; Alahuhta, Markus; Peña, Maria J.; Yang, Jeong-Yeh; Archer-Hartmann, Stephanie A.; Azadi, Parastoo; Himmel, Michael E.; Moremen, Kelley W.; York, William S.; Bomble, Yannick J.; Urbanowicz, Breeanna R.

doi:10.1105/tpc.20.00028

Title: Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O -acetyltransferase XOAT1

Full Record
Other Related Research

Authors:

^[1];

^[2];

^[1];

^[3];

^[1];

^[2];

^[1];

^[2]

Bioscience Center, National Renewable Energy Laboratory, Golden, Colorado 80401
Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602
Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602

Publication Date:: Thu Apr 30 00:00:00 EDT 2020

Sponsoring Org.:: USDOE

OSTI Identifier:: 1616767

Grant/Contract Number:: AC36-08GO28308; AC05-000R22725

Resource Type:: Published Article

Journal Name:: The Plant Cell

Additional Journal Information:: Journal Name: The Plant Cell Journal Volume: 32 Journal Issue: 7; Journal ID: ISSN 1040-4651

Publisher:: Oxford University Press

Country of Publication:: United States

Language:: English

Citation Formats


                    Lunin, Vladimir V., Wang, Hsin-Tzu, Bharadwaj, Vivek S., Alahuhta, Markus, Peña, Maria J., Yang, Jeong-Yeh, Archer-Hartmann, Stephanie A., Azadi, Parastoo, Himmel, Michael E., Moremen, Kelley W., York, William S., Bomble, Yannick J., and Urbanowicz, Breeanna R. Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O -acetyltransferase XOAT1.  United States: N. p., 2020. 
Web.  doi:10.1105/tpc.20.00028.

Copy to clipboard


                    Lunin, Vladimir V., Wang, Hsin-Tzu, Bharadwaj, Vivek S., Alahuhta, Markus, Peña, Maria J., Yang, Jeong-Yeh, Archer-Hartmann, Stephanie A., Azadi, Parastoo, Himmel, Michael E., Moremen, Kelley W., York, William S., Bomble, Yannick J., & Urbanowicz, Breeanna R. Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O -acetyltransferase XOAT1.  United States.  https://doi.org/10.1105/tpc.20.00028

Copy to clipboard


                    Lunin, Vladimir V., Wang, Hsin-Tzu, Bharadwaj, Vivek S., Alahuhta, Markus, Peña, Maria J., Yang, Jeong-Yeh, Archer-Hartmann, Stephanie A., Azadi, Parastoo, Himmel, Michael E., Moremen, Kelley W., York, William S., Bomble, Yannick J., and Urbanowicz, Breeanna R. Thu .  
"Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O -acetyltransferase XOAT1".  United States.  https://doi.org/10.1105/tpc.20.00028.

Copy to clipboard


                    
@article{osti_1616767,

  title        = {Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O -acetyltransferase XOAT1},

  author       = {Lunin, Vladimir V. and Wang, Hsin-Tzu and Bharadwaj, Vivek S. and Alahuhta, Markus and Peña, Maria J. and Yang, Jeong-Yeh and Archer-Hartmann, Stephanie A. and Azadi, Parastoo and Himmel, Michael E. and Moremen, Kelley W. and York, William S. and Bomble, Yannick J. and Urbanowicz, Breeanna R.},

  abstractNote = {},

  doi          = {10.1105/tpc.20.00028},

  journal      = {The Plant Cell},

  number       = 7,

  volume       = 32,

  place        = {United States},

  year         = {Thu Apr 30 00:00:00 EDT 2020},

  month        = {Thu Apr 30 00:00:00 EDT 2020}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Publisher's Version of Record
https://doi.org/10.1105/tpc.20.00028

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 24 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

Molecular Mechanism of Polysaccharide Acetylation by the Arabidopsis Xylan O-acetyltransferase XOAT1

Journal Article Lunin, Vladimir V. ; Wang, Hsin-Tzu ; Bharadwaj, Vivek S. ; ... - Plant Cell

Xylans are a major component of plant cell walls. O-Acetyl moieties are the dominant backbone substituents of glucuronoxylan in dicots and play a major role in the polymer-polymer interactions that are crucial for wall architecture and normal plant development. Here, we describe the biochemical, structural, and mechanistic characterization of Arabidopsis (Arabidopsis thaliana) xylan O-acetyltransferase 1 (XOAT1), a member of the plant-specific Trichome Birefringence Like (TBL) family. Detailed characterization of XOAT1-catalyzed reactions by real-time NMR confirms that it exclusively catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at bothmore »« less
https://doi.org/10.1105/tpc.20.00028
Overexpression of REDUCED WALL ACETYLATION C increases xylan acetylation and biomass recalcitrance in Populus

Journal Article Zhang, Jin ; Wang, Xiaqin ; Wang, Hsin-Tzu ; ... - Plant Physiology (Bethesda)

Plant lignocellulosic biomass, i.e. secondary cell walls of plants, is a vital alternative source for bioenergy. However, the acetylation of xylan in secondary cell walls impedes the conversion of biomass to biofuels. Previous studies have shown that REDUCED WALL ACETYLATION (RWA) proteins are directly involved in the acetylation of xylan but the regulatory mechanism of RWAs is not fully understood. In this study, we demonstrate that overexpression of a Populus trichocarpa PtRWA-C gene increases the level of xylan acetylation and increases the lignin content and S/G ratio, ultimately yielding poplar woody biomass with reduced saccharification efficiency. Furthermore, through gene coexpressionmore »« less
https://doi.org/10.1093/plphys/kiad377
Regiospecific Acetylation of Xylan is Mediated by a Group of DUF231-Containing O-Acetyltransferases

Journal Article Zhong, Ruiqin ; Cui, Dongtao ; Ye, Zheng-Hua - Plant and Cell Physiology

Not provided.
https://doi.org/10.1093/pcp/pcx147
Mutations of Arabidopsis TBL32 and TBL33 affect xylan acetylation and secondary wall deposition

Journal Article Yuan, Youxi ; Teng, Quincy ; Zhong, Ruiqin ; ... - PLoS ONE

Xylan is a major acetylated polymer in plant lignocellulosic biomass and it can be monoand di-acetylated at O-2 and O-3 as well as mono-acetylated at O-3 of xylosyl residues that is substituted with glucuronic acid (GlcA) at O-2. Based on the finding that ESK1, an Arabidopsis thaliana DUF231 protein, specifically mediates xylan 2-O- and 3-O-monoacetylation, we previously proposed that different acetyltransferase activities are required for regiospecific acetyl substitutions of xylan. Here, we demonstrate the functional roles of TBL32 and TBL33, two ESK1 close homologs, in acetyl substitutions of xylan. Simultaneous mutations of TBL32 and TBL33 resulted in a significant reductionmore »« less
Cited by 50
https://doi.org/10.1371/journal.pone.0146460

Full Text Available
Xyloglucan O-acetyltransferases from Arabidopsis thaliana and Populus trichocarpa catalyze acetylation of fucosylated galactose residues on xyloglucan side chains

Journal Article Zhong, Ruiqin ; Cui, Dongtao ; Ye, Zheng-Hua - Planta

Not provided.
https://doi.org/10.1007/s00425-018-2972-0

Similar Records