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Title: Raman spectroscopy coupled with ambient ionization mass spectrometry: A forensic laboratory investigation into rapid and simple dual instrumental analysis techniques

Abstract

We present a laboratory experiment, developed around a pair of instrumental analysis techniques, Raman spectroscopy and ambient ionization mass spectrometry (MS), conducted by senior chemistry undergraduate students for bulk analysis of over-the-counter drugs using benchtop versions of the two instruments, as well as trace analysis of illicit drugs utilizing the corresponding portable instruments. The identification and confirmation of seized drugs is a routine operation in forensic laboratories that can be simplified using these two instrumental techniques in tandem. Bulk samples are readily analyzed using conventional Raman spectroscopy followed by paper cone spray ionization (PCSI) MS, while trace analysis is allowed by a simple single-substrate dual-analyzer method using surface enhanced Raman spectroscopy (SERS) from paper followed by paper spray ionization (PSI) MS. The introduction of these novel strategies to the students exposed them to potential career paths in forensic science by means of a hands-on experience that provided fundamental knowledge about this set of analytical methods, including intercomparisons of their performance, as well as practical issues like costs, regulations and databases. The use of portable instrumentation represents an additional learning experience for the students, considering that it is cutting-edge technology that is just now being transferred from academic to industrial labsmore » and that it allows a valuable discussion of the advantages and disadvantages of bringing the laboratory to the field.« less

Authors:
 [1];  [2];  [2];  [2]
+ Show Author Affiliations
  1. Purdue Univ., West Lafayette, IN (United States); United States Navy Naval Air Systems Command (NAVAIR), China Lake, CA (United States)
  2. Purdue Univ., West Lafayette, IN (United States)
Publication Date:
Research Org.:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1800344
Alternate Identifier(s):
OSTI ID: 1607942
Grant/Contract Number:  
FG02-06ER15807
Resource Type:
Accepted Manuscript
Journal Name:
International Journal of Mass Spectrometry
Additional Journal Information:
Journal Volume: 452; Journal ID: ISSN 1387-3806
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; forensic analysis; laboratory instruction; hands-on learning; Raman spectroscopy; paper surface enhanced Raman spectroscopy; mass spectrometry; paper spray ionization; paper cone spray ionization; ambient ionization; drugs of abuse

Citation Formats

Fedick, Patrick W., Morato, Nicolás M., Pu, Fan, and Cooks, R. Graham. Raman spectroscopy coupled with ambient ionization mass spectrometry: A forensic laboratory investigation into rapid and simple dual instrumental analysis techniques. United States: N. p., 2020. Web. doi:10.1016/j.ijms.2020.116326.
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Fedick, Patrick W., Morato, Nicolás M., Pu, Fan, & Cooks, R. Graham. Raman spectroscopy coupled with ambient ionization mass spectrometry: A forensic laboratory investigation into rapid and simple dual instrumental analysis techniques. United States. https://doi.org/10.1016/j.ijms.2020.116326
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Fedick, Patrick W., Morato, Nicolás M., Pu, Fan, and Cooks, R. Graham. Tue . "Raman spectroscopy coupled with ambient ionization mass spectrometry: A forensic laboratory investigation into rapid and simple dual instrumental analysis techniques". United States. https://doi.org/10.1016/j.ijms.2020.116326. https://www.osti.gov/servlets/purl/1800344.
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@article{osti_1800344,
title = {Raman spectroscopy coupled with ambient ionization mass spectrometry: A forensic laboratory investigation into rapid and simple dual instrumental analysis techniques},
author = {Fedick, Patrick W. and Morato, Nicolás M. and Pu, Fan and Cooks, R. Graham},
abstractNote = {We present a laboratory experiment, developed around a pair of instrumental analysis techniques, Raman spectroscopy and ambient ionization mass spectrometry (MS), conducted by senior chemistry undergraduate students for bulk analysis of over-the-counter drugs using benchtop versions of the two instruments, as well as trace analysis of illicit drugs utilizing the corresponding portable instruments. The identification and confirmation of seized drugs is a routine operation in forensic laboratories that can be simplified using these two instrumental techniques in tandem. Bulk samples are readily analyzed using conventional Raman spectroscopy followed by paper cone spray ionization (PCSI) MS, while trace analysis is allowed by a simple single-substrate dual-analyzer method using surface enhanced Raman spectroscopy (SERS) from paper followed by paper spray ionization (PSI) MS. The introduction of these novel strategies to the students exposed them to potential career paths in forensic science by means of a hands-on experience that provided fundamental knowledge about this set of analytical methods, including intercomparisons of their performance, as well as practical issues like costs, regulations and databases. The use of portable instrumentation represents an additional learning experience for the students, considering that it is cutting-edge technology that is just now being transferred from academic to industrial labs and that it allows a valuable discussion of the advantages and disadvantages of bringing the laboratory to the field.},
doi = {10.1016/j.ijms.2020.116326},
journal = {International Journal of Mass Spectrometry},
number = ,
volume = 452,
place = {United States},
year = {Tue Mar 24 00:00:00 EDT 2020},
month = {Tue Mar 24 00:00:00 EDT 2020}
}
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https://doi.org/10.1016/j.ijms.2020.116326
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