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Title: Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity

Abstract

The Antarctic green alga Chlamydomonas sp. UWO 241 (UWO 241) is adapted to permanent low temperatures, hypersalinity, and extreme shade. One of the most striking phenotypes of UWO 241 is an altered PSI organization and constitutive PSI cyclic electron flow (CEF). To date, little attention has been paid to CEF during long-term stress acclimation, and the consequences of sustained CEF in UWO 241 are not known. In this study, we combined photobiology, proteomics, and metabolomics to understand the underlying role of sustained CEF in high-salinity stress acclimation. High salt-grown UWO 241 exhibited increased thylakoid proton motive flux and an increased capacity for nonphotochemical quenching. Under high salt, a significant proportion of the up-regulafted enzymes were associated with the Calvin-Benson-Bassham cycle, carbon storage metabolism, and protein translation. Two key enzymes of the shikimate pathway, 3-deoxy-d-arabinoheptulosonate 7-phosphate synthase and chorismate synthase, were also up-regulated, as well as indole-3-glycerol phosphate synthase, an enzyme involved in the biosynthesis of l-Trp and indole acetic acid. In addition, several compatible solutes (glycerol, Pro, and Suc) accumulated to high levels in high salt-grown UWO 241 cultures. We suggest that UWO 241 maintains constitutively high CEF through the associated PSI-cytochrome b6f supercomplex to support robust growth and strongmore » photosynthetic capacity under a constant growth regime of low temperatures and high salinity.« less

Authors:
ORCiD logo [1]; ORCiD logo [1];  [2];  [3];  [3];  [3]; ORCiD logo [4];  [5]; ORCiD logo [1]
  1. Department of Microbiology, Miami University, Oxford, Ohio 45056
  2. Department of Biology, University of Ottawa, Ottawa K1N 6N5, Ontario, Canada
  3. Donald Danforth Plant Science Center, St. Louis, Missouri 63132
  4. Department of Biology and Biotron Centre for Experimental Climate Change, University of Western Ontario, London N6A 3K7, Ontario, Canada
  5. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77840
Publication Date:
Research Org.:
Miami Univ., Oxford, OH (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)
OSTI Identifier:
1607621
Alternate Identifier(s):
OSTI ID: 1657213
Grant/Contract Number:  
SC0019138; SC0019464; 1637708
Resource Type:
Published Article
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Name: Plant Physiology (Bethesda) Journal Volume: 183 Journal Issue: 2; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
79 ASTRONOMY AND ASTROPHYSICS

Citation Formats

Kalra, Isha, Wang, Xin, Cvetkovska, Marina, Jeong, Jooyeon, McHargue, William, Zhang, Ru, Hüner, Norman, Yuan, Joshua S., and Morgan-Kiss, Rachael. Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity. United States: N. p., 2020. Web. doi:10.1104/pp.19.01280.
Kalra, Isha, Wang, Xin, Cvetkovska, Marina, Jeong, Jooyeon, McHargue, William, Zhang, Ru, Hüner, Norman, Yuan, Joshua S., & Morgan-Kiss, Rachael. Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity. United States. https://doi.org/10.1104/pp.19.01280
Kalra, Isha, Wang, Xin, Cvetkovska, Marina, Jeong, Jooyeon, McHargue, William, Zhang, Ru, Hüner, Norman, Yuan, Joshua S., and Morgan-Kiss, Rachael. Mon . "Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity". United States. https://doi.org/10.1104/pp.19.01280.
@article{osti_1607621,
title = {Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity},
author = {Kalra, Isha and Wang, Xin and Cvetkovska, Marina and Jeong, Jooyeon and McHargue, William and Zhang, Ru and Hüner, Norman and Yuan, Joshua S. and Morgan-Kiss, Rachael},
abstractNote = {The Antarctic green alga Chlamydomonas sp. UWO 241 (UWO 241) is adapted to permanent low temperatures, hypersalinity, and extreme shade. One of the most striking phenotypes of UWO 241 is an altered PSI organization and constitutive PSI cyclic electron flow (CEF). To date, little attention has been paid to CEF during long-term stress acclimation, and the consequences of sustained CEF in UWO 241 are not known. In this study, we combined photobiology, proteomics, and metabolomics to understand the underlying role of sustained CEF in high-salinity stress acclimation. High salt-grown UWO 241 exhibited increased thylakoid proton motive flux and an increased capacity for nonphotochemical quenching. Under high salt, a significant proportion of the up-regulafted enzymes were associated with the Calvin-Benson-Bassham cycle, carbon storage metabolism, and protein translation. Two key enzymes of the shikimate pathway, 3-deoxy-d-arabinoheptulosonate 7-phosphate synthase and chorismate synthase, were also up-regulated, as well as indole-3-glycerol phosphate synthase, an enzyme involved in the biosynthesis of l-Trp and indole acetic acid. In addition, several compatible solutes (glycerol, Pro, and Suc) accumulated to high levels in high salt-grown UWO 241 cultures. We suggest that UWO 241 maintains constitutively high CEF through the associated PSI-cytochrome b6f supercomplex to support robust growth and strong photosynthetic capacity under a constant growth regime of low temperatures and high salinity.},
doi = {10.1104/pp.19.01280},
journal = {Plant Physiology (Bethesda)},
number = 2,
volume = 183,
place = {United States},
year = {2020},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1104/pp.19.01280

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