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Title: Ecogenomics reveals community interactions in a long-term methanogenic bioreactor and a rapid switch to sulfate-reducing conditions

Abstract

ABSTRACT The anaerobic digestion of wastes is globally important in the production of methane (CH4) as a biofuel. When sulfate is present, sulfate-reducing bacteria (SRB) are stimulated, competing with methanogens for common substrates, which decreases CH4 production and results in the formation of corrosive, odorous hydrogen sulfide gas (H2S). Here, we show that a population of SRB within a methanogenic bioreactor fed only butyrate for years immediately (within hours) responded to sulfate availability and shifted the microbial community dynamics within the bioreactor. By mapping shotgun metatranscriptomes to metagenome-assembled genomes, we shed light on the transcriptomic responses of key community members in response to increased sulfate provision. We link these short-term transcriptional responses to long-term niche partitioning using comparative metagenomic analyses. Our results suggest that sulfate provision supports a syntrophic butyrate oxidation community that disfavors poly-β-hydroxyalkanoate storage and that hydrogenotrophic SRB populations effectively exclude obligately hydrogenotrophic, but not aceticlastic, methanogens when sulfate is readily available. These findings elucidate key ecological dynamics between SRB, methanogens and syntrophic butyrate-oxidizing bacteria, which can be applied to a variety of engineered and natural systems.

Authors:
ORCiD logo [1];  [1]
  1. School of Civil and Environmental Engineering, Cornell University, 527 College Ave, Hollister Hall, Ithaca, NY, USA 14853
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1616635
Grant/Contract Number:  
503205; AC02–05CH11231
Resource Type:
Published Article
Journal Name:
FEMS Microbiology Ecology
Additional Journal Information:
Journal Name: FEMS Microbiology Ecology Journal Volume: 96 Journal Issue: 5; Journal ID: ISSN 0168-6496
Publisher:
Oxford University Press
Country of Publication:
Netherlands
Language:
English

Citation Formats

St. James, Andrew R., and Richardson, Ruth E. Ecogenomics reveals community interactions in a long-term methanogenic bioreactor and a rapid switch to sulfate-reducing conditions. Netherlands: N. p., 2020. Web. doi:10.1093/femsec/fiaa050.
St. James, Andrew R., & Richardson, Ruth E. Ecogenomics reveals community interactions in a long-term methanogenic bioreactor and a rapid switch to sulfate-reducing conditions. Netherlands. doi:https://doi.org/10.1093/femsec/fiaa050
St. James, Andrew R., and Richardson, Ruth E. Thu . "Ecogenomics reveals community interactions in a long-term methanogenic bioreactor and a rapid switch to sulfate-reducing conditions". Netherlands. doi:https://doi.org/10.1093/femsec/fiaa050.
@article{osti_1616635,
title = {Ecogenomics reveals community interactions in a long-term methanogenic bioreactor and a rapid switch to sulfate-reducing conditions},
author = {St. James, Andrew R. and Richardson, Ruth E.},
abstractNote = {ABSTRACT The anaerobic digestion of wastes is globally important in the production of methane (CH4) as a biofuel. When sulfate is present, sulfate-reducing bacteria (SRB) are stimulated, competing with methanogens for common substrates, which decreases CH4 production and results in the formation of corrosive, odorous hydrogen sulfide gas (H2S). Here, we show that a population of SRB within a methanogenic bioreactor fed only butyrate for years immediately (within hours) responded to sulfate availability and shifted the microbial community dynamics within the bioreactor. By mapping shotgun metatranscriptomes to metagenome-assembled genomes, we shed light on the transcriptomic responses of key community members in response to increased sulfate provision. We link these short-term transcriptional responses to long-term niche partitioning using comparative metagenomic analyses. Our results suggest that sulfate provision supports a syntrophic butyrate oxidation community that disfavors poly-β-hydroxyalkanoate storage and that hydrogenotrophic SRB populations effectively exclude obligately hydrogenotrophic, but not aceticlastic, methanogens when sulfate is readily available. These findings elucidate key ecological dynamics between SRB, methanogens and syntrophic butyrate-oxidizing bacteria, which can be applied to a variety of engineered and natural systems.},
doi = {10.1093/femsec/fiaa050},
journal = {FEMS Microbiology Ecology},
number = 5,
volume = 96,
place = {Netherlands},
year = {2020},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
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DOI: https://doi.org/10.1093/femsec/fiaa050

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