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Title: Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin

Abstract

ERCC1 (excision repair cross complementing-group 1) is a mammalian endonuclease that incises the damaged strand of DNA during nucleotide excision repair and interstrand cross-link repair. Ercc1-/Δ mice, carrying one null and one hypomorphic Ercc1 allele, have been widely used to study aging due to accelerated aging phenotypes in numerous organs and their shortened lifespan. Ercc1-/Δ mice display combined features of human progeroid and cancer-prone syndromes. Although several studies report cellular senescence and apoptosis associated with the premature aging of Ercc1-/Δ mice, the link between these two processes and their physiological relevance in the phenotypes of Ercc1-/Δ mice are incompletely understood. Here, we show that ERCC1 depletion, both in cultured human fibroblasts and the skin of Ercc1-/Δ mice, initially induces cellular senescence and, importantly, increased expression of several SASP (senescence-associated secretory phenotype) factors. Cellular senescence induced by ERCC1 deficiency was dependent on activity of the p53 tumor-suppressor protein. In turn, TNFα secreted by senescent cells induced apoptosis, not only in neighboring ERCC1-deficient nonsenescent cells, but also cell autonomously in the senescent cells themselves. In addition, expression of the stem cell markers p63 and Lgr6 was significantly decreased in Ercc1-/Δ mouse skin, where the apoptotic cells are localized, compared to age-matched wild-typemore » skin, possibly due to the apoptosis of stem cells. These data suggest that ERCC1-depleted cells become susceptible to apoptosis via TNFα secreted from neighboring senescent cells. We speculate that parts of the premature aging phenotypes and shortened health- or lifespan may be due to stem cell depletion through apoptosis promoted by senescent cells.« less

Authors:
ORCiD logo [1];  [2];  [3];  [4];  [4];  [5];  [1];  [1]; ORCiD logo [6];  [1];  [7]
  1. Buck Institute for Research on Aging Novato CA USA
  2. Centre for Health Protection Research National Institute of Public Health and the Environment (RIVM) Bilthoven The Netherlands
  3. Department of Molecular Genetics Erasmus University Medical Center Rotterdam The Netherlands; Princess Máxima Center for Pediatric Oncology ONCODE Institute Utrecht The Netherlands
  4. CECAD Forschungszentrum Köln Germany
  5. Department of Molecular Genetics Erasmus University Medical Center Rotterdam The Netherlands; Princess Máxima Center for Pediatric Oncology ONCODE Institute Utrecht The Netherlands; CECAD Forschungszentrum Köln Germany
  6. Department of Molecular Medicine Sam and Ann Barshop Institute for Longevity and Aging Studies University of Texas Health Science Center San Antonio TX USA
  7. Buck Institute for Research on Aging Novato CA USA; Lawrence Berkeley National Laboratory Berkeley CA USA
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); European Research Council (ERC); KWO Dutch Cancer Society; German Research Foundation (DFG)
OSTI Identifier:
1615312
Grant/Contract Number:  
AC02-05CH11231; AG009909; AG017242; Dam2Age; 5030
Resource Type:
Accepted Manuscript
Journal Name:
Aging Cell
Additional Journal Information:
Journal Volume: 19; Journal Issue: 3; Journal ID: ISSN 1474-9718
Publisher:
Anatomical Society - Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; aging; cell death; DNA damage repair; senescence-associated secretory phenotype; tumor necrosis factor α

Citation Formats

Kim, Dong Eun, Dollé, Martijn E. T., Vermeij, Wilbert P., Gyenis, Akos, Vogel, Katharina, Hoeijmakers, Jan H. J., Wiley, Christopher D., Davalos, Albert R., Hasty, Paul, Desprez, Pierre‐Yves, and Campisi, Judith. Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin. United States: N. p., 2019. Web. https://doi.org/10.1111/acel.13072.
Kim, Dong Eun, Dollé, Martijn E. T., Vermeij, Wilbert P., Gyenis, Akos, Vogel, Katharina, Hoeijmakers, Jan H. J., Wiley, Christopher D., Davalos, Albert R., Hasty, Paul, Desprez, Pierre‐Yves, & Campisi, Judith. Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin. United States. https://doi.org/10.1111/acel.13072
Kim, Dong Eun, Dollé, Martijn E. T., Vermeij, Wilbert P., Gyenis, Akos, Vogel, Katharina, Hoeijmakers, Jan H. J., Wiley, Christopher D., Davalos, Albert R., Hasty, Paul, Desprez, Pierre‐Yves, and Campisi, Judith. Mon . "Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin". United States. https://doi.org/10.1111/acel.13072. https://www.osti.gov/servlets/purl/1615312.
@article{osti_1615312,
title = {Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin},
author = {Kim, Dong Eun and Dollé, Martijn E. T. and Vermeij, Wilbert P. and Gyenis, Akos and Vogel, Katharina and Hoeijmakers, Jan H. J. and Wiley, Christopher D. and Davalos, Albert R. and Hasty, Paul and Desprez, Pierre‐Yves and Campisi, Judith},
abstractNote = {ERCC1 (excision repair cross complementing-group 1) is a mammalian endonuclease that incises the damaged strand of DNA during nucleotide excision repair and interstrand cross-link repair. Ercc1-/Δ mice, carrying one null and one hypomorphic Ercc1 allele, have been widely used to study aging due to accelerated aging phenotypes in numerous organs and their shortened lifespan. Ercc1-/Δ mice display combined features of human progeroid and cancer-prone syndromes. Although several studies report cellular senescence and apoptosis associated with the premature aging of Ercc1-/Δ mice, the link between these two processes and their physiological relevance in the phenotypes of Ercc1-/Δ mice are incompletely understood. Here, we show that ERCC1 depletion, both in cultured human fibroblasts and the skin of Ercc1-/Δ mice, initially induces cellular senescence and, importantly, increased expression of several SASP (senescence-associated secretory phenotype) factors. Cellular senescence induced by ERCC1 deficiency was dependent on activity of the p53 tumor-suppressor protein. In turn, TNFα secreted by senescent cells induced apoptosis, not only in neighboring ERCC1-deficient nonsenescent cells, but also cell autonomously in the senescent cells themselves. In addition, expression of the stem cell markers p63 and Lgr6 was significantly decreased in Ercc1-/Δ mouse skin, where the apoptotic cells are localized, compared to age-matched wild-type skin, possibly due to the apoptosis of stem cells. These data suggest that ERCC1-depleted cells become susceptible to apoptosis via TNFα secreted from neighboring senescent cells. We speculate that parts of the premature aging phenotypes and shortened health- or lifespan may be due to stem cell depletion through apoptosis promoted by senescent cells.},
doi = {10.1111/acel.13072},
journal = {Aging Cell},
number = 3,
volume = 19,
place = {United States},
year = {2019},
month = {11}
}

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