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Title: An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems

Abstract

TRAPP complexes, composed of multimeric subunits, are guanine–nucleotide exchange factors for certain Rab GTPases and are believed to be involved in the regulation of membrane trafficking, but the cases in Arabidopsis are largely unknown. Trs33, recently proposed to be a component of TRAPP IV, is non-essential in yeast cells. A single copy of Trs33 gene, AtTrs33, was identified in Arabidopsis. GUS activity assay indicated that AtTrs33 was ubiquitously expressed. Based on a T-DNA insertion line, we found that loss-of-function of AtTrs33 is lethal for apical growth. Knock-down or knock-in of AtTrs33 affects apical meristematic growth and fertility, which indicates that AtTrs33 plays an important role in keeping apical meristematic activity and dominance in Arabidopsis. Analysis of auxin responses and PIN1/2 localization indicate that impaired apical meristematic activity and dominance were caused by altered auxin responses through non-polarized PIN1 localization. The present study reported that AtTrs33 plays an essential role in Arabidopsis cell growth and organization, which is different with its homologue in yeast. These findings provide new insights into the functional divergence of TRAPP subunits.

Authors:
ORCiD logo [1];  [2];  [3];  [3];  [4];  [3];  [3];  [4];  [4];  [5]
  1. Chinese Academy of Forestry, Beijing (China); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Chinese Academy of Forestry, Beijing (China); McGill Univ., Montreal, QC (Canada)
  3. Chinese Academy of Forestry, Beijing (China)
  4. McGill Univ., Montreal, QC (Canada)
  5. Chinese Academy of Forestry, Beijing (China); Zhejiang A&F Univ. (China)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; The National Science and Engineering Research Council of Canada; Chinese Academy of Forestry; McGill Univ.
OSTI Identifier:
1606648
Grant/Contract Number:  
AC05-00OR22725; CAFYBB2011001
Resource Type:
Accepted Manuscript
Journal Name:
Plant Cell Reports
Additional Journal Information:
Journal Volume: 39; Journal Issue: 3; Journal ID: ISSN 0721-7714
Publisher:
Springer Nature
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Arabidopsis; Trs33; TRAPP; apical meristem; auxin; PIN1/2; subcellular localization

Citation Formats

Zhang, Jin, Chen, Jun, Wang, Lijuan, Zhao, Shutang, Wang, Weina, Li, Jianbo, Liu, Bobin, Qi, Xingyun, Zheng, Huanquan, and Lu, Mengzhu. An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems. United States: N. p., 2019. Web. https://doi.org/10.1007/s00299-019-02497-9.
Zhang, Jin, Chen, Jun, Wang, Lijuan, Zhao, Shutang, Wang, Weina, Li, Jianbo, Liu, Bobin, Qi, Xingyun, Zheng, Huanquan, & Lu, Mengzhu. An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems. United States. https://doi.org/10.1007/s00299-019-02497-9
Zhang, Jin, Chen, Jun, Wang, Lijuan, Zhao, Shutang, Wang, Weina, Li, Jianbo, Liu, Bobin, Qi, Xingyun, Zheng, Huanquan, and Lu, Mengzhu. Wed . "An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems". United States. https://doi.org/10.1007/s00299-019-02497-9. https://www.osti.gov/servlets/purl/1606648.
@article{osti_1606648,
title = {An essential role for Arabidopsis Trs33 in cell growth and organization in plant apical meristems},
author = {Zhang, Jin and Chen, Jun and Wang, Lijuan and Zhao, Shutang and Wang, Weina and Li, Jianbo and Liu, Bobin and Qi, Xingyun and Zheng, Huanquan and Lu, Mengzhu},
abstractNote = {TRAPP complexes, composed of multimeric subunits, are guanine–nucleotide exchange factors for certain Rab GTPases and are believed to be involved in the regulation of membrane trafficking, but the cases in Arabidopsis are largely unknown. Trs33, recently proposed to be a component of TRAPP IV, is non-essential in yeast cells. A single copy of Trs33 gene, AtTrs33, was identified in Arabidopsis. GUS activity assay indicated that AtTrs33 was ubiquitously expressed. Based on a T-DNA insertion line, we found that loss-of-function of AtTrs33 is lethal for apical growth. Knock-down or knock-in of AtTrs33 affects apical meristematic growth and fertility, which indicates that AtTrs33 plays an important role in keeping apical meristematic activity and dominance in Arabidopsis. Analysis of auxin responses and PIN1/2 localization indicate that impaired apical meristematic activity and dominance were caused by altered auxin responses through non-polarized PIN1 localization. The present study reported that AtTrs33 plays an essential role in Arabidopsis cell growth and organization, which is different with its homologue in yeast. These findings provide new insights into the functional divergence of TRAPP subunits.},
doi = {10.1007/s00299-019-02497-9},
journal = {Plant Cell Reports},
number = 3,
volume = 39,
place = {United States},
year = {2019},
month = {12}
}

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