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Title: Interprotein electron transfer biohybrid system for photocatalytic H2 production

Journal Article · · Photosynthesis Research
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  1. Argonne National Lab. (ANL), Argonne, IL (United States)
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Around the world there is a large research investment in developing solar fuel systems as clean and sustainable sources of energy. The fundamental mechanisms of natural photosynthesis can provide a source of inspiration for these studies. Photosynthetic reaction center (RC) proteins capture and convert light energy into chemical energy that is ultimately used to drive oxygenic water-splitting and carbon fixation. For the light energy to be used, the RC communicates with other donor/acceptor components via a sophisticated electron transfer scheme that includes electron transfer reactions between soluble and membrane bound proteins. Herein, we reengineer an inherent interprotein electron transfer pathway in a natural photosynthetic system to make it photocatalytic for aqueous H2 production. The native electron shuttle protein ferredoxin (Fd) is used as a scaffold for binding of a ruthenium photosensitizer and H2 catalytic function is imparted to its partner protein, ferredoxin-NADP+-reductase (FNR), by attachment of cobaloxime molecules. We find that this 2-protein biohybrid system produces H2 in aqueous solutions via light-induced interprotein electron transfer reactions (TON > 2500 H2/FNR), providing insight about using native protein-protein interactions as a method for fuel generation.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1606243
Journal Information:
Photosynthesis Research, Journal Name: Photosynthesis Research Journal Issue: 2 Vol. 143; ISSN 0166-8595
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English

References (44)

Interfacing Formate Dehydrogenase with Metal Oxides for the Reversible Electrocatalysis and Solar‐Driven Reduction of Carbon Dioxide journal February 2019
Interaction and electron transfer between ferredoxin–NADP+ oxidoreductase and its partners: structural, functional, and physiological implications journal March 2017
Association of Ferredoxin:NADP+ oxidoreductase with the photosynthetic apparatus modulates electron transfer in Chlamydomonas reinhardtii journal June 2017
Structure–function relationships in Anabaena ferredoxin/ferredoxin:NADP+ reductase electron transfer: insights from site-directed mutagenesis, transient absorption spectroscopy and X-ray crystallography journal April 2002
Mechanism of Coenzyme Recognition and Binding Revealed by Crystal Structure Analysis of Ferredoxin–NADP+ Reductase Complexed with NADP+ journal June 2002
Rational redesign of the ferredoxin-NADP+-oxido-reductase/ferredoxin-interaction for photosynthesis-dependent H2-production journal April 2018
Light-driven hydrogen production from Photosystem I-catalyst hybrids journal April 2015
Synechocystis ferredoxin/ferredoxin-NADP + -reductase/NADP + complex: Structural model obtained by NMR-restrained docking journal August 2005
EasySpin, a comprehensive software package for spectral simulation and analysis in EPR journal January 2006
Modular Homogeneous Chromophore–Catalyst Assemblies journal April 2016
Molecular Cobalt Catalysts for H 2 Generation with Redox Activity and Proton Relays in the Second Coordination Sphere journal December 2018
Charge Separation Related to Photocatalytic H 2 Production from a Ru–Apoflavodoxin–Ni Biohybrid journal December 2016
Wiring Photosystem I for Direct Solar Hydrogen Production journal January 2010
Role of Arg100 and Arg264 from Anabaena PCC 7119 Ferredoxin−NADP + Reductase for Optimal NADP + Binding and Electron Transfer † journal December 1998
Formation of thin polymeric films by electropolymerization. Reduction of metal complexes containing bromomethyl-substituted derivatives of 2,2'-bipyridine journal July 1991
Characterization of the structure, properties, and reactivity of a cobalt(II) macrocyclic complex journal November 1986
Chemical Approaches to Artificial Photosynthesis. 2 journal October 2005
Interfacial photoreactions at the photosynthetic membrane interface: an upper limit for the number of platinum atoms required to form a hydrogen-evolving platinum metal catalyst journal August 1988
Unimolecular and bimolecular homolytic reactions of organochromium and organocobalt complexes. Kinetics and equilibria journal September 1984
Nature-Driven Photochemistry for Catalytic Solar Hydrogen Production: A Photosystem I–Transition Metal Catalyst Hybrid journal October 2011
Protein Delivery of a Ni Catalyst to Photosystem I for Light-Driven Hydrogen Production journal August 2013
Photosystem I/Molecular Wire/Metal Nanoparticle Bioconjugates for the Photocatalytic Production of H 2 journal May 2008
Efficient and Clean Photoreduction of CO 2 to CO by Enzyme-Modified TiO 2 Nanoparticles Using Visible Light journal February 2010
Binuclear Ruthenium−Manganese Complexes as Simple Artificial Models for Photosystem II in Green Plants journal July 1997
Probing Local Dynamics of the Photosynthetic Bacterial Reaction Center with a Cysteine Specific Spin Label journal June 2003
The Hydrogen Catalyst Cobaloxime: A Multifrequency EPR and DFT Study of Cobaloxime’s Electronic Structure journal February 2012
Photocatalytic Hydrogen Production from Noncovalent Biohybrid Photosystem I/Pt Nanoparticle Complexes journal January 2011
Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP+ reductase journal February 2001
Self-organized photosynthetic nanoparticle for cell-free hydrogen production journal November 2009
Aqueous light driven hydrogen production by a Ru–ferredoxin–Co biohybrid journal January 2015
Ru–protein–Co biohybrids designed for solar hydrogen production: understanding electron transfer pathways related to photocatalytic function journal January 2016
Z-scheme solar water splitting via self-assembly of photosystem I-catalyst hybrids in thylakoid membranes journal January 2018
Midpoint redox potentials of plant and algal ferredoxins journal November 1977
Role of critical charged residues in reduction potential modulation of ferredoxin-NADP + reductase: Differential stabilization of FAD redox forms journal May 2002
Powering the planet: Chemical challenges in solar energy utilization journal October 2006
Solar hydrogen-producing bionanodevice outperforms natural photosynthesis journal December 2011
Influence of pH and ionic strength on electrostatic properties of ferredoxin, FNR, and hydrogenase and the rate constants of their interaction journal October 2016
Crystallographic studies of the interaction between the ferredoxin-NADP + reductase and ferredoxin from the cyanobacterium Anabaena : looking for the elusive ferredoxin molecule journal November 2000
Structure of the Mutant E92K of [2Fe–2S] Ferredoxin I from Spinacia oleracea at 1.7 Å Resolution journal November 1998
Analysis of the Oxidation-Reduction Potentials of Recombinant Ferredoxin-NADP+ Reductase from Spinach Chloroplasts journal August 1996
Structural and mechanistic aspects of flavoproteins: photosynthetic electron transfer from photosystem I to NADP + journal August 2009
Toward Cost-Effective Solar Energy Use journal February 2007
Light-driven dinitrogen reduction catalyzed by a CdS:nitrogenase MoFe protein biohybrid journal April 2016
Light-driven Hydrogen Production by a Hybrid Complex of a [NiFe]-Hydrogenase and the Cyanobacterial Photosystem I journal January 2006

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59 BASIC BIOLOGICAL SCIENCES
biohybrid
ferredoxin
ferredoxin-NADP+-reductase
photosynthetic electron transfer
solar fuel
solar hydrogen production