NMR Methods to Characterize Protein-Ligand Interactions

Maity, Sanhita; Gundampati, Ravi Kumar; Suresh Kumar, Thallapuranam Krishnaswamy

doi:10.1177/1934578x19849296

Title: NMR Methods to Characterize Protein-Ligand Interactions

Journal Article · 27 May 2019 · Natural Product Communications

DOI: https://doi.org/10.1177/1934578x19849296 · OSTI ID:1609352

Maity, Sanhita ^[1]; Gundampati, Ravi Kumar ^[1]; Suresh Kumar, Thallapuranam Krishnaswamy ^[1]

Univ. of Arkansas, Fayetteville, AR (United States)

Structural information pertaining to the interactions between biological macromolecules and ligands is of potential significance for understanding of molecular mechanisms in key biological processes. Recently, nuclear magnetic resonance (NMR) spectroscopic techniques has come of age and has widened its scope to characterize binding interactions of small molecules with biological macromolecules especially, proteins. NMR spectroscopy-based techniques are versatile due to their ability to examine weak binding interactions and for rapid screening the binding affinities of ligands with proteins at atomic resolution. In this review, we provide a broad overview of some of the important NMR approaches to investigate interactions of small organic molecules with proteins.

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Research Organization:: Univ. of Arkansas, Fayetteville, AR (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-01ER15161

OSTI ID:: 1609352

Journal Information:: Natural Product Communications, Vol. 14, Issue 5; ISSN 1934-578X

Publisher:: SAGECopyright Statement

Country of Publication:: United States

Language:: English

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