An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems

Zhang, Jin; Chen, Jun; Wang, Lijuan; Zhao, Shutang; Wang, Weina; Li, Jianbo; Liu, Bobin; Qi, Xingyun; Zheng, Huanquan; Lu, Mengzhu

doi:10.1007/s00299-019-02497-9

Title: An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems

Journal Article · 10 December 2019 · Plant Cell Reports

DOI: https://doi.org/10.1007/s00299-019-02497-9 · OSTI ID:1606648

^[1]; Chen, Jun ^[2]; Wang, Lijuan ^[3]; Zhao, Shutang ^[3]; Wang, Weina ^[4]; Li, Jianbo ^[3]; Liu, Bobin ^[3]; Qi, Xingyun ^[4]; Zheng, Huanquan ^[4]; Lu, Mengzhu ^[5]

Chinese Academy of Forestry, Beijing (China); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Chinese Academy of Forestry, Beijing (China); McGill Univ., Montreal, QC (Canada)
Chinese Academy of Forestry, Beijing (China)
McGill Univ., Montreal, QC (Canada)
Chinese Academy of Forestry, Beijing (China); Zhejiang A&F Univ. (China)

TRAPP complexes, composed of multimeric subunits, are guanine–nucleotide exchange factors for certain Rab GTPases and are believed to be involved in the regulation of membrane trafficking, but the cases in Arabidopsis are largely unknown. Trs33, recently proposed to be a component of TRAPP IV, is non-essential in yeast cells. A single copy of Trs33 gene, AtTrs33, was identified in Arabidopsis. GUS activity assay indicated that AtTrs33 was ubiquitously expressed. Based on a T-DNA insertion line, we found that loss-of-function of AtTrs33 is lethal for apical growth. Knock-down or knock-in of AtTrs33 affects apical meristematic growth and fertility, which indicates that AtTrs33 plays an important role in keeping apical meristematic activity and dominance in Arabidopsis. Analysis of auxin responses and PIN1/2 localization indicate that impaired apical meristematic activity and dominance were caused by altered auxin responses through non-polarized PIN1 localization. The present study reported that AtTrs33 plays an essential role in Arabidopsis cell growth and organization, which is different with its homologue in yeast. These findings provide new insights into the functional divergence of TRAPP subunits.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: Chinese Academy of Forestry; McGill Univ.; The National Science and Engineering Research Council of Canada; USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1606648

Journal Information:: Plant Cell Reports, Journal Name: Plant Cell Reports Journal Issue: 3 Vol. 39; ISSN 0721-7714

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Arabidopsis
PIN1/2
TRAPP
Trs33
apical meristem
auxin
subcellular localization

Title: An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems

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References (47)

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