RNA‐Seq analysis revealed the molecular mechanisms of photobiomodulation effect on human fibroblasts
[5]
- Institute for Electric Light Sources Fudan University Shanghai China, Engineering Research Centre of Advanced Lighting Technology Ministry of Education Shanghai China, Institute of Future Lighting Academy for Engineering and Technology Fudan University Shanghai China
- Institute for Electric Light Sources Fudan University Shanghai China, Engineering Research Centre of Advanced Lighting Technology Ministry of Education Shanghai China
- Department of Stomatology Huashan Hospital Fudan University Shanghai China
- Tongji University School of Medicine Stem Cell Translational Research Center Tongji Hospital Shanghai China
- Oral Biomedical Engineering Laboratory Shanghai Stomatological Hospital Fudan University Shanghai China
Abstract Background The photobiomodulation (PBM) effect has been applied to various clinical therapy for a long time. However, the mechanism related to the PBM effect in terms of wavelengths has been lack of in‐depth study, except that ultraviolet radiation has attracted much attention due to its strong cell‐killing effect. Purpose To clarify the principle behind PBM and the main mechanism of improvement. Methods To carry on this study, we created light equipment using three LED chips, which emit 390 nm ultraviolet radiation, 415 nm blue light and 660 nm red light, respectively. We choose human fibroblasts (HF) to be irradiated by three different wavelengths for PBM test. In this study, we used cell counting kit (CCK‐8) test to show the cell proliferation roughly and reported on a systematic RNA sequencing (RNA‐seq) analysis at transcriptional expression levels from HF, which accepted PBM of different wavelengths of light. Results We found that 415 nm blue light inhibited cell proliferation and 660 nm red light stimulated cell proliferation while 390 nm ultraviolet radiation has little influence on cell proliferation. Furthermore, RNA‐seq results showed that CSF1R, PPP3CC, ITGAL, ITGAM, IL2RB, and several other differentially expressed genes (DEGs) are involved in the cell proliferation. Relative DEGs values for matrix metalloproteinases (MMPs) gene family have shown a great difference in blue and red light radiation especially on MMP25, MMP9, MMP21, and MMP13. Conclusion Taken together, the results provide a valuable resource to describe the variation of HFs under PBM of different light at gene level.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1614802
- Journal Information:
- Photodermatology, Photoimmunology and Photomedicine, Journal Name: Photodermatology, Photoimmunology and Photomedicine Journal Issue: 4 Vol. 36; ISSN 0905-4383
- Publisher:
- Wiley-BlackwellCopyright Statement
- Country of Publication:
- Denmark
- Language:
- English
Similar Records
ACTINN: automated identification of cell types in single cell RNA sequencing
ACTINN: automated identification of cell types in single cell RNA sequencing