Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds

Anthony, Winston E.; Carr, Rhiannon R.; DeLorenzo, Drew M.; Campbell, Tayte P.; Shang, Zeyu; Foston, Marcus; Moon, Tae Seok; Dantas, Gautam

doi:10.1186/s13068-019-1535-3

Title: Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds

Journal Article · 05 August 2019 · Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-019-1535-3 · OSTI ID:1612925

^[1]; Carr, Rhiannon R. ^[1]; DeLorenzo, Drew M. ^[1]; Campbell, Tayte P. ^[1]; Shang, Zeyu ^[1]; Foston, Marcus ^[1]; Moon, Tae Seok ^[1]; Dantas, Gautam ^[1]

Washington Univ., St. Louis, MO (United States)

The current extraction and use of fossil fuels has been linked to extensive negative health and environmental outcomes. Lignocellulosic biomass-derived biofuels and bioproducts are being actively considered as renewable alternatives to the fuels, chemicals, and materials produced from fossil fuels. A major challenge limiting large-scale, economic deployment of second-generation biorefineries is the insufficient product yield, diversity, and value that current conversion technologies can extract from lignocellulose, in particular from the underutilized lignin fraction. Rhodococcus opacus PD630 is an oleaginous gram-positive bacterium with innate catabolic pathways and tolerance mechanisms for the inhibitory aromatic compounds found in depolymerized lignin, as well as native or engineered pathways for hexose and pentose sugars found in the carbohydrate fractions of biomass. As a result, R. opacus holds potential as a biological chassis for the conversion of lignocellulosic biomass into biodiesel precursors and other value-added products. This review begins by examining the important role that lignin utilization will play in the future of biorefineries and by providing a concise survey of the current lignin conversion technologies. The genetic machinery and capabilities of R. opacus that allow the bacterium to tolerate and metabolize aromatic compounds and depolymerized lignin are also discussed, along with a synopsis of the genetic toolbox and synthetic biology methods now available for engineering this organism. Finally, we summarize the different feedstocks that R. opacus has been demonstrated to consume, and the high-value products that it has been shown to produce. Engineered R. opacus will enable lignin valorization over the coming years, leading to cost-effective conversion of lignocellulose into fuels, chemicals, and materials.

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Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0018324

OSTI ID:: 1612925

Journal Information:: Biotechnology for Biofuels, Vol. 12, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 32 works

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Physiological and genetic differences amongst Rhodococcus species for using glycerol as a source for growth and triacylglycerol production Herrero, O. Marisa; Moncalián, Gabriel; Alvarez, Héctor M. Microbiology, Vol. 162, Issue 2 https://doi.org/10.1099/mic.0.000232	journal	February 2016
Acid-based hydrolysis processes for ethanol from lignocellulosic materials: A review Taherzadeh, Mohammad J.; Karimi, Keikhosro BioResources, Vol. 2, Issue 3 https://doi.org/10.15376/biores.2.3.472-499	journal	August 2007
Construction of a Pair of Practical Nocardia-Escherichia coli Shuttle Vectors Chiba, Kazuhiro; Hoshino, Yasutaka; Ishino, Keiko Japanese Journal of Infectious Diseases, Vol. 60, Issue 1 https://doi.org/10.7883/yoken.JJID.2007.45	journal	February 2007

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