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Title: Osteoporosis Treatments Affect Bone Matrix Maturation in a Rat Model of Induced Cortical Remodeling

Abstract

To test how osteoporosis drugs affect bone matrix maturation during cortical bone remodeling, 72 pregnant rats were switched from a 0.4% to a 0.01% calcium diet at parturition for a 23-day lactation period. At weaning, eight dams were sacrificed to establish baseline values, while the remaining dams were returned to 0.4% calcium and treated with vehicle (saline), sodium fluoride (NaF), zoledronic acid (ZA), or sclerostin antibody (Scl-Ab) for either 7 or 28 days (eight animals per group per time point). Femora were examined by μCT, dynamic histomorphometry, Fourier transform infrared imaging, and three-point bending of notched specimens. Cortical porosity decreased in all groups from baseline to day 28. Intracortical mineralizing surface (MS/BS) and mineral apposition rate (MAR), as well as the mineral-to-matrix ratio were unaffected by treatment, but intracortical crystallinity was increased in the ZA group at day 10 compared with vehicle. Cortical area increased in all groups over 28 days mainly because of an addition of bone at the endocortical surface. Endocortical MS/BS did not vary among the groups, but endocortical MAR was suppressed in the NaF group at day 2 and elevated in the Scl-Ab group at day 4 compared with vehicle. Endocortical mineral-to-matrix ratio was increased atmore » days 5 and 10 following NaF treatment and endocortical crystallinity was increased at day 5 following ZA treatment compared with vehicle. Fracture toughness did not differ among the groups. Thus, the treatments affected matrix maturation more strongly at the endocortical then intracortical envelope. In this model of induced remodeling, the bone formation phase is synchronized at multiple sites, facilitating study of the effects of drugs or other bone-targeting agents on matrix maturation independent of their effects on the initiation of remodeling.« less

Authors:
ORCiD logo [1];  [2];  [2];  [3]; ORCiD logo [3]; ORCiD logo [3];  [1]
  1. Department of Cell &, Molecular Medicine Rush University Medical Center Chicago IL USA, Department of Orthopedic Surgery Rush University Medical Center Chicago IL USA
  2. Department of Cell &, Molecular Medicine Rush University Medical Center Chicago IL USA
  3. Clinical Cell Biology, Research Unit of Pathology, Department of Clinical Research University of Southern Denmark, and Department of Pathology, Odense University Hospital Odense Denmark, Department of Molecular Medicine University of Southern Denmark Odense Denmark
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1608144
Alternate Identifier(s):
OSTI ID: 1608145; OSTI ID: 1629976
Grant/Contract Number:  
DE‐AC02‐05CH11231; AC02-05CH11231
Resource Type:
Published Article
Journal Name:
JBMR Plus
Additional Journal Information:
Journal Name: JBMR Plus Journal Volume: 4 Journal Issue: 4; Journal ID: ISSN 2473-4039
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
Country unknown/Code not available
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; BISPHOSPHONATE; BONE REMODELING; INTRACORTICAL; LACTATION; MINERALIZATION; SCLEROSTIN

Citation Formats

Ross, Ryan D., Anderson, Kyle, Davison, Reid, El‐Masri, Bilal M., Andreasen, Christina M., Andersen, Thomas L., and Sumner, Dale R. Osteoporosis Treatments Affect Bone Matrix Maturation in a Rat Model of Induced Cortical Remodeling. Country unknown/Code not available: N. p., 2020. Web. https://doi.org/10.1002/jbm4.10344.
Ross, Ryan D., Anderson, Kyle, Davison, Reid, El‐Masri, Bilal M., Andreasen, Christina M., Andersen, Thomas L., & Sumner, Dale R. Osteoporosis Treatments Affect Bone Matrix Maturation in a Rat Model of Induced Cortical Remodeling. Country unknown/Code not available. https://doi.org/10.1002/jbm4.10344
Ross, Ryan D., Anderson, Kyle, Davison, Reid, El‐Masri, Bilal M., Andreasen, Christina M., Andersen, Thomas L., and Sumner, Dale R. Thu . "Osteoporosis Treatments Affect Bone Matrix Maturation in a Rat Model of Induced Cortical Remodeling". Country unknown/Code not available. https://doi.org/10.1002/jbm4.10344.
@article{osti_1608144,
title = {Osteoporosis Treatments Affect Bone Matrix Maturation in a Rat Model of Induced Cortical Remodeling},
author = {Ross, Ryan D. and Anderson, Kyle and Davison, Reid and El‐Masri, Bilal M. and Andreasen, Christina M. and Andersen, Thomas L. and Sumner, Dale R.},
abstractNote = {To test how osteoporosis drugs affect bone matrix maturation during cortical bone remodeling, 72 pregnant rats were switched from a 0.4% to a 0.01% calcium diet at parturition for a 23-day lactation period. At weaning, eight dams were sacrificed to establish baseline values, while the remaining dams were returned to 0.4% calcium and treated with vehicle (saline), sodium fluoride (NaF), zoledronic acid (ZA), or sclerostin antibody (Scl-Ab) for either 7 or 28 days (eight animals per group per time point). Femora were examined by μCT, dynamic histomorphometry, Fourier transform infrared imaging, and three-point bending of notched specimens. Cortical porosity decreased in all groups from baseline to day 28. Intracortical mineralizing surface (MS/BS) and mineral apposition rate (MAR), as well as the mineral-to-matrix ratio were unaffected by treatment, but intracortical crystallinity was increased in the ZA group at day 10 compared with vehicle. Cortical area increased in all groups over 28 days mainly because of an addition of bone at the endocortical surface. Endocortical MS/BS did not vary among the groups, but endocortical MAR was suppressed in the NaF group at day 2 and elevated in the Scl-Ab group at day 4 compared with vehicle. Endocortical mineral-to-matrix ratio was increased at days 5 and 10 following NaF treatment and endocortical crystallinity was increased at day 5 following ZA treatment compared with vehicle. Fracture toughness did not differ among the groups. Thus, the treatments affected matrix maturation more strongly at the endocortical then intracortical envelope. In this model of induced remodeling, the bone formation phase is synchronized at multiple sites, facilitating study of the effects of drugs or other bone-targeting agents on matrix maturation independent of their effects on the initiation of remodeling.},
doi = {10.1002/jbm4.10344},
journal = {JBMR Plus},
number = 4,
volume = 4,
place = {Country unknown/Code not available},
year = {2020},
month = {2}
}

Journal Article:
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https://doi.org/10.1002/jbm4.10344

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