Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds
Abstract
The current extraction and use of fossil fuels has been linked to extensive negative health and environmental outcomes. Lignocellulosic biomass-derived biofuels and bioproducts are being actively considered as renewable alternatives to the fuels, chemicals, and materials produced from fossil fuels. A major challenge limiting large-scale, economic deployment of second-generation biorefineries is the insufficient product yield, diversity, and value that current conversion technologies can extract from lignocellulose, in particular from the underutilized lignin fraction. Rhodococcus opacus PD630 is an oleaginous gram-positive bacterium with innate catabolic pathways and tolerance mechanisms for the inhibitory aromatic compounds found in depolymerized lignin, as well as native or engineered pathways for hexose and pentose sugars found in the carbohydrate fractions of biomass. As a result, R. opacus holds potential as a biological chassis for the conversion of lignocellulosic biomass into biodiesel precursors and other value-added products. This review begins by examining the important role that lignin utilization will play in the future of biorefineries and by providing a concise survey of the current lignin conversion technologies. The genetic machinery and capabilities of R. opacus that allow the bacterium to tolerate and metabolize aromatic compounds and depolymerized lignin are also discussed, along with a synopsis of themore »
- Publication Date:
- Research Org.:
- Washington Univ., St. Louis, MO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1612925
- Grant/Contract Number:
- SC0018324
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; biotechnology & applied microbiology; energy & fuels; Rhodococcus opacus PD630; non-modal organism; lignocellulose; lignin; biofuel; bioproduct; aromatic compound; genetic tool; thermochemical conversion; biological conversion
Citation Formats
Anthony, Winston E., Carr, Rhiannon R., DeLorenzo, Drew M., Campbell, Tayte P., Shang, Zeyu, Foston, Marcus, Moon, Tae Seok, and Dantas, Gautam. Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds. United States: N. p., 2019.
Web. doi:10.1186/s13068-019-1535-3.
Anthony, Winston E., Carr, Rhiannon R., DeLorenzo, Drew M., Campbell, Tayte P., Shang, Zeyu, Foston, Marcus, Moon, Tae Seok, & Dantas, Gautam. Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds. United States. https://doi.org/10.1186/s13068-019-1535-3
Anthony, Winston E., Carr, Rhiannon R., DeLorenzo, Drew M., Campbell, Tayte P., Shang, Zeyu, Foston, Marcus, Moon, Tae Seok, and Dantas, Gautam. Mon .
"Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds". United States. https://doi.org/10.1186/s13068-019-1535-3. https://www.osti.gov/servlets/purl/1612925.
@article{osti_1612925,
title = {Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds},
author = {Anthony, Winston E. and Carr, Rhiannon R. and DeLorenzo, Drew M. and Campbell, Tayte P. and Shang, Zeyu and Foston, Marcus and Moon, Tae Seok and Dantas, Gautam},
abstractNote = {The current extraction and use of fossil fuels has been linked to extensive negative health and environmental outcomes. Lignocellulosic biomass-derived biofuels and bioproducts are being actively considered as renewable alternatives to the fuels, chemicals, and materials produced from fossil fuels. A major challenge limiting large-scale, economic deployment of second-generation biorefineries is the insufficient product yield, diversity, and value that current conversion technologies can extract from lignocellulose, in particular from the underutilized lignin fraction. Rhodococcus opacus PD630 is an oleaginous gram-positive bacterium with innate catabolic pathways and tolerance mechanisms for the inhibitory aromatic compounds found in depolymerized lignin, as well as native or engineered pathways for hexose and pentose sugars found in the carbohydrate fractions of biomass. As a result, R. opacus holds potential as a biological chassis for the conversion of lignocellulosic biomass into biodiesel precursors and other value-added products. This review begins by examining the important role that lignin utilization will play in the future of biorefineries and by providing a concise survey of the current lignin conversion technologies. The genetic machinery and capabilities of R. opacus that allow the bacterium to tolerate and metabolize aromatic compounds and depolymerized lignin are also discussed, along with a synopsis of the genetic toolbox and synthetic biology methods now available for engineering this organism. Finally, we summarize the different feedstocks that R. opacus has been demonstrated to consume, and the high-value products that it has been shown to produce. Engineered R. opacus will enable lignin valorization over the coming years, leading to cost-effective conversion of lignocellulose into fuels, chemicals, and materials.},
doi = {10.1186/s13068-019-1535-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 12,
place = {United States},
year = {2019},
month = {8}
}
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Works referenced in this record:
Enhanced production of carotenoids and lipids by Rhodococcus opacus PD630: Enhanced production of carotenoids and lipids by Rhodococcus opacus PD630
journal, February 2018
- Suwaleerat, Tharatron; Thanapimmetha, Anusith; Srisaiyoot, Maythee
- Journal of Chemical Technology & Biotechnology, Vol. 93, Issue 8
A Review on The Bioconversion of Lignin to Microbial Lipid with Oleaginous Rhodococcus opacus
journal, January 2017
- Mahan, Kristina M.; Le, Rosemary K.; Yuan, Joshua
- Journal of Biotechnology & Biomaterials, Vol. 07, Issue 02
Tolerance and adaptive evolution of triacylglycerol-producing Rhodococcus opacus to lignocellulose-derived inhibitors
journal, January 2015
- Kurosawa, Kazuhiko; Laser, Josephine; Sinskey, Anthony J.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Catechol 1,2-dioxygenase from the Gram-positive Rhodococcus opacus 1CP: Quantitative structure/activity relationship and the crystal structures of native enzyme and catechols adducts
journal, June 2010
- Matera, Irene; Ferraroni, Marta; Kolomytseva, Marina
- Journal of Structural Biology, Vol. 170, Issue 3
Biocatalytic conversion of lignin to aromatic dicarboxylic acids in Rhodococcus jostii RHA1 by re-routing aromatic degradation pathways
journal, January 2015
- Mycroft, Zoe; Gomis, Maria; Mines, Paul
- Green Chemistry, Vol. 17, Issue 11
Bioconversion of lignocellulosic pretreatment effluent via oleaginous Rhodococcus opacus DSM 1069
journal, January 2015
- Wells, Tyrone; Wei, Zhen; Ragauskas, Arthur
- Biomass and Bioenergy, Vol. 72
Tolerance and adaptive evolution of triacylglycerol-producing Rhodococcus opacus to lignocellulose-derived inhibitors
text, January 2015
- Kurosawa, Kazuhiko; Laser, Josephine; Sinskey, Anthony J.
- Technische Universität Berlin
Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005
- Lynd, Lee R.; van Zyl, Willem H.; McBride, John E.
- Current Opinion in Biotechnology, Vol. 16, Issue 5, p. 577-583
Recombineering in Mycobacterium tuberculosis
journal, December 2006
- van Kessel, Julia C.; Hatfull, Graham F.
- Nature Methods, Vol. 4, Issue 2
Escherichia coli-mycobacteria shuttle vectors for operon and gene fusions to lacZ: the pJEM series.
journal, January 1994
- Timm, J.; Lim, E. M.; Gicquel, B.
- Journal of Bacteriology, Vol. 176, Issue 21
Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization: Lipid production from whey by Rhodococcus
journal, May 2015
- Herrero, O. Marisa; Alvarez, Héctor M.
- European Journal of Lipid Science and Technology, Vol. 118, Issue 2
Molecular analysis and characterization of a broad-host-range plasmid, pEP2.
journal, January 1994
- Zhang, Y.; Praszkier, J.; Hodgson, A.
- Journal of Bacteriology, Vol. 176, Issue 18
Current and Future United States Light-Duty Vehicle Pathways: Cradle-to-Grave Lifecycle Greenhouse Gas Emissions and Economic Assessment
journal, January 2018
- Elgowainy, Amgad; Han, Jeongwoo; Ward, Jacob
- Environmental Science & Technology, Vol. 52, Issue 4
The Path Forward for Biofuels and Biomaterials
journal, January 2006
- Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
- Science, Vol. 311, Issue 5760, p. 484-489
Biodegradation and Rhodococcus – masters of catabolic versatility
journal, June 2005
- Larkin, Michael J.; Kulakov, Leonid A.; Allen, Christopher CR
- Current Opinion in Biotechnology, Vol. 16, Issue 3
Establishment of a gene transfer system for Rhodococcus opacus PD630 based on electroporation and its application for recombinant biosynthesis of poly(3-hydroxyalkanoic acids)
journal, October 1999
- Kalscheuer, R.; Arenskötter, M.; Steinbüchel, A.
- Applied Microbiology and Biotechnology, Vol. 52, Issue 4
Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels
journal, January 2014
- Kurosawa, Kazuhiko
- Journal of Microbial & Biochemical Technology, Vol. 06, Issue 05
High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production
journal, June 2010
- Kurosawa, Kazuhiko; Boccazzi, Paolo; de Almeida, Naomi M.
- Journal of Biotechnology, Vol. 147, Issue 3-4
Saccharification of Cellulose by Recombinant Rhodococcus opacus PD630 Strains
journal, June 2013
- Hetzler, Stephan; Bröker, Daniel; Steinbüchel, Alexander
- Applied and Environmental Microbiology, Vol. 79, Issue 17
Engineering l-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production
journal, July 2015
- Kurosawa, Kazuhiko; Plassmeier, Jens; Kalinowski, Jörn
- Metabolic Engineering, Vol. 30
Bacterial conversion of depolymerized Kraft lignin
journal, March 2019
- Ravi, Krithika; Abdelaziz, Omar Y.; Nöbel, Matthias
- Biotechnology for Biofuels, Vol. 12, Issue 1
Multi-catalysis reactions: new prospects and challenges of biotechnology to valorize lignin
journal, July 2012
- Gasser, Christoph A.; Hommes, Gregor; Schäffer, Andreas
- Applied Microbiology and Biotechnology, Vol. 95, Issue 5
Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630
journal, January 2018
- DeLorenzo, Drew M.; Rottinghaus, Austin G.; Henson, William R.
- ACS Synthetic Biology, Vol. 7, Issue 2
Continuous succinic acid production by Actinobacillus succinogenes on xylose-enriched hydrolysate
journal, November 2015
- Bradfield, Michael F. A.; Mohagheghi, Ali; Salvachúa, Davinia
- Biotechnology for Biofuels, Vol. 8, Issue 1
Bridging the Chemical and Biological Catalysis Gap: Challenges and Outlooks for Producing Sustainable Chemicals
journal, April 2014
- Schwartz, Thomas J.; O’Neill, Brandon J.; Shanks, Brent H.
- ACS Catalysis, Vol. 4, Issue 6
Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630
journal, April 2018
- DeLorenzo, Drew M.; Moon, Tae Seok
- Scientific Reports, Vol. 8, Issue 1
Efficient Electrotransformation of Corynebacterium diphtheriae with a Mini-Replicon Derived from the Corynebacterium glutamicum Plasmid pGA1
journal, November 2002
- Tauch, Andreas; Kirchner, Oliver; Löffler, Britta
- Current Microbiology, Vol. 45, Issue 5
Engineering of a Xylose Metabolic Pathway in Rhodococcus Strains
journal, May 2012
- Xiong, Xiaochao; Wang, Xi; Chen, Shulin
- Applied and Environmental Microbiology, Vol. 78, Issue 16
Cloning and Sequencing of the Gene which Encodes the Highly Inducible Acetamidase of Mycobacterium Smegmatis
journal, March 1993
- Mahenthiralingam, E.; Draper, P.; Davis, E. O.
- Journal of General Microbiology, Vol. 139, Issue 3
Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630
journal, October 2013
- Chen, Y.; Ding, Y.; Yang, L.
- Nucleic Acids Research, Vol. 42, Issue 2
Production of Clean Pyrolytic Sugars for Fermentation
journal, April 2014
- Rover, Marjorie R.; Johnston, Patrick A.; Jin, Tao
- ChemSusChem, Vol. 7, Issue 6, p. 1662-1668
Rapid metabolic analysis of Rhodococcus opacus PD630 via parallel 13 C-metabolite fingerprinting
journal, September 2015
- Hollinshead, Whitney D.; Henson, William R.; Abernathy, Mary
- Biotechnology and Bioengineering, Vol. 113, Issue 1
Characterization of a Novel Tectivirus Phage Toil and Its Potential as an Agent for Biolipid Extraction
journal, January 2018
- Gill, Jason J.; Wang, Baixin; Sestak, Emily
- Scientific Reports, Vol. 8, Issue 1
Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains
journal, March 2019
- Li, Xiaolu; He, Yucai; Zhang, Libing
- Biotechnology for Biofuels, Vol. 12, Issue 1
Catalytic Hydrogenolysis of Aryl Ethers: A Key Step in Lignin Valorization to Valuable Chemicals
journal, February 2015
- Zaheer, Muhammad; Kempe, Rhett
- ACS Catalysis, Vol. 5, Issue 3
Opportunities and challenges in biological lignin valorization
journal, December 2016
- Beckham, Gregg T.; Johnson, Christopher W.; Karp, Eric M.
- Current Opinion in Biotechnology, Vol. 42
Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus
journal, November 2014
- Wei, Zhen; Zeng, Guangming; Kosa, Matyas
- Applied Biochemistry and Biotechnology, Vol. 175, Issue 2
Review of US and EU initiatives toward development, demonstration, and commercialization of lignocellulosic biofuels
journal, November 2013
- Balan, Venkatesh; Chiaramonti, David; Kumar, Sandeep
- Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 6
Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630
journal, June 2017
- DeLorenzo, Drew M.; Henson, William R.; Moon, Tae Seok
- ACS Synthetic Biology, Vol. 6, Issue 10
Is it really the end of internal combustion engines and petroleum in transport?
journal, September 2018
- Kalghatgi, Gautam
- Applied Energy, Vol. 225
The DNA sequence and minimal replicon of the Corynebacterium glutamicum plasmid pSR1: evidence of a common ancestry with plasmids from C. diphtheriae
journal, August 1993
- Archer, J. A. C.; Sinskey, A. J.
- Journal of General Microbiology, Vol. 139, Issue 8
Review of US and EU initiatives toward development, demonstration, and commercialization of lignocellulosic biofuels
journal, August 2013
- Balan, Venkatesh; Chiaramonti, David; Kumar, Sandeep
- Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 6
Cultivation of lipid-producing bacteria with lignocellulosic biomass: Effects of inhibitory compounds of lignocellulosic hydrolysates
journal, June 2014
- Wang, Baixin; Rezenom, Yohannes H.; Cho, Kun-Ching
- Bioresource Technology, Vol. 161
Understanding Excess Emissions from Industrial Facilities: Evidence from Texas
journal, January 2018
- Zirogiannis, Nikolaos; Hollingsworth, Alex J.; Konisky, David M.
- Environmental Science & Technology, Vol. 52, Issue 5
Cellulosic Biofuels--Got Gasoline?
journal, August 2009
- Regalbuto, J. R.
- Science, Vol. 325, Issue 5942
Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates
journal, July 2015
- Wei, Zhen; Zeng, Guangming; Huang, Fang
- Applied Microbiology and Biotechnology, Vol. 99, Issue 17
Formation of intracytoplasmic lipid inclusions by Rhodococcus opacus strain PD630
journal, June 1996
- Alvarez, Hector M.; Mayer, Frank; Fabritius, Dirk
- Archives of Microbiology, Vol. 165, Issue 6
Biomass pyrolysis: a state-of-the-art review
journal, September 2008
- Babu, B. V.
- Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 5
Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production
journal, January 2013
- Kurosawa, Kazuhiko; Wewetzer, Sandra J.; Sinskey, Anthony J.
- Biotechnology for Biofuels, Vol. 6, Issue 1
Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms
journal, April 2018
- Yang, Shihui; Franden, Mary Ann; Yang, Qing
- Frontiers in Bioengineering and Biotechnology, Vol. 6
Detection of differentially culturable tubercle bacteria in sputum using mycobacterial culture filtrates
journal, March 2021
- Gordhan, Bhavna G.; Peters, Julian S.; McIvor, Amanda
- Scientific Reports, Vol. 11, Issue 1
Plasmid pGA1 from Corynebacterium glutamicum codes for a gene product that positively influences plasmid copy number.
journal, January 1997
- Nesvera, J.; Pátek, M.; Hochmannová, J.
- Journal of bacteriology, Vol. 179, Issue 5
Designer lignins: harnessing the plasticity of lignification
journal, February 2016
- Mottiar, Yaseen; Vanholme, Ruben; Boerjan, Wout
- Current Opinion in Biotechnology, Vol. 37
Initiation and Termination of DNA Transfer during Conjugation of IncI1 Plasmid R64: Roles of Two Sets of Inverted Repeat Sequences within oriT in Termination of R64 Transfer
journal, June 2000
- Furuya, Nobuhisa; Komano, Teruya
- Journal of Bacteriology, Vol. 182, Issue 11
A novel system for expressing recombinant proteins over a wide temperature range from 4 to 35°C: A Novel Recombinant Protein Expression System
journal, February 2004
- Nakashima, Nobutaka; Tamura, Tomohiro
- Biotechnology and Bioengineering, Vol. 86, Issue 2
Lignin Biosynthesis
journal, June 2003
- Boerjan, Wout; Ralph, John; Baucher, Marie
- Annual Review of Plant Biology, Vol. 54, Issue 1, p. 519-546
Catalysis Center for Energy Innovation for Biomass Processing: Research Strategies and Goals
journal, October 2010
- Vlachos, Dionisios G.; Chen, Jingguang G.; Gorte, Raymond J.
- Catalysis Letters, Vol. 140, Issue 3-4
IIIegitimate integration of non-replicative vectors in the genome of Rhodococcus fascians upon electro-transformation as an insertional mutagenesis system
journal, September 1991
- Desomer, J.; Crespi, M.; Van Montagu, M.
- Molecular Microbiology, Vol. 5, Issue 9
A review on the pretreatment of lignocellulose for high-value chemicals
journal, June 2017
- Chen, Hongyan; Liu, Jinbao; Chang, Xing
- Fuel Processing Technology, Vol. 160
Improving fatty acids production by engineering dynamic pathway regulation and metabolic control
journal, July 2014
- Xu, P.; Li, L.; Zhang, F.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 31
Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci
journal, January 2017
- Le, Rosemary K.; Wells Jr., Tyrone; Das, Parthapratim
- RSC Advances, Vol. 7, Issue 7
Kinetics of high strength phenol degradation using Bacillus brevis
journal, February 2006
- Arutchelvan, V.; Kanakasabai, V.; Elangovan, R.
- Journal of Hazardous Materials, Vol. 129, Issue 1-3
Construction of a BioBrick™ compatible vector system for Rhodococcus
journal, March 2017
- Ellinger, James; Schmidt-Dannert, Claudia
- Plasmid, Vol. 90
Enhancement of protocatechuate decarboxylase activity for the effective production of muconate from lignin-related aromatic compounds
journal, December 2014
- Sonoki, Tomonori; Morooka, Miyuki; Sakamoto, Kimitoshi
- Journal of Biotechnology, Vol. 192
Direct Combinatorial Pathway Optimization
journal, October 2016
- Coussement, Pieter; Bauwens, David; Maertens, Jo
- ACS Synthetic Biology, Vol. 6, Issue 2
Comparing the economic, energy, and environmental impacts of biodiesel versus petroleum diesel fuel use in construction equipment
journal, June 2018
- Lewis, Phil; Karimi, Boshra; Shan, Yongwei
- International Journal of Construction Education and Research, Vol. 15, Issue 4
Establishment of Cellobiose Utilization for Lipid Production in Rhodococcus opacus PD630
journal, February 2013
- Hetzler, Stephan; Steinbüchel, Alexander
- Applied and Environmental Microbiology, Vol. 79, Issue 9
Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis
journal, November 1990
- Snapper, S. B.; Melton, R. E.; Mustafa, S.
- Molecular Microbiology, Vol. 4, Issue 11
The atf2 gene is involved in triacylglycerol biosynthesis and accumulation in the oleaginous Rhodococcus opacus PD630
journal, August 2012
- Hernández, Martín A.; Arabolaza, Ana; Rodríguez, Eduardo
- Applied Microbiology and Biotechnology, Vol. 97, Issue 5
NpdR, a Repressor Involved in 2,4,6-Trinitrophenol Degradation in Rhodococcus opacus HL PM-1
journal, January 2004
- Nga, Dang P.; Altenbuchner, Josef; Heiss, Gesche S.
- Journal of Bacteriology, Vol. 186, Issue 1
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007
- Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
- Science, Vol. 315, Issue 5813, p. 804-807
Host-vector system for phenol-degrading Rhodococcus erythropolis based on Corynebacterium plasmids
journal, February 2003
- Veselý, M.; Pátek, M.; Nešvera, J.
- Applied Microbiology and Biotechnology, Vol. 61, Issue 5-6
Biodegradation potential of the genus Rhodococcus
journal, January 2009
- Martinkova, L.; Uhnakova, B.; Patek, M.
- Environment International, Vol. 35, Issue 1
Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a "mini" mycobacterium-Escherichia coli shuttle vector.
journal, January 1990
- Ranes, M. G.; Rauzier, J.; Lagranderie, M.
- Journal of Bacteriology, Vol. 172, Issue 5
Biodiesel production potential of oleaginous Rhodococcus opacus grown on biomass gasification wastewater
journal, May 2017
- Goswami, Lalit; Tejas Namboodiri, M. M.; Vinoth Kumar, R.
- Renewable Energy, Vol. 105
Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform
journal, February 2017
- Rock, Jeremy M.; Hopkins, Forrest F.; Chavez, Alejandro
- Nature Microbiology, Vol. 2, Issue 4
Stochastic model for electric field-induced membrane pores electroporation
journal, May 1984
- Sugar, I. P.; Neumann, E.
- Biophysical Chemistry, Vol. 19, Issue 3
Developments in crops and management systems to improve lignocellulosic feedstock production
journal, July 2013
- Coulman, Bruce; Dalai, Ajay; Heaton, Emily
- Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 5
Synthetic promoter design for new microbial chassis
journal, June 2016
- Gilman, James; Love, John
- Biochemical Society Transactions, Vol. 44, Issue 3
Green and sustainable manufacture of chemicals from biomass: state of the art
journal, January 2014
- Sheldon, Roger A.
- Green Chem., Vol. 16, Issue 3
Lignin valorization through integrated biological funneling and chemical catalysis
journal, August 2014
- Linger, J. G.; Vardon, D. R.; Guarnieri, M. T.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018
Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin
journal, July 2018
- Becker, Judith; Kuhl, Martin; Kohlstedt, Michael
- Microbial Cell Factories, Vol. 17, Issue 1
Beta-ketoadipic acid and muconolactone production from a lignin-related aromatic compound through the protocatechuate 3,4-metabolic pathway
journal, June 2016
- Okamura-Abe, Yuriko; Abe, Tomokuni; Nishimura, Kei
- Journal of Bioscience and Bioengineering, Vol. 121, Issue 6
Compositional characterization and imaging of “wall-bound” acylesters of Populus trichocarpa reveal differential accumulation of acyl molecules in normal and reactive woods
journal, September 2008
- Gou, Jin-Ying; Park, Simone; Yu, Xiao-Hong
- Planta, Vol. 229, Issue 1
Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.
journal, February 1996
- Pelicic, V.; Reyrat, J. M.; Gicquel, B.
- Journal of Bacteriology, Vol. 178, Issue 4
Lignin Biosynthesis and Structure
journal, May 2010
- Vanholme, R.; Demedts, B.; Morreel, K.
- Plant Physiology, Vol. 153, Issue 3, p. 895-905
Bioprocess development for muconic acid production from aromatic compounds and lignin
journal, January 2018
- Salvachúa, Davinia; Johnson, Christopher W.; Singer, Christine A.
- Green Chemistry, Vol. 20, Issue 21
CRISPR interference (CRISPRi) for sequence-specific control of gene expression
journal, October 2013
- Larson, Matthew H.; Gilbert, Luke A.; Wang, Xiaowo
- Nature Protocols, Vol. 8, Issue 11
Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014
- Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
- Science, Vol. 344, Issue 6185, p. 1246843-1246843
Boosting fatty acid synthesis in Rhodococcus opacus PD630 by overexpression of autologous thioesterases
journal, March 2016
- Huang, Luxuan; Zhao, Lina; Zan, Xinyi
- Biotechnology Letters, Vol. 38, Issue 6
Comparative transcriptomics elucidates adaptive phenol tolerance and utilization in lipid-accumulating Rhodococcus opacus PD630
journal, February 2016
- Yoneda, Aki; Henson, William R.; Goldner, Nicholas K.
- Nucleic Acids Research, Vol. 44, Issue 5
Pathways for degradation of lignin in bacteria and fungi
journal, January 2011
- Bugg, Timothy D. H.; Ahmad, Mark; Hardiman, Elizabeth M.
- Natural Product Reports, Vol. 28, Issue 12, p. 1883-1896
Directed bioconversion of Kraft lignin to polyhydroxyalkanoate by Cupriavidus basilensis B-8 without any pretreatment
journal, January 2017
- Shi, Yan; Yan, Xu; Li, Qiang
- Process Biochemistry, Vol. 52
Multi-omic elucidation of aromatic catabolism in adaptively evolved Rhodococcus opacus
journal, September 2018
- Henson, William R.; Campbell, Tayte; DeLorenzo, Drew M.
- Metabolic Engineering, Vol. 49
Pyrolysis of Wood/Biomass for Bio-oil: A Critical Review
journal, May 2006
- Mohan, Dinesh; Pittman,, Charles U.; Steele, Philip H.
- Energy & Fuels, Vol. 20, Issue 3, p. 848-889
Lipid Production from Dilute Alkali Corn Stover Lignin by Rhodococcus Strains
journal, January 2017
- He, Yucai; Li, Xiaolu; Ben, Haoxi
- ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 3
Improved glycerol utilization by a triacylglycerol-producing Rhodococcus opacus strain for renewable fuels
journal, January 2015
- Kurosawa, Kazuhiko; Radek, Andreas; Plassmeier, Jens K.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Cloning and characterization of a gene involved in triacylglycerol biosynthesis and identification of additional homologous genes in the oleaginous bacterium Rhodococcus opacus PD630
journal, August 2008
- Alvarez, A. F.; Alvarez, H. M.; Kalscheuer, R.
- Microbiology, Vol. 154, Issue 8
Biodegradation kinetics of phenol and catechol using Pseudomonas putida MTCC 1194
journal, January 2005
- Kumar, Arinjay; Kumar, Shashi; Kumar, Surendra
- Biochemical Engineering Journal, Vol. 22, Issue 2
Empirical equations and economical study for blending biofuel with petroleum jet fuel
journal, January 2018
- ElGalad, M. I.; El- Khatib, K. M.; Abdelkader, E.
- Journal of Advanced Research, Vol. 9
The Catalytic Valorization of Lignin for the Production of Renewable Chemicals
journal, June 2010
- Zakzeski, Joseph; Bruijnincx, Pieter C. A.; Jongerius, Anna L.
- Chemical Reviews, Vol. 110, Issue 6, p. 3552-3599
Genome-based analysis for the identification of genes involved in o-xylene degradation in Rhodococcus opacus R7
journal, August 2018
- Di Canito, Alessandra; Zampolli, Jessica; Orro, Alessandro
- BMC Genomics, Vol. 19, Issue 1
Rhodococcus bacteria as a promising source of oils from olive mill wastes
journal, July 2018
- Herrero, O. Marisa; Villalba, María S.; Lanfranconi, Mariana P.
- World Journal of Microbiology and Biotechnology, Vol. 34, Issue 8
Breaking Down Lignin to High-Value Chemicals: The Conversion of Lignocellulose to Vanillin in a Gene Deletion Mutant of Rhodococcus jostii RHA1
journal, August 2013
- Sainsbury, Paul D.; Hardiman, Elizabeth M.; Ahmad, Mark
- ACS Chemical Biology, Vol. 8, Issue 10
Bioconversion of oxygen-pretreated Kraft lignin to microbial lipid with oleaginous Rhodococcus opacus DSM 1069
journal, January 2015
- Wei, Zhen; Zeng, Guangming; Huang, Fang
- Green Chemistry, Vol. 17, Issue 5
Rewiring neutral lipids production for the de novo synthesis of wax esters in Rhodococcus opacus PD630
journal, October 2017
- Lanfranconi, Mariana P.; Alvarez, Héctor M.
- Journal of Biotechnology, Vol. 260
Synergistic enzymatic and microbial lignin conversion
journal, January 2016
- Zhao, Cheng; Xie, Shangxian; Pu, Yunqiao
- Green Chemistry, Vol. 18, Issue 5
A Novel p-Nitrophenol Degradation Gene Cluster from a Gram-Positive Bacterium, Rhodococcus opacus SAO101
journal, August 2004
- Kitagawa, Wataru; Kimura, Nobutada; Kamagata, Yoichi
- Journal of Bacteriology, Vol. 186, Issue 15
Advanced Chemical Design for Efficient Lignin Bioconversion
journal, January 2017
- Xie, Shangxian; Sun, Qining; Pu, Yunqiao
- ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 3
Peak oil, 20 years later: Failed prediction or useful insight?
journal, February 2019
- Bardi, Ugo
- Energy Research & Social Science, Vol. 48
Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept
journal, May 2009
- Kaparaju, Prasad; Serrano, María; Thomsen, Anne Belinda
- Bioresource Technology, Vol. 100, Issue 9
Isolation and characterization of the Rhodococcus opacus thiostrepton-inducible genes tipAL and tipAS : application for recombinant protein expression in Rhodococcus
journal, August 2004
- Dong, Li; Nakashima, Nobutaka; Tamura, Noriko
- FEMS Microbiology Letters, Vol. 237, Issue 1
Biosynthesis of cis, cis-Muconic Acid and Its Aromatic Precursors, Catechol and Protocatechuic Acid, from Renewable Feedstocks by Saccharomyces cerevisiae
journal, September 2012
- Weber, Christian; Brückner, Christine; Weinreb, Sheila
- Applied and Environmental Microbiology, Vol. 78, Issue 23, p. 8421-8430
Comparative and Functional Genomics of Rhodococcus opacus PD630 for Biofuels Development
journal, September 2011
- Holder, Jason W.; Ulrich, Jil C.; DeBono, Anthony C.
- PLoS Genetics, Vol. 7, Issue 9
The new forestry biofuels sector
journal, January 2008
- Pu, Yunqiao; Zhang, Dongcheng; Singh, Preet M.
- Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 1
Physiological and genetic differences amongst Rhodococcus species for using glycerol as a source for growth and triacylglycerol production
journal, February 2016
- Herrero, O. Marisa; Moncalián, Gabriel; Alvarez, Héctor M.
- Microbiology, Vol. 162, Issue 2
Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressor
journal, January 2005
- Ehrt, S.
- Nucleic Acids Research, Vol. 33, Issue 2
Effect of Aromatic Compounds on Cellular Fatty Acid Composition of Rhodococcus opacus
journal, January 1999
- Tsitko, Irina V.; Zaitsev, Gennadi M.; Lobanok, Anatoli G.
- Applied and Environmental Microbiology, Vol. 65, Issue 2
Utilization of Halogenated Benzenes, Phenols, and Benzoates by Rhodococcus opacus GM-14.
journal, January 1995
- Zaitsev, G. M.; Uotila, J. S.; Tsitko, I. V.
- Applied and environmental microbiology, Vol. 61, Issue 12
Biochemistry of homologous recombination in Escherichia coli.
journal, January 1994
- Kowalczykowski, S. C.; Dixon, D. A.; Eggleston, A. K.
- Microbiological Reviews, Vol. 58, Issue 3
Understanding Excess Emissions from Industrial Facilities: Evidence from Texas
journal, January 2019
- Zirogiannis, Nikolaos; Hollingsworth, Alex; Konisky, David
- SSRN Electronic Journal