Engineered microbial biofuel production and recovery under supercritical carbon dioxide
Abstract
Culture contamination, end-product toxicity, and energy efficient product recovery are long-standing bioprocess challenges. To solve these problems, we propose a high-pressure fermentation strategy, coupled with in situ extraction using the abundant and renewable solvent supercritical carbon dioxide (scCO2), which is also known for its broad microbial lethality. Towards this goal, we report the domestication and engineering of a scCO2-tolerant strain of Bacillus megaterium, previously isolated from formation waters from the McElmo Dome CO2 field, to produce branched alcohols that have potential use as biofuels. After establishing induced-expression under scCO2, isobutanol production from 2-ketoisovalerate is observed with greater than 40% yield with co-produced isopentanol. Finally, we present a process model to compare the energy required for our process to other in situ extraction methods, such as gas stripping, finding scCO2 extraction to be potentially competitive, if not superior.
- Authors:
-
[1];
[3];
[2];
[1];
[1]
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- Worcester Polytechnic Institute, MA (United States)
- Worcester Polytechnic Institute, MA (United States); Univ. of Bath (United Kingdom)
- Publication Date:
- Research Org.:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1611850
- Grant/Contract Number:
- SC0012555
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Science & Technology - Other Topics
Citation Formats
Boock, Jason T., Freedman, Adam J. E., Tompsett, Geoffrey A., Muse, Sarah K., Allen, Audrey J., Jackson, Luke A., Castro-Dominguez, Bernardo, Timko, Michael T., Prather, Kristala L. J., and Thompson, Janelle R. Engineered microbial biofuel production and recovery under supercritical carbon dioxide. United States: N. p., 2019.
Web. doi:10.1038/s41467-019-08486-6.
Boock, Jason T., Freedman, Adam J. E., Tompsett, Geoffrey A., Muse, Sarah K., Allen, Audrey J., Jackson, Luke A., Castro-Dominguez, Bernardo, Timko, Michael T., Prather, Kristala L. J., & Thompson, Janelle R. Engineered microbial biofuel production and recovery under supercritical carbon dioxide. United States. https://doi.org/10.1038/s41467-019-08486-6
Boock, Jason T., Freedman, Adam J. E., Tompsett, Geoffrey A., Muse, Sarah K., Allen, Audrey J., Jackson, Luke A., Castro-Dominguez, Bernardo, Timko, Michael T., Prather, Kristala L. J., and Thompson, Janelle R. Mon .
"Engineered microbial biofuel production and recovery under supercritical carbon dioxide". United States. https://doi.org/10.1038/s41467-019-08486-6. https://www.osti.gov/servlets/purl/1611850.
@article{osti_1611850,
title = {Engineered microbial biofuel production and recovery under supercritical carbon dioxide},
author = {Boock, Jason T. and Freedman, Adam J. E. and Tompsett, Geoffrey A. and Muse, Sarah K. and Allen, Audrey J. and Jackson, Luke A. and Castro-Dominguez, Bernardo and Timko, Michael T. and Prather, Kristala L. J. and Thompson, Janelle R.},
abstractNote = {Culture contamination, end-product toxicity, and energy efficient product recovery are long-standing bioprocess challenges. To solve these problems, we propose a high-pressure fermentation strategy, coupled with in situ extraction using the abundant and renewable solvent supercritical carbon dioxide (scCO2), which is also known for its broad microbial lethality. Towards this goal, we report the domestication and engineering of a scCO2-tolerant strain of Bacillus megaterium, previously isolated from formation waters from the McElmo Dome CO2 field, to produce branched alcohols that have potential use as biofuels. After establishing induced-expression under scCO2, isobutanol production from 2-ketoisovalerate is observed with greater than 40% yield with co-produced isopentanol. Finally, we present a process model to compare the energy required for our process to other in situ extraction methods, such as gas stripping, finding scCO2 extraction to be potentially competitive, if not superior.},
doi = {10.1038/s41467-019-08486-6},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {Mon Feb 04 00:00:00 EST 2019},
month = {Mon Feb 04 00:00:00 EST 2019}
}
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