Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production

Bennett, R. Kyle; Dillon, Michael; Gerald Har, Jie Ren; Agee, Alec; von Hagel, Bryan; Rohlhill, Julia; Antoniewicz, Maciek R.; Papoutsakis, Eleftherios T.

doi:10.1016/j.ymben.2020.03.003

Title: Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production

Authors:

Bennett, R. Kyle;

;

; Agee, Alec; von Hagel, Bryan; Rohlhill, Julia; Antoniewicz, Maciek R.; Papoutsakis, Eleftherios T.

Publication Date:: 2020-07-01

Sponsoring Org.:: USDOE Advanced Research Projects Agency - Energy (ARPA-E)

OSTI Identifier:: 1608159

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Metabolic Engineering

Additional Journal Information:: Journal Name: Metabolic Engineering Journal Volume: 60 Journal Issue: C; Journal ID: ISSN 1096-7176

Publisher:: Elsevier

Country of Publication:: Belgium

Language:: English

Citation Formats


                    Bennett, R. Kyle, Dillon, Michael, Gerald Har, Jie Ren, Agee, Alec, von Hagel, Bryan, Rohlhill, Julia, Antoniewicz, Maciek R., and Papoutsakis, Eleftherios T. Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production.  Belgium: N. p., 2020. 
Web.  doi:10.1016/j.ymben.2020.03.003.

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                    Bennett, R. Kyle, Dillon, Michael, Gerald Har, Jie Ren, Agee, Alec, von Hagel, Bryan, Rohlhill, Julia, Antoniewicz, Maciek R., & Papoutsakis, Eleftherios T. Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production.  Belgium.  https://doi.org/10.1016/j.ymben.2020.03.003

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                    Bennett, R. Kyle, Dillon, Michael, Gerald Har, Jie Ren, Agee, Alec, von Hagel, Bryan, Rohlhill, Julia, Antoniewicz, Maciek R., and Papoutsakis, Eleftherios T. Wed .  
"Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production".  Belgium.  https://doi.org/10.1016/j.ymben.2020.03.003.

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@article{osti_1608159,

  title        = {Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production},

  author       = {Bennett, R. Kyle and Dillon, Michael and Gerald Har, Jie Ren and Agee, Alec and von Hagel, Bryan and Rohlhill, Julia and Antoniewicz, Maciek R. and Papoutsakis, Eleftherios T.},

  abstractNote = {},

  doi          = {10.1016/j.ymben.2020.03.003},

  journal      = {Metabolic Engineering},

  number       = C,

  volume       = 60,

  place        = {Belgium},

  year         = {2020},

  month        = {7}

}

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https://doi.org/10.1016/j.ymben.2020.03.003

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Title: Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production

Citation Formats

Engineering the bioconversion of methane and methanol to fuels and chemicals in native and synthetic methylotrophs journal, April 2018

Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization journal, June 2015

Quantifying Reductive Carboxylation Flux of Glutamine to Lipid in a Brown Adipocyte Cell Line journal, March 2008

Parallel labeling experiments for pathway elucidation and 13C metabolic flux analysis journal, December 2015

Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph journal, January 2018

Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production journal, September 2018

Methanol assimilation in Escherichia coli is improved by co-utilization of threonine and deletion of leucine-responsive regulatory protein journal, January 2018

Identification of the cpdA Gene Encoding Cyclic 3ʹ,5ʹ-Adenosine Monophosphate Phosphodiesterase in Escherichia coli journal, October 1996

13C metabolic flux analysis of microbial and mammalian systems is enhanced with GC-MS measurements of glycogen and RNA labeling journal, November 2016

Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate journal, August 2015

Metabolic Engineering of Corynebacterium glutamicum for Methanol Metabolism journal, January 2015

l-Ribulose production by an Escherichia coli harboring l-arabinose isomerase from Bacillus licheniformis journal, May 2010

High-resolution 13C metabolic flux analysis journal, August 2019

ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli journal, May 2012

Elementary metabolite units (EMU): A novel framework for modeling isotopic distributions journal, January 2007

Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae journal, December 2017

Rethinking biological activation of methane and conversion to liquid fuels journal, April 2014

Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol journal, May 2017

Enzyme I facilitates reverse flux from pyruvate to phosphoenolpyruvate in Escherichia coli journal, January 2017

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection journal, February 2006

Synthetic methanol auxotrophy of Escherichia coli for methanol-dependent growth and production journal, September 2018

Characterization of physiological responses to 22 gene knockouts in Escherichia coli central carbon metabolism journal, September 2016

Improving formaldehyde consumption drives methanol assimilation in engineered E. coli journal, June 2018

Engineering Escherichia coli for methanol conversion journal, March 2015

Genetic Basis of Growth Adaptation of Escherichia coli after Deletion of pgi, a Major Metabolic Gene journal, November 2010

Analysis of Carbon Metabolism in Escherichia coli Strains with an Inactive Phosphotransferase System by 13C Labeling and NMR Spectroscopy journal, April 2002

Quantifying Biomass Composition by Gas Chromatography/Mass Spectrometry journal, September 2014

Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions by 13C metabolic flux analysis journal, January 2017

Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme in Escherichia coli journal, December 2017

Expression of Clostridium acetobutylicumATCC 824 Genes in Escherichia coli for Acetone Production and Acetate Detoxification journal, March 1998

A modified pathway for the production of acetone in Escherichia coli journal, January 2013

Comparison of individual component deletions in a glucose-specific phosphotransferase system revealed their different applications journal, August 2015

Use of the isocitrate dehydrogenase structural gene for attachment of e14 in Escherichia coli K-12. journal, January 1989

Measuring the Composition and Stable-Isotope Labeling of Algal Biomass Carbohydrates via Gas Chromatography/Mass Spectrometry journal, April 2016

Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production journal, October 2019

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products journal, May 2000

Deletion of four genes in Escherichia coli enables preferential consumption of xylose and secretion of glucose journal, March 2019

Scaffoldless engineered enzyme assembly for enhanced methanol utilization journal, October 2016

Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli journal, January 2017

Methanol-essential growth of Escherichia coli journal, April 2018

Improving synthetic methylotrophy via dynamic formaldehyde regulation of pentose phosphate pathway genes and redox perturbation journal, January 2020

Pathway engineering for the production of aromatic compounds in Escherichia coli journal, May 1996

Engineering the bioconversion of methane and methanol to fuels and chemicals in native and synthetic methylotrophs
journal, April 2018

Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization
journal, June 2015

Quantifying Reductive Carboxylation Flux of Glutamine to Lipid in a Brown Adipocyte Cell Line
journal, March 2008

Parallel labeling experiments for pathway elucidation and 13C metabolic flux analysis
journal, December 2015

Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph
journal, January 2018

Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production
journal, September 2018

Methanol assimilation in Escherichia coli is improved by co-utilization of threonine and deletion of leucine-responsive regulatory protein
journal, January 2018

Identification of the cpdA Gene Encoding Cyclic 3ʹ,5ʹ-Adenosine Monophosphate Phosphodiesterase in Escherichia coli
journal, October 1996

13C metabolic flux analysis of microbial and mammalian systems is enhanced with GC-MS measurements of glycogen and RNA labeling
journal, November 2016

Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate
journal, August 2015

Metabolic Engineering of Corynebacterium glutamicum for Methanol Metabolism
journal, January 2015

l-Ribulose production by an Escherichia coli harboring l-arabinose isomerase from Bacillus licheniformis
journal, May 2010

High-resolution 13C metabolic flux analysis
journal, August 2019

ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli
journal, May 2012

Elementary metabolite units (EMU): A novel framework for modeling isotopic distributions
journal, January 2007

Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae
journal, December 2017

Rethinking biological activation of methane and conversion to liquid fuels
journal, April 2014

Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol
journal, May 2017

Enzyme I facilitates reverse flux from pyruvate to phosphoenolpyruvate in Escherichia coli
journal, January 2017

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection
journal, February 2006

Synthetic methanol auxotrophy of Escherichia coli for methanol-dependent growth and production
journal, September 2018

Characterization of physiological responses to 22 gene knockouts in Escherichia coli central carbon metabolism
journal, September 2016

Improving formaldehyde consumption drives methanol assimilation in engineered E. coli
journal, June 2018

Engineering Escherichia coli for methanol conversion
journal, March 2015

Genetic Basis of Growth Adaptation of Escherichia coli after Deletion of pgi, a Major Metabolic Gene
journal, November 2010

Analysis of Carbon Metabolism in Escherichia coli Strains with an Inactive Phosphotransferase System by 13C Labeling and NMR Spectroscopy
journal, April 2002

Quantifying Biomass Composition by Gas Chromatography/Mass Spectrometry
journal, September 2014

Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions by 13C metabolic flux analysis
journal, January 2017

Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme in Escherichia coli
journal, December 2017

Expression of Clostridium acetobutylicumATCC 824 Genes in Escherichia coli for Acetone Production and Acetate Detoxification
journal, March 1998

A modified pathway for the production of acetone in Escherichia coli
journal, January 2013

Comparison of individual component deletions in a glucose-specific phosphotransferase system revealed their different applications
journal, August 2015

Use of the isocitrate dehydrogenase structural gene for attachment of e14 in Escherichia coli K-12.
journal, January 1989

Measuring the Composition and Stable-Isotope Labeling of Algal Biomass Carbohydrates via Gas Chromatography/Mass Spectrometry
journal, April 2016

Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production
journal, October 2019

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000

Deletion of four genes in Escherichia coli enables preferential consumption of xylose and secretion of glucose
journal, March 2019

Scaffoldless engineered enzyme assembly for enhanced methanol utilization
journal, October 2016

Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli
journal, January 2017

Methanol-essential growth of Escherichia coli
journal, April 2018

Improving synthetic methylotrophy via dynamic formaldehyde regulation of pentose phosphate pathway genes and redox perturbation
journal, January 2020

Pathway engineering for the production of aromatic compounds in Escherichia coli
journal, May 1996