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Title: Single-Cell Genomics and the Oral Microbiome

Abstract

The human oral cavity is one of the first environments where microbes have been discovered and studied since the dawn of microbiology. Nevertheless, approximately 200 types of bacteria from the oral microbiota have remained uncultured in the laboratory. Some are associated with a healthy oral microbial community, while others are linked to oral diseases, from dental caries to gum disease. Single-cell genomics has enabled inferences on the physiology, virulence, and evolution of such uncultured microorganisms and has further enabled isolation and cultivation of several novel oral bacteria, including the discovery of novel interspecies interactions. This review summarizes some of the more recent advances in this field, which is rapidly moving toward physiologic characterization of single cells and ultimately cultivation of the yet uncultured. A combination of traditional microbiological approaches with genomic-based physiologic predictions and isolation strategies may lead to the oral microbiome being the first complex microbial community to have all its members cultivable in the laboratory. We conclude studying the biology of the individual microbes when in association with other members of the community, in controlled laboratory conditions and in vivo, should lead to a better understanding of oral dysbiosis and its prevention and reversion.

Authors:
ORCiD logo [1];  [2]; ORCiD logo [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Vanderbilt Univ., Nashville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; National Institute of Dental and Craniofacial Research (NIDCR); National Institutes of Health (NIH)
OSTI Identifier:
1606754
Grant/Contract Number:  
AC05-00OR22725; R01DE024463
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Dental Research
Additional Journal Information:
Journal Volume: 99; Journal Issue: 6; Journal ID: ISSN 0022-0345
Publisher:
SAGE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bacteria; bioinformatics; microbiology; biofilms; microbial ecology; dysbiosis

Citation Formats

Balachandran, Manasi, Cross, Karissa L., and Podar, Mircea. Single-Cell Genomics and the Oral Microbiome. United States: N. p., 2020. Web. doi:10.1177/0022034520907380.
Balachandran, Manasi, Cross, Karissa L., & Podar, Mircea. Single-Cell Genomics and the Oral Microbiome. United States. doi:https://doi.org/10.1177/0022034520907380
Balachandran, Manasi, Cross, Karissa L., and Podar, Mircea. Mon . "Single-Cell Genomics and the Oral Microbiome". United States. doi:https://doi.org/10.1177/0022034520907380. https://www.osti.gov/servlets/purl/1606754.
@article{osti_1606754,
title = {Single-Cell Genomics and the Oral Microbiome},
author = {Balachandran, Manasi and Cross, Karissa L. and Podar, Mircea},
abstractNote = {The human oral cavity is one of the first environments where microbes have been discovered and studied since the dawn of microbiology. Nevertheless, approximately 200 types of bacteria from the oral microbiota have remained uncultured in the laboratory. Some are associated with a healthy oral microbial community, while others are linked to oral diseases, from dental caries to gum disease. Single-cell genomics has enabled inferences on the physiology, virulence, and evolution of such uncultured microorganisms and has further enabled isolation and cultivation of several novel oral bacteria, including the discovery of novel interspecies interactions. This review summarizes some of the more recent advances in this field, which is rapidly moving toward physiologic characterization of single cells and ultimately cultivation of the yet uncultured. A combination of traditional microbiological approaches with genomic-based physiologic predictions and isolation strategies may lead to the oral microbiome being the first complex microbial community to have all its members cultivable in the laboratory. We conclude studying the biology of the individual microbes when in association with other members of the community, in controlled laboratory conditions and in vivo, should lead to a better understanding of oral dysbiosis and its prevention and reversion.},
doi = {10.1177/0022034520907380},
journal = {Journal of Dental Research},
number = 6,
volume = 99,
place = {United States},
year = {2020},
month = {2}
}

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