PMR5, an acetylation protein at the intersection of pectin biosynthesis and defense against fungal pathogens
Abstract
Powdery mildew (Golovinomyces cichoracearum), one of the most prolific obligate biotrophic fungal pathogens worldwide, infects its host by penetrating the plant cell wall without activating the plant's innate immune system. The Arabidopsis mutant powdery mildew resistant 5 (pmr5) carries a mutation in a putative pectin acetyltransferase gene that confers enhanced resistance to powdery mildew. In this work, we show that heterologously expressed PMR5 protein transfers acetyl groups from [14C]-acetyl-CoA to oligogalacturonides. Through site-directed mutagenesis, we show that three amino acids within a highly conserved esterase domain in putative PMR5 orthologs are necessary for PMR5 function. A suppressor screen of mutagenized pmr5 seed selecting for increased powdery mildew susceptibility identified two previously characterized genes affecting the acetylation of plant cell wall polysaccharides, RWA2 and TBR. The rwa2 and tbr mutants also suppress powdery mildew disease resistance in pmr6, a mutant defective in a putative pectate lyase gene. Cell wall analysis of pmr5 and pmr6, and their rwa2 and tbr suppressor mutants, demonstrates minor shifts in cellulose and pectin composition. In direct contrast to their increased powdery mildew resistance, both pmr5 and pmr6 plants are highly susceptibile to multiple strains of the generalist necrotroph Botrytis cinerea, and have decreased camalexin production upon infectionmore »
- Authors:
-
[1];
[1];
[4];
[5];
- Univ. of California, Berkeley, CA (United States); Energy Biosciences Inst., Berkeley, CA (United States)
- Univ. of California, Davis, CA (United States)
- Joint BioEnergy Inst., Emeryville, CA (United States)
- Univ. of California, Berkeley, CA (United States); ; USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Univ. of California, Berkeley, CA (United States); Joint BioEnergy Inst., Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Bioenergy Research Centers (BRC) (United States). Joint BioEnergy Inst. (JBEI); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); Energy Biosciences Institute; National Science Foundation (NSF)
- OSTI Identifier:
- 1609109
- Alternate Identifier(s):
- OSTI ID: 1575952
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Volume: 100; Journal Issue: 5; Journal ID: ISSN 0960-7412
- Publisher:
- Society for Experimental Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; host resistance; cell wall biochemistry; fungal disease; powdery mildew; pectin acetylation; cell wall fragments
Citation Formats
Chiniquy, Dawn, Underwood, William, Corwin, Jason, Ryan, Andrew, Szemenyei, Heidi, Lim, Candice C., Stonebloom, Solomon H., Birdseye, Devon S., Vogel, John, Kliebenstein, Daniel, Scheller, Henrik V., and Somerville, Shauna. PMR5, an acetylation protein at the intersection of pectin biosynthesis and defense against fungal pathogens. United States: N. p., 2019.
Web. doi:10.1111/tpj.14497.
Chiniquy, Dawn, Underwood, William, Corwin, Jason, Ryan, Andrew, Szemenyei, Heidi, Lim, Candice C., Stonebloom, Solomon H., Birdseye, Devon S., Vogel, John, Kliebenstein, Daniel, Scheller, Henrik V., & Somerville, Shauna. PMR5, an acetylation protein at the intersection of pectin biosynthesis and defense against fungal pathogens. United States. https://doi.org/10.1111/tpj.14497
Chiniquy, Dawn, Underwood, William, Corwin, Jason, Ryan, Andrew, Szemenyei, Heidi, Lim, Candice C., Stonebloom, Solomon H., Birdseye, Devon S., Vogel, John, Kliebenstein, Daniel, Scheller, Henrik V., and Somerville, Shauna. Wed .
"PMR5, an acetylation protein at the intersection of pectin biosynthesis and defense against fungal pathogens". United States. https://doi.org/10.1111/tpj.14497. https://www.osti.gov/servlets/purl/1609109.
@article{osti_1609109,
title = {PMR5, an acetylation protein at the intersection of pectin biosynthesis and defense against fungal pathogens},
author = {Chiniquy, Dawn and Underwood, William and Corwin, Jason and Ryan, Andrew and Szemenyei, Heidi and Lim, Candice C. and Stonebloom, Solomon H. and Birdseye, Devon S. and Vogel, John and Kliebenstein, Daniel and Scheller, Henrik V. and Somerville, Shauna},
abstractNote = {Powdery mildew (Golovinomyces cichoracearum), one of the most prolific obligate biotrophic fungal pathogens worldwide, infects its host by penetrating the plant cell wall without activating the plant's innate immune system. The Arabidopsis mutant powdery mildew resistant 5 (pmr5) carries a mutation in a putative pectin acetyltransferase gene that confers enhanced resistance to powdery mildew. In this work, we show that heterologously expressed PMR5 protein transfers acetyl groups from [14C]-acetyl-CoA to oligogalacturonides. Through site-directed mutagenesis, we show that three amino acids within a highly conserved esterase domain in putative PMR5 orthologs are necessary for PMR5 function. A suppressor screen of mutagenized pmr5 seed selecting for increased powdery mildew susceptibility identified two previously characterized genes affecting the acetylation of plant cell wall polysaccharides, RWA2 and TBR. The rwa2 and tbr mutants also suppress powdery mildew disease resistance in pmr6, a mutant defective in a putative pectate lyase gene. Cell wall analysis of pmr5 and pmr6, and their rwa2 and tbr suppressor mutants, demonstrates minor shifts in cellulose and pectin composition. In direct contrast to their increased powdery mildew resistance, both pmr5 and pmr6 plants are highly susceptibile to multiple strains of the generalist necrotroph Botrytis cinerea, and have decreased camalexin production upon infection with B. cinerea. These results illustrate that cell wall composition is intimately connected to fungal disease resistance and outline a potential route for engineering powdery mildew resistance into susceptible crop species.},
doi = {10.1111/tpj.14497},
journal = {The Plant Journal},
number = 5,
volume = 100,
place = {United States},
year = {2019},
month = {8}
}
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