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Title: Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa

Abstract

Despite well established roles of microRNAs in plant development, few aspects have been addressed to understand their effects in seeds especially on lipid metabolism. In this study, we showed that overexpressing microRNA167A (miR167OE) in camelina (Camelina sativa) under a seed-specific promoter changed fatty acid composition and increased seed size. Specifically, the miR167OE seeds had a lower a-linolenic acid with a concomitantly higher linoleic acid content than the wild-type. This decreased level of fatty acid desatura- tion corresponded to a decreased transcriptional expression of the camelina fatty acid desaturase3 (CsFAD3) in developing seeds. MiR167 targeted the transcription factor auxin response factor (CsARF8) in camelina, as had been reported previously in Arabidopsis. Chromatin immunoprecipitation experiments combined with transcriptome analysis indicated that CsARF8 bound to promoters of camelina bZIP67 and ABI3 genes. These transcription factors directly or through the ABI3-bZIP12 pathway regulate CsFAD3 expression and affect a-linolenic acid accumulation. In addition, to decipher the miR167A-CsARF8 mediated transcriptional cascade for CsFAD3 suppression, transcriptome analysis was conducted to implicate mechanisms that regu- late seed size in camelina. Expression levels of many genes were altered in miR167OE, including orthologs that have previously been identified to affect seed size in other plants. Most notably, genes for seedmore » coat development such as suberin and lignin biosynthesis were down-regulated. This study provides valuable insights into the regulatory mechanism of fatty acid metabolism and seed size determination, and suggests possible approaches to improve these important traits in camelina.« less

Authors:
 [1];  [2];  [3];  [4]; ORCiD logo [1]
  1. Montana State Univ., Bozeman, MT (United States)
  2. Montana State Univ., Bozeman, MT (United States); Shanxi Agricultural Univ., Taigu (China)
  3. HudsonAlpha Inst. of Biotechnology, Huntsville, AL (United States)
  4. HudsonAlpha Inst. of Biotechnology, Huntsville, AL (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Montana State Univ., Bozeman, MT (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); USDA
OSTI Identifier:
1616082
Alternate Identifier(s):
OSTI ID: 1494844; OSTI ID: 1647217
Report Number(s):
1
Journal ID: ISSN 0960-7412; ark:/13030/qt27x8k3xz
Grant/Contract Number:  
AC02-05CH11231; SC00164900; DBI-1339358; SC0016400; DBI‐1339358; AC02‐05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
The Plant Journal
Additional Journal Information:
Journal Volume: 98; Journal Issue: 2; Journal ID: ISSN 0960-7412
Publisher:
Society for Experimental Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; microRNA 167; transcription factors; Camelina sativa; fatty acid desaturase 3; a-linolenic acid; seed size

Citation Formats

Na, GunNam, Mu, Xiaopeng, Grabowski, Paul, Schmutz, Jeremy, and Lu, Chaofu. Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa. United States: N. p., 2019. Web. doi:10.1111/tpj.14223.
Na, GunNam, Mu, Xiaopeng, Grabowski, Paul, Schmutz, Jeremy, & Lu, Chaofu. Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa. United States. doi:10.1111/tpj.14223.
Na, GunNam, Mu, Xiaopeng, Grabowski, Paul, Schmutz, Jeremy, and Lu, Chaofu. Thu . "Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa". United States. doi:10.1111/tpj.14223. https://www.osti.gov/servlets/purl/1616082.
@article{osti_1616082,
title = {Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa},
author = {Na, GunNam and Mu, Xiaopeng and Grabowski, Paul and Schmutz, Jeremy and Lu, Chaofu},
abstractNote = {Despite well established roles of microRNAs in plant development, few aspects have been addressed to understand their effects in seeds especially on lipid metabolism. In this study, we showed that overexpressing microRNA167A (miR167OE) in camelina (Camelina sativa) under a seed-specific promoter changed fatty acid composition and increased seed size. Specifically, the miR167OE seeds had a lower a-linolenic acid with a concomitantly higher linoleic acid content than the wild-type. This decreased level of fatty acid desatura- tion corresponded to a decreased transcriptional expression of the camelina fatty acid desaturase3 (CsFAD3) in developing seeds. MiR167 targeted the transcription factor auxin response factor (CsARF8) in camelina, as had been reported previously in Arabidopsis. Chromatin immunoprecipitation experiments combined with transcriptome analysis indicated that CsARF8 bound to promoters of camelina bZIP67 and ABI3 genes. These transcription factors directly or through the ABI3-bZIP12 pathway regulate CsFAD3 expression and affect a-linolenic acid accumulation. In addition, to decipher the miR167A-CsARF8 mediated transcriptional cascade for CsFAD3 suppression, transcriptome analysis was conducted to implicate mechanisms that regu- late seed size in camelina. Expression levels of many genes were altered in miR167OE, including orthologs that have previously been identified to affect seed size in other plants. Most notably, genes for seed coat development such as suberin and lignin biosynthesis were down-regulated. This study provides valuable insights into the regulatory mechanism of fatty acid metabolism and seed size determination, and suggests possible approaches to improve these important traits in camelina.},
doi = {10.1111/tpj.14223},
journal = {The Plant Journal},
number = 2,
volume = 98,
place = {United States},
year = {2019},
month = {1}
}

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    Works referencing / citing this record:

    Mapping quantitative trait loci for seed traits in Camelina sativa
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