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Title: Building customizable auto-luminescent luciferase-based reporters in plants

Abstract

Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP) in planta using a composable toolbox of parts. We demonstrate that the FBP can create luminescence across various tissues in a broad range of plants without external substrate addition. We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. A low-cost imaging platform for gene expression profiling is also described. These experiments lay the groundwork for future construction of programmable auto-luminescent plant traits, such as light driven plant-pollinator interactions or light emitting plant-based sensors.

Authors:
ORCiD logo [1]; ORCiD logo [1];  [1];  [2];  [1];  [1];  [1];  [1];  [2]; ORCiD logo [1]
  1. Department Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States, Center for Precision Plant Genomics, University of Minnesota, St. Paul, United States
  2. Department of Biology, University of Washington, Seattle, United States
Publication Date:
Research Org.:
Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of Minnesota, Minneapolis, MN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
OSTI Identifier:
1606276
Alternate Identifier(s):
OSTI ID: 1606277; OSTI ID: 1633413; OSTI ID: 1659403
Grant/Contract Number:  
SC0018277
Resource Type:
Published Article
Journal Name:
eLife
Additional Journal Information:
Journal Name: eLife Journal Volume: 9; Journal ID: ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Khakhar, Arjun, Starker, Colby G., Chamness, James C., Lee, Nayoung, Stokke, Sydney, Wang, Cecily, Swanson, Ryan, Rizvi, Furva, Imaizumi, Takato, and Voytas, Daniel F. Building customizable auto-luminescent luciferase-based reporters in plants. United States: N. p., 2020. Web. doi:10.7554/eLife.52786.
Khakhar, Arjun, Starker, Colby G., Chamness, James C., Lee, Nayoung, Stokke, Sydney, Wang, Cecily, Swanson, Ryan, Rizvi, Furva, Imaizumi, Takato, & Voytas, Daniel F. Building customizable auto-luminescent luciferase-based reporters in plants. United States. doi:https://doi.org/10.7554/eLife.52786
Khakhar, Arjun, Starker, Colby G., Chamness, James C., Lee, Nayoung, Stokke, Sydney, Wang, Cecily, Swanson, Ryan, Rizvi, Furva, Imaizumi, Takato, and Voytas, Daniel F. Wed . "Building customizable auto-luminescent luciferase-based reporters in plants". United States. doi:https://doi.org/10.7554/eLife.52786.
@article{osti_1606276,
title = {Building customizable auto-luminescent luciferase-based reporters in plants},
author = {Khakhar, Arjun and Starker, Colby G. and Chamness, James C. and Lee, Nayoung and Stokke, Sydney and Wang, Cecily and Swanson, Ryan and Rizvi, Furva and Imaizumi, Takato and Voytas, Daniel F.},
abstractNote = {Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP) in planta using a composable toolbox of parts. We demonstrate that the FBP can create luminescence across various tissues in a broad range of plants without external substrate addition. We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. A low-cost imaging platform for gene expression profiling is also described. These experiments lay the groundwork for future construction of programmable auto-luminescent plant traits, such as light driven plant-pollinator interactions or light emitting plant-based sensors.},
doi = {10.7554/eLife.52786},
journal = {eLife},
number = ,
volume = 9,
place = {United States},
year = {2020},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: https://doi.org/10.7554/eLife.52786

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Cited by: 1 work
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