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Title: Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production

Authors:
; ORCiD logo; ORCiD logo; ; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E)
OSTI Identifier:
1608159
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Metabolic Engineering
Additional Journal Information:
Journal Name: Metabolic Engineering Journal Volume: 60 Journal Issue: C; Journal ID: ISSN 1096-7176
Publisher:
Elsevier
Country of Publication:
Belgium
Language:
English

Citation Formats

Bennett, R. Kyle, Dillon, Michael, Gerald Har, Jie Ren, Agee, Alec, von Hagel, Bryan, Rohlhill, Julia, Antoniewicz, Maciek R., and Papoutsakis, Eleftherios T. Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production. Belgium: N. p., 2020. Web. https://doi.org/10.1016/j.ymben.2020.03.003.
Bennett, R. Kyle, Dillon, Michael, Gerald Har, Jie Ren, Agee, Alec, von Hagel, Bryan, Rohlhill, Julia, Antoniewicz, Maciek R., & Papoutsakis, Eleftherios T. Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production. Belgium. https://doi.org/10.1016/j.ymben.2020.03.003
Bennett, R. Kyle, Dillon, Michael, Gerald Har, Jie Ren, Agee, Alec, von Hagel, Bryan, Rohlhill, Julia, Antoniewicz, Maciek R., and Papoutsakis, Eleftherios T. Wed . "Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production". Belgium. https://doi.org/10.1016/j.ymben.2020.03.003.
@article{osti_1608159,
title = {Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production},
author = {Bennett, R. Kyle and Dillon, Michael and Gerald Har, Jie Ren and Agee, Alec and von Hagel, Bryan and Rohlhill, Julia and Antoniewicz, Maciek R. and Papoutsakis, Eleftherios T.},
abstractNote = {},
doi = {10.1016/j.ymben.2020.03.003},
journal = {Metabolic Engineering},
number = C,
volume = 60,
place = {Belgium},
year = {2020},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1016/j.ymben.2020.03.003

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Cited by: 1 work
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Works referenced in this record:

Engineering the bioconversion of methane and methanol to fuels and chemicals in native and synthetic methylotrophs
journal, April 2018


Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization
journal, June 2015

  • Whitaker, William B.; Sandoval, Nicholas R.; Bennett, Robert K.
  • Current Opinion in Biotechnology, Vol. 33
  • DOI: 10.1016/j.copbio.2015.01.007

Quantifying Reductive Carboxylation Flux of Glutamine to Lipid in a Brown Adipocyte Cell Line
journal, March 2008

  • Yoo, Hyuntae; Antoniewicz, Maciek R.; Stephanopoulos, Gregory
  • Journal of Biological Chemistry, Vol. 283, Issue 30
  • DOI: 10.1074/jbc.M706494200

Parallel labeling experiments for pathway elucidation and 13C metabolic flux analysis
journal, December 2015


Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production
journal, September 2018


Methanol assimilation in Escherichia coli is improved by co-utilization of threonine and deletion of leucine-responsive regulatory protein
journal, January 2018


Identification of the cpdA Gene Encoding Cyclic 3ʹ,5ʹ-Adenosine Monophosphate Phosphodiesterase in Escherichia coli
journal, October 1996

  • Imamura, Ryu; Yamanaka, Kunitoshi; Ogura, Teru
  • Journal of Biological Chemistry, Vol. 271, Issue 41
  • DOI: 10.1074/jbc.271.41.25423

13C metabolic flux analysis of microbial and mammalian systems is enhanced with GC-MS measurements of glycogen and RNA labeling
journal, November 2016


Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate
journal, August 2015

  • Leßmeier, Lennart; Pfeifenschneider, Johannes; Carnicer, Marc
  • Applied Microbiology and Biotechnology, Vol. 99, Issue 23
  • DOI: 10.1007/s00253-015-6906-5

Metabolic Engineering of Corynebacterium glutamicum for Methanol Metabolism
journal, January 2015

  • Witthoff, Sabrina; Schmitz, Katja; Niedenführ, Sebastian
  • Applied and Environmental Microbiology, Vol. 81, Issue 6, p. 2215-2225
  • DOI: 10.1128/AEM.03110-14

l-Ribulose production by an Escherichia coli harboring l-arabinose isomerase from Bacillus licheniformis
journal, May 2010

  • Zhang, Ye-Wang; Jeya, Marimuthu; Lee, Jung-Kul
  • Applied Microbiology and Biotechnology, Vol. 87, Issue 6
  • DOI: 10.1007/s00253-010-2600-9

High-resolution 13C metabolic flux analysis
journal, August 2019


ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli
journal, May 2012

  • Xu, Peng; Vansiri, Amerin; Bhan, Namita
  • ACS Synthetic Biology, Vol. 1, Issue 7
  • DOI: 10.1021/sb300016b

Elementary metabolite units (EMU): A novel framework for modeling isotopic distributions
journal, January 2007

  • Antoniewicz, Maciek R.; Kelleher, Joanne K.; Stephanopoulos, Gregory
  • Metabolic Engineering, Vol. 9, Issue 1
  • DOI: 10.1016/j.ymben.2006.09.001

Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae
journal, December 2017


Rethinking biological activation of methane and conversion to liquid fuels
journal, April 2014


Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol
journal, May 2017

  • Rohlhill, Julia; Sandoval, Nicholas R.; Papoutsakis, Eleftherios T.
  • ACS Synthetic Biology, Vol. 6, Issue 8, p. 1584-1595
  • DOI: 10.1021/acssynbio.7b00114

Enzyme I facilitates reverse flux from pyruvate to phosphoenolpyruvate in Escherichia coli
journal, January 2017

  • Long, Christopher P.; Au, Jennifer; Sandoval, Nicholas R.
  • Nature Communications, Vol. 8, Issue 1
  • DOI: 10.1038/ncomms14316

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection
journal, February 2006

  • Baba, Tomoya; Ara, Takeshi; Hasegawa, Miki
  • Molecular Systems Biology, Vol. 2, Article No. 2006.0008
  • DOI: 10.1038/msb4100050

Synthetic methanol auxotrophy of Escherichia coli for methanol-dependent growth and production
journal, September 2018


Characterization of physiological responses to 22 gene knockouts in Escherichia coli central carbon metabolism
journal, September 2016


Improving formaldehyde consumption drives methanol assimilation in engineered E. coli
journal, June 2018


Engineering Escherichia coli for methanol conversion
journal, March 2015


Genetic Basis of Growth Adaptation of Escherichia coli after Deletion of pgi, a Major Metabolic Gene
journal, November 2010


Quantifying Biomass Composition by Gas Chromatography/Mass Spectrometry
journal, September 2014

  • Long, Christopher P.; Antoniewicz, Maciek R.
  • Analytical Chemistry, Vol. 86, Issue 19
  • DOI: 10.1021/ac502734e

Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions by 13C metabolic flux analysis
journal, January 2017

  • Gonzalez, Jacqueline E.; Long, Christopher P.; Antoniewicz, Maciek R.
  • Metabolic Engineering, Vol. 39
  • DOI: 10.1016/j.ymben.2016.11.003

Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme in Escherichia coli
journal, December 2017

  • Long, Christopher P.; Gonzalez, Jacqueline E.; Feist, Adam M.
  • Proceedings of the National Academy of Sciences, Vol. 115, Issue 1
  • DOI: 10.1073/pnas.1716056115

Expression of Clostridium acetobutylicumATCC 824 Genes in Escherichia coli for Acetone Production and Acetate Detoxification
journal, March 1998

  • Bermejo, Lourdes L.; Welker, Neil E.; Papoutsakis, Eleftherios T.
  • Applied and Environmental Microbiology, Vol. 64, Issue 3
  • DOI: 10.1128/AEM.64.3.1079-1085.1998

A modified pathway for the production of acetone in Escherichia coli
journal, January 2013


Comparison of individual component deletions in a glucose-specific phosphotransferase system revealed their different applications
journal, August 2015

  • Liang, Quanfeng; Zhang, Fengyu; Li, Yikui
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep13200

Use of the isocitrate dehydrogenase structural gene for attachment of e14 in Escherichia coli K-12.
journal, January 1989


Measuring the Composition and Stable-Isotope Labeling of Algal Biomass Carbohydrates via Gas Chromatography/Mass Spectrometry
journal, April 2016


Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production
journal, October 2019


One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000

  • Datsenko, K. A.; Wanner, B. L.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
  • DOI: 10.1073/pnas.120163297

Deletion of four genes in Escherichia coli enables preferential consumption of xylose and secretion of glucose
journal, March 2019


Scaffoldless engineered enzyme assembly for enhanced methanol utilization
journal, October 2016

  • Price, J. Vincent; Chen, Long; Whitaker, W. Brian
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 45
  • DOI: 10.1073/pnas.1601797113

Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli
journal, January 2017


Methanol-essential growth of Escherichia coli
journal, April 2018


Improving synthetic methylotrophy via dynamic formaldehyde regulation of pentose phosphate pathway genes and redox perturbation
journal, January 2020


Pathway engineering for the production of aromatic compounds in Escherichia coli
journal, May 1996

  • Floras, Noemí; Xiao, Jimmy; Berry, Alan
  • Nature Biotechnology, Vol. 14, Issue 5
  • DOI: 10.1038/nbt0596-620