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Title: The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar

Abstract

WUSCHEL-RELATED HOMEOBOX (WOX) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the WOX family, WUSCHEL (WUS) is characterized for its role in maintaining stem cell in meristem. In this study, we investigated the function of Populus tomentosa WUSCHELa (PtoWUSa) in adventitious roots (ARs) in poplar. Expression profile analysis showed that PtoWUSa was not only expressed in shoot apical meristem and stem, but also expressed in ARs. Ectopic expression of PtoWUSa in Arabidopsis resulted in shortened primary root, as well as agravitropism and multiple branches. Overexpression of PtoWUSa in poplar increased the number of ARs but decreased their length. Moreover, the AR tip and lateral root tip became larger and swollen. In addition, the expression of auxin transporter genes PIN-FORMED were downregulated in ARs of transgenic plant. Taken together, these results suggest that PtoWUSa could be involved in AR development in poplar through regulating the polar auxin transport in ARs.

Authors:
 [1];  [1];  [1]; ORCiD logo [2];  [1];  [1]; ORCiD logo [1];  [3]
  1. Chinese Academy of Forestry, Beijing (China)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Zhejiang Agriculture & Forestry Univ., Hangzhou (China)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; National Key Program on Transgenic Research; National Natural Science Foundation of China (NNSFC); Fundamental Research Funds of Chinese Academy of Forestry
OSTI Identifier:
1606647
Alternate Identifier(s):
OSTI ID: 1761674
Grant/Contract Number:  
AC05-00OR22725; 2018ZX08020002; 31800569; CAFYBB2019ZY003
Resource Type:
Accepted Manuscript
Journal Name:
Genes
Additional Journal Information:
Journal Volume: 11; Journal Issue: 2; Journal ID: ISSN 2073-4425
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; adventitious roots; auxin; poplar; PtoWUSa; transgenic plant

Citation Formats

Li, Jianbo, Jia, Huixia, Sun, Pei, Zhang, Jin, Xia, Yongxiu, Hu, Jianjun, Wang, Lijuan, and Lu, Mengzhu. The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar. United States: N. p., 2020. Web. https://doi.org/10.3390/genes11020176.
Li, Jianbo, Jia, Huixia, Sun, Pei, Zhang, Jin, Xia, Yongxiu, Hu, Jianjun, Wang, Lijuan, & Lu, Mengzhu. The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar. United States. https://doi.org/10.3390/genes11020176
Li, Jianbo, Jia, Huixia, Sun, Pei, Zhang, Jin, Xia, Yongxiu, Hu, Jianjun, Wang, Lijuan, and Lu, Mengzhu. Thu . "The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar". United States. https://doi.org/10.3390/genes11020176. https://www.osti.gov/servlets/purl/1606647.
@article{osti_1606647,
title = {The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar},
author = {Li, Jianbo and Jia, Huixia and Sun, Pei and Zhang, Jin and Xia, Yongxiu and Hu, Jianjun and Wang, Lijuan and Lu, Mengzhu},
abstractNote = {WUSCHEL-RELATED HOMEOBOX (WOX) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the WOX family, WUSCHEL (WUS) is characterized for its role in maintaining stem cell in meristem. In this study, we investigated the function of Populus tomentosa WUSCHELa (PtoWUSa) in adventitious roots (ARs) in poplar. Expression profile analysis showed that PtoWUSa was not only expressed in shoot apical meristem and stem, but also expressed in ARs. Ectopic expression of PtoWUSa in Arabidopsis resulted in shortened primary root, as well as agravitropism and multiple branches. Overexpression of PtoWUSa in poplar increased the number of ARs but decreased their length. Moreover, the AR tip and lateral root tip became larger and swollen. In addition, the expression of auxin transporter genes PIN-FORMED were downregulated in ARs of transgenic plant. Taken together, these results suggest that PtoWUSa could be involved in AR development in poplar through regulating the polar auxin transport in ARs.},
doi = {10.3390/genes11020176},
journal = {Genes},
number = 2,
volume = 11,
place = {United States},
year = {2020},
month = {2}
}

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