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Title: A DNA G-quadruplex/i-motif hybrid

Abstract

Abstract DNA can form many structures beyond the canonical Watson–Crick double helix. It is now clear that noncanonical structures are present in genomic DNA and have biological functions. G-rich G-quadruplexes and C-rich i-motifs are the most well-characterized noncanonical DNA motifs that have been detected in vivo with either proscribed or postulated biological roles. Because of their independent sequence requirements, these structures have largely been considered distinct types of quadruplexes. Here, we describe the crystal structure of the DNA oligonucleotide, d(CCAGGCTGCAA), that self-associates to form a quadruplex structure containing two central antiparallel G-tetrads and six i-motif C–C+ base pairs. Solution studies suggest a robust structural motif capable of assembling as a tetramer of individual strands or as a dimer when composed of tandem repeats. This hybrid structure highlights the growing structural diversity of DNA and suggests that biological systems may harbor many functionally important non-duplex structures.

Authors:
 [1];  [1];  [1]
+ Show Author Affiliations
  1. Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742, USA
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE Office of Science (SC); National Institutes of Health (NIH)
OSTI Identifier:
1607551
Alternate Identifier(s):
OSTI ID: 1578242
Grant/Contract Number:  
AC02-05CH11231; P30 GM124165; S10RR25528; S10RR02897
Resource Type:
Published Article
Journal Name:
Nucleic Acids Research
Additional Journal Information:
Journal Name: Nucleic Acids Research; Journal ID: ISSN 0305-1048
Publisher:
Oxford University Press
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Structural biology

Citation Formats

Chu, Betty, Zhang, Daoning, and Paukstelis, Paul J. A DNA G-quadruplex/i-motif hybrid. United Kingdom: N. p., 2019. Web. doi:10.1093/nar/gkz1008.
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Chu, Betty, Zhang, Daoning, & Paukstelis, Paul J. A DNA G-quadruplex/i-motif hybrid. United Kingdom. https://doi.org/10.1093/nar/gkz1008
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Chu, Betty, Zhang, Daoning, and Paukstelis, Paul J. Thu . "A DNA G-quadruplex/i-motif hybrid". United Kingdom. https://doi.org/10.1093/nar/gkz1008.
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@article{osti_1607551,
title = {A DNA G-quadruplex/i-motif hybrid},
author = {Chu, Betty and Zhang, Daoning and Paukstelis, Paul J.},
abstractNote = {Abstract DNA can form many structures beyond the canonical Watson–Crick double helix. It is now clear that noncanonical structures are present in genomic DNA and have biological functions. G-rich G-quadruplexes and C-rich i-motifs are the most well-characterized noncanonical DNA motifs that have been detected in vivo with either proscribed or postulated biological roles. Because of their independent sequence requirements, these structures have largely been considered distinct types of quadruplexes. Here, we describe the crystal structure of the DNA oligonucleotide, d(CCAGGCTGCAA), that self-associates to form a quadruplex structure containing two central antiparallel G-tetrads and six i-motif C–C+ base pairs. Solution studies suggest a robust structural motif capable of assembling as a tetramer of individual strands or as a dimer when composed of tandem repeats. This hybrid structure highlights the growing structural diversity of DNA and suggests that biological systems may harbor many functionally important non-duplex structures.},
doi = {10.1093/nar/gkz1008},
journal = {Nucleic Acids Research},
number = ,
volume = ,
place = {United Kingdom},
year = {2019},
month = {11}
}
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https://doi.org/10.1093/nar/gkz1008
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