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Title: A Phytochrome B-Independent Pathway Restricts Growth at High Levels of Jasmonate Defense

Abstract

The plant hormone jasmonate (JA) promotes resistance to biotic stress by stimulating the degradation of JASMONATE ZIMDOMAIN (JAZ) proteins, which relieves repression on MYC transcription factors that execute defense programs. JA-triggered depletion of JAZ proteins in Arabidopsis (Arabidopsis thaliana) is also associated with reduced growth and seed production, but the mechanisms underlying these pleiotropic growth effects remain unclear. Here, we investigated this question using an Arabidopsis JAZ-deficient mutant (jazD; jaz1–jaz7, jaz9, jaz10, and jaz13) that exhibits high levels of defense and strong growth inhibition. Genetic suppressor screens for mutations that uncouple growth-defense tradeoffs in the jazD mutant identified nine independent causal mutations in the red-light receptor phytochrome B (phyB). Unlike the ability of the phyB mutations to completely uncouple the mild growth-defense phenotypes in a jaz mutant (jazQ) defective in JAZ1, JAZ3, JAZ4, JAZ9, and JAZ10, phyB null alleles only weakly alleviated the growth and reproductive defects in the jazD mutant. phyB-independent growth restriction of the jazD mutant was tightly correlated with upregulation of the Trp biosynthetic pathway but not with changes in central carbon metabolism. Interestingly, jazD and jazD phyB plants were insensitive to a chemical inhibitor of Trp biosynthesis, which is a phenotype previously observed in plants expressingmore » hyperactive MYC transcription factors that cannot bind JAZ repressors. These data provide evidence that the mechanisms underlying JA-mediated growth-defense balance depend on the level of defense, and they further establish an association between growth inhibition at high levels of defense and dysregulation of Trp biosynthesis.« less

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [5]
  1. Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824
  2. Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824
  3. Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, Plant Resilience Institute, Michigan State University, East Lansing, Michigan 42284
  4. Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824
  5. Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, Plant Resilience Institute, Michigan State University, East Lansing, Michigan 42284
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1608185
Alternate Identifier(s):
OSTI ID: 1735822
Grant/Contract Number:  
FG02-91ER20021
Resource Type:
Published Article
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Name: Plant Physiology (Bethesda) Journal Volume: 183 Journal Issue: 2; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Major, Ian T., Guo, Qiang, Zhai, Jinling, Kapali, George, Kramer, David M., and Howe, Gregg A. A Phytochrome B-Independent Pathway Restricts Growth at High Levels of Jasmonate Defense. United States: N. p., 2020. Web. doi:10.1104/pp.19.01335.
Major, Ian T., Guo, Qiang, Zhai, Jinling, Kapali, George, Kramer, David M., & Howe, Gregg A. A Phytochrome B-Independent Pathway Restricts Growth at High Levels of Jasmonate Defense. United States. https://doi.org/10.1104/pp.19.01335
Major, Ian T., Guo, Qiang, Zhai, Jinling, Kapali, George, Kramer, David M., and Howe, Gregg A. Fri . "A Phytochrome B-Independent Pathway Restricts Growth at High Levels of Jasmonate Defense". United States. https://doi.org/10.1104/pp.19.01335.
@article{osti_1608185,
title = {A Phytochrome B-Independent Pathway Restricts Growth at High Levels of Jasmonate Defense},
author = {Major, Ian T. and Guo, Qiang and Zhai, Jinling and Kapali, George and Kramer, David M. and Howe, Gregg A.},
abstractNote = {The plant hormone jasmonate (JA) promotes resistance to biotic stress by stimulating the degradation of JASMONATE ZIMDOMAIN (JAZ) proteins, which relieves repression on MYC transcription factors that execute defense programs. JA-triggered depletion of JAZ proteins in Arabidopsis (Arabidopsis thaliana) is also associated with reduced growth and seed production, but the mechanisms underlying these pleiotropic growth effects remain unclear. Here, we investigated this question using an Arabidopsis JAZ-deficient mutant (jazD; jaz1–jaz7, jaz9, jaz10, and jaz13) that exhibits high levels of defense and strong growth inhibition. Genetic suppressor screens for mutations that uncouple growth-defense tradeoffs in the jazD mutant identified nine independent causal mutations in the red-light receptor phytochrome B (phyB). Unlike the ability of the phyB mutations to completely uncouple the mild growth-defense phenotypes in a jaz mutant (jazQ) defective in JAZ1, JAZ3, JAZ4, JAZ9, and JAZ10, phyB null alleles only weakly alleviated the growth and reproductive defects in the jazD mutant. phyB-independent growth restriction of the jazD mutant was tightly correlated with upregulation of the Trp biosynthetic pathway but not with changes in central carbon metabolism. Interestingly, jazD and jazD phyB plants were insensitive to a chemical inhibitor of Trp biosynthesis, which is a phenotype previously observed in plants expressing hyperactive MYC transcription factors that cannot bind JAZ repressors. These data provide evidence that the mechanisms underlying JA-mediated growth-defense balance depend on the level of defense, and they further establish an association between growth inhibition at high levels of defense and dysregulation of Trp biosynthesis.},
doi = {10.1104/pp.19.01335},
journal = {Plant Physiology (Bethesda)},
number = 2,
volume = 183,
place = {United States},
year = {2020},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1104/pp.19.01335

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