A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures

Holler, Mirko; Ihli, Johannes; Tsai, Esther H.  R.; Nudelman, Fabio; Verezhak, Mariana; van de Berg, Wilma D.  J.; Shahmoradian, Sarah H.

doi:10.1107/S1600577519017028

Title: A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures

Journal Article · 29 January 2020 · Journal of Synchrotron Radiation (Online)

DOI: https://doi.org/10.1107/S1600577519017028 · OSTI ID:1607717

^[1]; Ihli, Johannes ^[1]; Tsai, Esther H. R. ^[2];

^[3]; Verezhak, Mariana ^[1]; van de Berg, Wilma D. J. ^[4]; Shahmoradian, Sarah H. ^[1]

Paul Scherrer Inst. (PSI), Villigen (Switzerland)
Brookhaven National Lab. (BNL), Upton, NY (United States)
Univ. of Edinburgh, Scotland (United Kingdom)
Amsterdam UMC, VU University Medical Center (the Netherlands)

A simple two-spindle based lathe system for the preparation of cylindrical samples intended for X-ray tomography is presented. The setup can operate at room temperature as well as under cryogenic conditions, allowing the preparation of samples down to 20 and 50 µm in diameter, respectively, within minutes. Case studies are presented involving the preparation of a brittle biomineral brachiopod shell and cryogenically fixed soft brain tissue, and their examination by means of ptychographic X-ray computed tomography reveals the preparation method to be mainly free from causing artefacts. Since this lathe system easily yields near-cylindrical samples ideal for tomography, a usage for a wide variety of otherwise challenging specimens is anticipated, in addition to potential use as a time- and cost-saving tool prior to focused ion-beam milling. Fast sample preparation becomes especially important in relation to shorter measurement times expected in next-generation synchrotron sources.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0012704

OSTI ID:: 1607717

Report Number(s):: BNL--213760-2020-JAAM

Journal Information:: Journal of Synchrotron Radiation (Online), Journal Name: Journal of Synchrotron Radiation (Online) Journal Issue: 2 Vol. 27; ISSN 1600-5775; ISSN JSYRES

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

References (19)

Evolutionary-Optimized Photonic Network Structure in White Beetle Wing Scales Wilts, Bodo D.; Sheng, Xiaoyuan; Holler, Mirko Advanced Materials, Vol. 30, Issue 19 https://doi.org/10.1002/adma.201702057	journal	June 2017
Evolution of a Rhythmic Lamination in the Organophosphatic Shells of Brachiopods Williams, Alwyn; Cusack, Maggie Journal of Structural Biology, Vol. 126, Issue 3 https://doi.org/10.1006/jsbi.1999.4117	journal	June 1999
Fourier shell correlation threshold criteria van Heel, Marin; Schatz, Michael Journal of Structural Biology, Vol. 151, Issue 3 https://doi.org/10.1016/j.jsb.2005.05.009	journal	September 2005
X-ray Microscopy Jacobsen, Chris Cambridge University Press https://doi.org/10.1017/9781139924542	book	January 2019
Mass Density and Water Content of Saturated Never-Dried Calcium Silicate Hydrates da Silva, Julio C.; Trtik, Pavel; Diaz, Ana Langmuir, Vol. 31, Issue 13 https://doi.org/10.1021/la504478j	journal	March 2015
Ptychographic X-ray computed tomography at the nanoscale Dierolf, Martin; Menzel, Andreas; Thibault, Pierre Nature, Vol. 467, Issue 7314 https://doi.org/10.1038/nature09419	journal	September 2010
Nanostructure surveys of macroscopic specimens by small-angle scattering tensor tomography Liebi, Marianne; Georgiadis, Marios; Menzel, Andreas Nature, Vol. 527, Issue 7578 https://doi.org/10.1038/nature16056	journal	November 2015
High-resolution non-destructive three-dimensional imaging of integrated circuits Holler, Mirko; Guizar-Sicairos, Manuel; Tsai, Esther H. R. Nature, Vol. 543, Issue 7645 https://doi.org/10.1038/nature21698	journal	March 2017
Three-dimensional magnetization structures revealed with X-ray vector nanotomography Donnelly, Claire; Guizar-Sicairos, Manuel; Scagnoli, Valerio Nature, Vol. 547, Issue 7663 https://doi.org/10.1038/nature23006	journal	July 2017
Three-Dimensional Imaging of Biological Tissue by Cryo X-Ray Ptychography Shahmoradian, S. H.; Tsai, E. H. R.; Diaz, A. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-05587-4	journal	July 2017
Multi-slice ptychographic tomography Li, Peng; Maiden, Andrew Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-20530-x	journal	February 2018
X-ray ptychographic computed tomography at 16 nm isotropic 3D resolution Holler, M.; Diaz, A.; Guizar-Sicairos, M. Scientific Reports, Vol. 4, Issue 1 https://doi.org/10.1038/srep03857	journal	January 2014
OMNY PIN—A versatile sample holder for tomographic measurements at room and cryogenic temperatures Holler, M.; Raabe, J.; Wepf, R. Review of Scientific Instruments, Vol. 88, Issue 11 https://doi.org/10.1063/1.4996092	journal	November 2017
OMNY—A tOMography Nano crYo stage Holler, M.; Raabe, J.; Diaz, A. Review of Scientific Instruments, Vol. 89, Issue 4 https://doi.org/10.1063/1.5020247	journal	April 2018
Material properties of brachiopod shell ultrastructure by nanoindentation Pérez-Huerta, Alberto; Cusack, Maggie; Zhu, Wenzhong Journal of The Royal Society Interface, Vol. 4, Issue 12 https://doi.org/10.1098/rsif.2006.0150	journal	August 2006
Challenges of microtome-based serial block-face scanning electron microscopy in neuroscience: CHALLENGES OF SBEM IN NEUROSCIENCE Wanner, A. A.; Kirschmann, M. A.; Genoud, C. Journal of Microscopy, Vol. 259, Issue 2 https://doi.org/10.1111/jmi.12244	journal	April 2015
Nanoscale Hard X-Ray Microscopy Methods for Materials Studies Holt, Martin; Harder, Ross; Winarski, Robert Annual Review of Materials Research, Vol. 43, Issue 1 https://doi.org/10.1146/annurev-matsci-071312-121654	journal	July 2013
LASER MICRODISSECTION OF PLANT TISSUE: What You See Is What You Get Nelson, Timothy; Tausta, S. Lori; Gandotra, Neeru Annual Review of Plant Biology, Vol. 57, Issue 1 https://doi.org/10.1146/annurev.arplant.56.032604.144138	journal	June 2006
Phase retrieval with transverse translation diversity: a nonlinear optimization approach Guizar-Sicairos, Manuel; Fienup, James R. Optics Express, Vol. 16, Issue 10 https://doi.org/10.1364/OE.16.007264	journal	January 2008

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Title: A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures

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