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Title: Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae

Abstract

Bacteria and other types of microbes interact with their hosts in several ways, including metabolic pathways, development, and complex behavioral processes such as mate recognition. During the mating season, adult males of the lesser long-nosed agave pollinator bat Leptonycteris yerbabuenae (Phyllostomidae: Glossophaginae) develop a structure called the dorsal patch, which is located in the interscapular region and may play a role in kin recognition and mate selection. Using high-throughput sequencing of the V4 region of the 16S rRNA gene, we identified a total of 2,847 microbial phylotypes in the dorsal patches of eleven specimens. Twenty-six phylotypes were shared among all the patches, accounting for 30 to 75% of their relative abundance. These shared bacteria are distributed among 13 families, 10 orders, 6 classes and 3 phyla. Two of these common bacterial components of the dorsal patch are Lactococcus and Streptococcus. Some of them—Helcococcus, Aggregatibacter, Enterococcus, and Corynebacteriaceae—include bacteria with pathogenic potential. Half of the shared phylotypes belong to Gallicola, Anaerococcus, Peptoniphilus, Proteus, Staphylococcus, Clostridium, and Peptostreptococcus and specialize in fatty acid production through fermentative processes. This work lays the basis for future symbiotic microbe studies focused on communication and reproduction strategies in wildlife.

Authors:
ORCiD logo [1];  [2];  [3];  [4];  [5];  [3]
  1. Univ. Nacional Autonoma de Mexico (UNAM), Mexico City (Mexico). Inst. de EcologIa; Parque Cientifico y Tecnologico de Yucatan, Merida, Yucatan (Mexico). Lab. de Ecologia Bacteriana, Inst. de Ecologia, UNAM
  2. Consorcio de Investigación del Golfo de México (CIGOM), Mérida, Yucatán (México). Centro de Investigación y de Estudios Avanzados del Inst. Politécnico Nacional
  3. Parque Cientifico y Tecnologico de Yucatan, Merida, Yucatan (Mexico). Lab. de Ecologia Bacteriana, Inst. de Ecologia, UNAM
  4. Univ. Nacional Autonoma de Mexico (UNAM), Morelia, Michoacán, (Mexico). Lab. de Ecología Funcional, Inst. de Investigaciones en Ecosistemas y Sustentabilidad
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Climate and Ecosystem Sciences Division; Univ. of California, Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); Soluciones Ambientales Itzeni AC; SEP-CONACyT
OSTI Identifier:
1615280
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 14; Journal Issue: 12; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gaona, Osiris, Cerqueda-García, Daniel, Falcón, Luisa I., Vázquez-Domínguez, Guillermo, Valdespino-Castillo, Patricia M., and Neri-Barrios, Carla-Ximena. Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae. United States: N. p., 2019. Web. https://doi.org/10.1371/journal.pone.0226239.
Gaona, Osiris, Cerqueda-García, Daniel, Falcón, Luisa I., Vázquez-Domínguez, Guillermo, Valdespino-Castillo, Patricia M., & Neri-Barrios, Carla-Ximena. Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae. United States. https://doi.org/10.1371/journal.pone.0226239
Gaona, Osiris, Cerqueda-García, Daniel, Falcón, Luisa I., Vázquez-Domínguez, Guillermo, Valdespino-Castillo, Patricia M., and Neri-Barrios, Carla-Ximena. Mon . "Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae". United States. https://doi.org/10.1371/journal.pone.0226239. https://www.osti.gov/servlets/purl/1615280.
@article{osti_1615280,
title = {Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae},
author = {Gaona, Osiris and Cerqueda-García, Daniel and Falcón, Luisa I. and Vázquez-Domínguez, Guillermo and Valdespino-Castillo, Patricia M. and Neri-Barrios, Carla-Ximena},
abstractNote = {Bacteria and other types of microbes interact with their hosts in several ways, including metabolic pathways, development, and complex behavioral processes such as mate recognition. During the mating season, adult males of the lesser long-nosed agave pollinator bat Leptonycteris yerbabuenae (Phyllostomidae: Glossophaginae) develop a structure called the dorsal patch, which is located in the interscapular region and may play a role in kin recognition and mate selection. Using high-throughput sequencing of the V4 region of the 16S rRNA gene, we identified a total of 2,847 microbial phylotypes in the dorsal patches of eleven specimens. Twenty-six phylotypes were shared among all the patches, accounting for 30 to 75% of their relative abundance. These shared bacteria are distributed among 13 families, 10 orders, 6 classes and 3 phyla. Two of these common bacterial components of the dorsal patch are Lactococcus and Streptococcus. Some of them—Helcococcus, Aggregatibacter, Enterococcus, and Corynebacteriaceae—include bacteria with pathogenic potential. Half of the shared phylotypes belong to Gallicola, Anaerococcus, Peptoniphilus, Proteus, Staphylococcus, Clostridium, and Peptostreptococcus and specialize in fatty acid production through fermentative processes. This work lays the basis for future symbiotic microbe studies focused on communication and reproduction strategies in wildlife.},
doi = {10.1371/journal.pone.0226239},
journal = {PLoS ONE},
number = 12,
volume = 14,
place = {United States},
year = {2019},
month = {12}
}

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