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Title: Temporospatial shifts in the human gut microbiome and metabolome after gastric bypass surgery

Abstract

Although the etiology of obesity is not well-understood, genetic, environmental, and microbiome elements are recognized as contributors to this rising pandemic. For morbid obesity, Roux-en-Y gastric bypass (RYGB) surgery alters the fecal microbiome, but data are sparse on temporal and spatial changes in the microbiome and metabolome. We characterized the structure and metabolism of the microbial communities in the gut lumen and on mucosal surfaces in morbidly obese individuals before and after RYGB surgery, and we compared our longitudinal cohort to a previously studied cross-sectional one. RYGB concurrently changed the gut microbiome and led to improvements of obesity comorbidities. Changes in the gut microbiome were reflected in the metabolome, including fermentation products and bile acids. The effects persisted 12 months post-surgery, and the microbiomes and metabolomes were similar to those found for the cross-sectional RYGB cohort. Thus, RYGB surgery had profound and persistent impacts on the intestinal microbiome and metabolome.

Authors:
 [1];  [2];  [3];  [3]; ORCiD logo [4]; ORCiD logo [3];  [4]; ORCiD logo [3];  [4]; ORCiD logo [4];  [2];  [1];  [1];  [1]
  1. Arizona State Univ., Tempe, AZ (United States)
  2. Mayo Clinic, Scottsdale, AZ (United States). Division of Gastroenterology
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
  4. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1607642
Report Number(s):
PNNL-SA-133750
Journal ID: ISSN 2055-5008
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
npj Biofilms and Microbiomes
Additional Journal Information:
Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 2055-5008
Publisher:
Springer Nature
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; gastric bypass; gut metabolome; bile acids; mucosa-associated microbiome; lumenassociated microbiome; fermentation

Citation Formats

Ilhan, Zehra E., DiBaise, John K., Dautel, Sydney E., Isern, Nancy G., Kim, Young-Mo, Hoyt, David W., Schepmoes, Athena A., Brewer, Heather M., Weitz, Karl K., Metz, Thomas O., Crowell, Michael, Kang, Dae Wook, Rittmann, Bruce E., and Krajmalnik-Brown, Rosa. Temporospatial shifts in the human gut microbiome and metabolome after gastric bypass surgery. United States: N. p., 2020. Web. doi:10.1038/s41522-020-0122-5.
Ilhan, Zehra E., DiBaise, John K., Dautel, Sydney E., Isern, Nancy G., Kim, Young-Mo, Hoyt, David W., Schepmoes, Athena A., Brewer, Heather M., Weitz, Karl K., Metz, Thomas O., Crowell, Michael, Kang, Dae Wook, Rittmann, Bruce E., & Krajmalnik-Brown, Rosa. Temporospatial shifts in the human gut microbiome and metabolome after gastric bypass surgery. United States. doi:https://doi.org/10.1038/s41522-020-0122-5
Ilhan, Zehra E., DiBaise, John K., Dautel, Sydney E., Isern, Nancy G., Kim, Young-Mo, Hoyt, David W., Schepmoes, Athena A., Brewer, Heather M., Weitz, Karl K., Metz, Thomas O., Crowell, Michael, Kang, Dae Wook, Rittmann, Bruce E., and Krajmalnik-Brown, Rosa. Fri . "Temporospatial shifts in the human gut microbiome and metabolome after gastric bypass surgery". United States. doi:https://doi.org/10.1038/s41522-020-0122-5. https://www.osti.gov/servlets/purl/1607642.
@article{osti_1607642,
title = {Temporospatial shifts in the human gut microbiome and metabolome after gastric bypass surgery},
author = {Ilhan, Zehra E. and DiBaise, John K. and Dautel, Sydney E. and Isern, Nancy G. and Kim, Young-Mo and Hoyt, David W. and Schepmoes, Athena A. and Brewer, Heather M. and Weitz, Karl K. and Metz, Thomas O. and Crowell, Michael and Kang, Dae Wook and Rittmann, Bruce E. and Krajmalnik-Brown, Rosa},
abstractNote = {Although the etiology of obesity is not well-understood, genetic, environmental, and microbiome elements are recognized as contributors to this rising pandemic. For morbid obesity, Roux-en-Y gastric bypass (RYGB) surgery alters the fecal microbiome, but data are sparse on temporal and spatial changes in the microbiome and metabolome. We characterized the structure and metabolism of the microbial communities in the gut lumen and on mucosal surfaces in morbidly obese individuals before and after RYGB surgery, and we compared our longitudinal cohort to a previously studied cross-sectional one. RYGB concurrently changed the gut microbiome and led to improvements of obesity comorbidities. Changes in the gut microbiome were reflected in the metabolome, including fermentation products and bile acids. The effects persisted 12 months post-surgery, and the microbiomes and metabolomes were similar to those found for the cross-sectional RYGB cohort. Thus, RYGB surgery had profound and persistent impacts on the intestinal microbiome and metabolome.},
doi = {10.1038/s41522-020-0122-5},
journal = {npj Biofilms and Microbiomes},
number = 1,
volume = 6,
place = {United States},
year = {2020},
month = {3}
}

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