DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor

Journal Article · · Methods in Enzymology

IMAGINE is a high intensity, quasi-Laue neutron crystallography beamline developed at the 85 MW High Flux Isotope Reactor (HFIR) at Oak Ridge National Laboratory (ORNL). This state-of-the-art facility for neutron-diffraction enables neutron protein structures to be determined at or near atomic resolutions from crystals with volumes of < 1 mm3 and unit cell edges of < 150 Å. The beamline features include elliptical focusing mirrors that deliver neutrons into a 2.0 × 3.2 mm2 focal spot at the sample position, and variable short and long wavelength cutoff optics that provide automated exchange between multiple wavelength configurations. The beamline is equipped with a single-axis goniometer, neutron-sensitive cylindrical image plate detector and room temperature and cryogenic sample environments. This article describes the beamline components, the diffractometer and the data collection and data analysis protocols that are used, and outlines the protein deuteration, crystallization and conventional crystallography capabilities that are available to users at ORNL's neutron facilities. We also present examples of the scientific questions being addressed at this beamline and highlight important findings in enzyme chemistry that have been made possible by IMAGINE.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1606037
Journal Information:
Methods in Enzymology, Journal Name: Methods in Enzymology Journal Issue: 1 Vol. 634; ISSN 0076-6879
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English

References (36)

The IMAGINE instrument: first neutron protein structure and new capabilities for neutron macromolecular crystallography journal September 2013
The structure of concanavalin A and its bound solvent determined with small-molecule accuracy at 0.94 [Aring ]resolution journal January 1997
Oxygen Activation at the Active Site of a Fungal Lytic Polysaccharide Monooxygenase journal December 2016
The 15-K neutron structure of saccharide-free concanavalin A journal November 2004
γ-Phosphate Protonation and pH-dependent Unfolding of the Ras·GTP·Mg 2+ Complex : A VIBRATIONAL SPECTROSCOPY STUDY journal December 2000
LAUEGEN version 6.0 and INTLDM journal June 1998
The CG1 instrument development test station at the high flux isotope reactor
  • Crow, Lowell; Robertson, Lee; Bilheux, Hassina
  • Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 634, Issue 1 https://doi.org/10.1016/j.nima.2010.06.213
journal April 2011
Crystallization of a fungal lytic polysaccharide monooxygenase expressed from glycoengineered Pichia pastoris for X-ray and neutron diffraction journal January 2017
Features and development of Coot journal March 2010
LAUEGEN , an X-windows-based program for the processing of Laue diffraction data journal April 1995
Neutron cryo-crystallography captures the protonation state of ferryl heme in a peroxidase journal July 2014
The Bio-SANS instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory journal June 2014
High-temperature and high-resolution crystallography of thermostable copper nitrite reductase journal January 2015
Neutron protein crystallography at ultra-low (<15 K) temperatures journal June 2012
Temperature-dependent macromolecular X-ray crystallography journal March 2010
The recording and analysis of synchrotron X-radiation Laue diffraction photographs journal October 1989
Bio-SANS—A dedicated facility for neutron structural biology at Oak Ridge National Laboratory journal November 2006
Accessing protein conformational ensembles using room-temperature X-ray crystallography journal September 2011
Overview of the CCP 4 suite and current developments journal March 2011
The Phenix software for automated determination of macromolecular structures journal September 2011
Neutron Laue diffraction does it faster journal November 1997
An imaging plate neutron detector
  • Niimura, Nobuo; Karasawa, Yuuko; Tanaka, Inchiro
  • Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 349, Issue 2-3 https://doi.org/10.1016/0168-9002(94)91220-3
journal October 1994
Neutron Crystal Structure of RAS GTPase Puts in Question the Protonation State of the GTP γ-Phosphate journal October 2015
LSCALE – the new normalization, scaling and absorption correction program in the Daresbury Laue software suite journal June 1999
IMAGINE: neutrons reveal enzyme chemistry journal July 2018
The 40 m general purpose small-angle neutron scattering instrument at Oak Ridge National Laboratory journal August 2012
Generalized X-ray and neutron crystallographic analysis: more accurate and complete structures for biological macromolecules journal May 2009
Coot model-building tools for molecular graphics journal November 2004
Effects of temperature on protein structure and dynamics: x-ray crystallographic studies of the protein ribonuclease-A at nine different temperatures from 98 to 320K journal March 1992
Joint X-ray and neutron refinement with phenix.refine journal October 2010
Neutron protein crystallography: A complementary tool for locating hydrogens in proteins journal July 2016
CrystalPlan : an experiment-planning tool for crystallography journal March 2011
Neutron Laue macromolecular crystallography journal August 2006
Titration of ionizable groups in proteins using multiple neutron data sets from a single crystal: application to the small GTPase Ras journal January 2019
Neutron crystallographic studies of T4 lysozyme at cryogenic temperature: Neutron Crystallographic Studies of T4 Lysozyme journal September 2017
Rapid visualization of hydrogen positions in protein neutron crystallographic structures journal December 2011

Similar Records

IMAGINE: first neutron protein structure and new capabilities for neutron macromolecular crystallography
Journal Article · 2013 · Acta Crystallographica. Section D: Biological Crystallography · OSTI ID:1133535

Instrument development continues in Oak Ridge
Journal Article · 2012 · Notiziario Neutroni E Luce di Sincrotrone · OSTI ID:1032818

Protein crystallography beamline BL2S1 at the Aichi synchrotron
Journal Article · 2017 · Journal of Synchrotron Radiation (Online) · OSTI ID:1338972