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Title: Identification of Key Enzymes for Pectin Synthesis in Seed Mucilage

Abstract

Pectin is a vital component of the plant cell wall, and provides the molecular glue that maintains cell-cell adhesion, among other functions. As the most complex wall polysaccharide, pectin is composed of several covalently-linked domains, such as homogalacturonan (HG) and rhamnogalacturonan I (RG I). Pectin has widespread uses in the food industry and has emerging biomedical applications, but its synthesis remains poorly understood. For instance, the enzymes that catalyze RG I elongation remain unknown. Recently, a co-expression and sequence-based MUCILAGE-RELATED (MUCI) reverse genetic screen uncovered hemicellulose biosynthetic enzymes in the Arabidopsis thaliana seed coat. Here, we use an extension of this strategy to identify MUCI70 as the founding member of a glycosyltransferase family essential for the accumulation of seed mucilage, a gelatinous wall rich in unbranched RG I. Detailed biochemical and histological characterization of two muci70 mutants and two gaut11 mutants identified MUCI70 and GAUT11 as required for two distinct RG I domains in seed mucilage. We demonstrate that, unlike MUCI70, GAUT11 catalyzes HG elongation in vitro, and is thus likely required for the synthesis of an HG region important for RG I elongation. Analysis of a muci70 gaut11 double mutant confirmed that MUCI70 and GAUT11 are indispensable for themore » production and release of the bulk of mucilage RG I, and for shaping the surface morphology of seeds. In addition, we uncover relationships between pectin and hemicelluloses and show that xylan is essential for the elongation of at least one RG I domain.« less

Authors:
ORCiD logo [1]; ORCiD logo [2];  [3]; ORCiD logo [3];  [1]; ORCiD logo [2];  [2];  [2]; ORCiD logo [1]
  1. Forschungszentrum Jülich (Germany); Aachen Univ. (Germany)
  2. Univ. of Georgia, Athens, GA (United States)
  3. Forschungszentrum Jülich (Germany)
Publication Date:
Research Org.:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); Natural Sciences and Engineering Research Council of Canada (NERSC); German Research Foundation (DFG); Ministry of Innovation, Science and Research of North-Rhine Westphalia; National Institutes of Health (NIH); National Science Foundation (NSF)
OSTI Identifier:
1612316
Grant/Contract Number:  
SC0015662; PS02-06ER64304; P41GM103390; P01GM107012
Resource Type:
Accepted Manuscript
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Name: Plant Physiology (Bethesda); Journal Volume: 178; Journal Issue: 3; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; plant sciences

Citation Formats

Voiniciuc, Cătălin, Engle, Kristen A., Günl, Markus, Dieluweit, Sabine, Schmidt, Maximilian Heinrich-Wilhelm, Yang, Jeong-Yeh, Moremen, Kelley W., Mohnen, Debra, and Usadel, Björn. Identification of Key Enzymes for Pectin Synthesis in Seed Mucilage. United States: N. p., 2018. Web. doi:10.1104/pp.18.00584.
Voiniciuc, Cătălin, Engle, Kristen A., Günl, Markus, Dieluweit, Sabine, Schmidt, Maximilian Heinrich-Wilhelm, Yang, Jeong-Yeh, Moremen, Kelley W., Mohnen, Debra, & Usadel, Björn. Identification of Key Enzymes for Pectin Synthesis in Seed Mucilage. United States. doi:10.1104/pp.18.00584.
Voiniciuc, Cătălin, Engle, Kristen A., Günl, Markus, Dieluweit, Sabine, Schmidt, Maximilian Heinrich-Wilhelm, Yang, Jeong-Yeh, Moremen, Kelley W., Mohnen, Debra, and Usadel, Björn. Sat . "Identification of Key Enzymes for Pectin Synthesis in Seed Mucilage". United States. doi:10.1104/pp.18.00584. https://www.osti.gov/servlets/purl/1612316.
@article{osti_1612316,
title = {Identification of Key Enzymes for Pectin Synthesis in Seed Mucilage},
author = {Voiniciuc, Cătălin and Engle, Kristen A. and Günl, Markus and Dieluweit, Sabine and Schmidt, Maximilian Heinrich-Wilhelm and Yang, Jeong-Yeh and Moremen, Kelley W. and Mohnen, Debra and Usadel, Björn},
abstractNote = {Pectin is a vital component of the plant cell wall, and provides the molecular glue that maintains cell-cell adhesion, among other functions. As the most complex wall polysaccharide, pectin is composed of several covalently-linked domains, such as homogalacturonan (HG) and rhamnogalacturonan I (RG I). Pectin has widespread uses in the food industry and has emerging biomedical applications, but its synthesis remains poorly understood. For instance, the enzymes that catalyze RG I elongation remain unknown. Recently, a co-expression and sequence-based MUCILAGE-RELATED (MUCI) reverse genetic screen uncovered hemicellulose biosynthetic enzymes in the Arabidopsis thaliana seed coat. Here, we use an extension of this strategy to identify MUCI70 as the founding member of a glycosyltransferase family essential for the accumulation of seed mucilage, a gelatinous wall rich in unbranched RG I. Detailed biochemical and histological characterization of two muci70 mutants and two gaut11 mutants identified MUCI70 and GAUT11 as required for two distinct RG I domains in seed mucilage. We demonstrate that, unlike MUCI70, GAUT11 catalyzes HG elongation in vitro, and is thus likely required for the synthesis of an HG region important for RG I elongation. Analysis of a muci70 gaut11 double mutant confirmed that MUCI70 and GAUT11 are indispensable for the production and release of the bulk of mucilage RG I, and for shaping the surface morphology of seeds. In addition, we uncover relationships between pectin and hemicelluloses and show that xylan is essential for the elongation of at least one RG I domain.},
doi = {10.1104/pp.18.00584},
journal = {Plant Physiology (Bethesda)},
number = 3,
volume = 178,
place = {United States},
year = {2018},
month = {10}
}

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