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Title: An algal enzyme required for biosynthesis of the most abundant marine carotenoids

Abstract

Fucoxanthin and its derivatives are the main light-harvesting pigments in the photosynthetic apparatus of many chromalveolate algae and represent the most abundant carotenoids in the world’s oceans, thus being major facilitators of marine primary production. A central step in fucoxanthin biosynthesis that has been elusive so far is the conversion of violaxanthin to neoxanthin. Here, we show that in chromalveolates, this reaction is catalyzed by violaxanthin de-epoxidase–like (VDL) proteins and that VDL is also involved in the formation of other light-harvesting carotenoids such as peridinin or vaucheriaxanthin. VDL is closely related to the photoprotective enzyme violaxanthin de-epoxidase that operates in plants and most algae, revealing that in major phyla of marine algae, an ancient gene duplication triggered the evolution of carotenoid functions beyond photoprotection toward light harvesting.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [4];  [4]; ORCiD logo [4]; ORCiD logo [4];  [4];  [4]; ORCiD logo [3]; ORCiD logo [5]; ORCiD logo [4]
  1. Johannes Gutenberg Univ., Mainz (Germany). Inst. für Molekulare Physiologie, Pflanzenbiochemi
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
  3. Univ. of California, Berkeley, CA (United States)
  4. Johannes Gutenberg Univ., Mainz (Germany). Inst. für Molekulare Physiologie, Pflanzenbiochemi
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States). Howard Hughes Medical Inst.
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; Danish Council for Independent Research
OSTI Identifier:
1609117
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Science Advances
Additional Journal Information:
Journal Volume: 6; Journal Issue: 10; Journal ID: ISSN 2375-2548
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dautermann, O., Lyska, D., Andersen-Ranberg, J., Becker, M., Fröhlich-Nowoisky, J., Gartmann, H., Krämer, L. C., Mayr, K., Pieper, D., Rij, L. M., Wipf, H. M. -L., Niyogi, K. K., and Lohr, M. An algal enzyme required for biosynthesis of the most abundant marine carotenoids. United States: N. p., 2020. Web. https://doi.org/10.1126/sciadv.aaw9183.
Dautermann, O., Lyska, D., Andersen-Ranberg, J., Becker, M., Fröhlich-Nowoisky, J., Gartmann, H., Krämer, L. C., Mayr, K., Pieper, D., Rij, L. M., Wipf, H. M. -L., Niyogi, K. K., & Lohr, M. An algal enzyme required for biosynthesis of the most abundant marine carotenoids. United States. https://doi.org/10.1126/sciadv.aaw9183
Dautermann, O., Lyska, D., Andersen-Ranberg, J., Becker, M., Fröhlich-Nowoisky, J., Gartmann, H., Krämer, L. C., Mayr, K., Pieper, D., Rij, L. M., Wipf, H. M. -L., Niyogi, K. K., and Lohr, M. Wed . "An algal enzyme required for biosynthesis of the most abundant marine carotenoids". United States. https://doi.org/10.1126/sciadv.aaw9183. https://www.osti.gov/servlets/purl/1609117.
@article{osti_1609117,
title = {An algal enzyme required for biosynthesis of the most abundant marine carotenoids},
author = {Dautermann, O. and Lyska, D. and Andersen-Ranberg, J. and Becker, M. and Fröhlich-Nowoisky, J. and Gartmann, H. and Krämer, L. C. and Mayr, K. and Pieper, D. and Rij, L. M. and Wipf, H. M. -L. and Niyogi, K. K. and Lohr, M.},
abstractNote = {Fucoxanthin and its derivatives are the main light-harvesting pigments in the photosynthetic apparatus of many chromalveolate algae and represent the most abundant carotenoids in the world’s oceans, thus being major facilitators of marine primary production. A central step in fucoxanthin biosynthesis that has been elusive so far is the conversion of violaxanthin to neoxanthin. Here, we show that in chromalveolates, this reaction is catalyzed by violaxanthin de-epoxidase–like (VDL) proteins and that VDL is also involved in the formation of other light-harvesting carotenoids such as peridinin or vaucheriaxanthin. VDL is closely related to the photoprotective enzyme violaxanthin de-epoxidase that operates in plants and most algae, revealing that in major phyla of marine algae, an ancient gene duplication triggered the evolution of carotenoid functions beyond photoprotection toward light harvesting.},
doi = {10.1126/sciadv.aaw9183},
journal = {Science Advances},
number = 10,
volume = 6,
place = {United States},
year = {2020},
month = {3}
}

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