IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor

Meilleur, Flora; Kovalevsky, Andrii Y.; Myles, Dean A A

doi:10.1016/bs.mie.2019.11.016

Title: IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor

Journal Article · 31 January 2020 · Methods in Enzymology

DOI: https://doi.org/10.1016/bs.mie.2019.11.016 · OSTI ID:1606037

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Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

IMAGINE is a high intensity, quasi-Laue neutron crystallography beamline developed at the 85 MW High Flux Isotope Reactor (HFIR) at Oak Ridge National Laboratory (ORNL). This state-of-the-art facility for neutron-diffraction enables neutron protein structures to be determined at or near atomic resolutions from crystals with volumes of < 1 mm³ and unit cell edges of < 150 Å. The beamline features include elliptical focusing mirrors that deliver neutrons into a 2.0 × 3.2 mm² focal spot at the sample position, and variable short and long wavelength cutoff optics that provide automated exchange between multiple wavelength configurations. The beamline is equipped with a single-axis goniometer, neutron-sensitive cylindrical image plate detector and room temperature and cryogenic sample environments. This article describes the beamline components, the diffractometer and the data collection and data analysis protocols that are used, and outlines the protein deuteration, crystallization and conventional crystallography capabilities that are available to users at ORNL's neutron facilities. We also present examples of the scientific questions being addressed at this beamline and highlight important findings in enzyme chemistry that have been made possible by IMAGINE.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1606037

Journal Information:: Methods in Enzymology, Journal Name: Methods in Enzymology Journal Issue: 1 Vol. 634; ISSN 0076-6879

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Title: IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor

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