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Title: Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers

Abstract

The structure and dynamics of lipid membranes in the presence of extracellular macromolecules are critical for cell membrane functions and many pharmaceutical applications. The pathogen virulence-suppressing end-phosphorylated polyethylene glycol (PEG) triblock copolymer (Pi-ABAPEG) markedly changes the interactions with lipid vesicle membranes and prevents PEG-induced vesicle phase separation in contrast to the unphosphorylated copolymer (ABAPEG). Pi-ABAPEG weakly absorbs on the surface of lipid vesicle membranes and slightly changes the structure of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) unilamellar vesicles at 37 °C, as evidenced by small angle neutron scattering. X-ray reflectivity measurements confirm the weak adsorption of Pi-ABAPEG on DMPC monolayer, resulting in a more compact DMPC monolayer structure. Neutron spin-echo results show that the adsorption of Pi-ABAPEG on DMPC vesicle membranes increases the membrane bending modulus κ.

Authors:
ORCiD logo [1];  [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [3]; ORCiD logo [4];  [5];  [6]; ORCiD logo [7]; ORCiD logo [1]; ORCiD logo [1]
  1. Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States). Materials Science Division
  2. National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States); Indiana Univ., Bloomington, IN (United States)
  3. Univ. of Chicago, IL (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science (CNMS)
  5. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
  6. National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States). Center for Neutron Research
  7. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
US Department of Commerce; National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Materials Sciences & Engineering Division; USDOE
OSTI Identifier:
1605989
Alternate Identifier(s):
OSTI ID: 1579770; OSTI ID: 1777825
Grant/Contract Number:  
AC02-06CH11357; AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Soft Matter
Additional Journal Information:
Journal Volume: 16; Journal Issue: 4; Journal ID: ISSN 1744-683X
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Yu, Jing, Mao, Jun, Nagao, Michihiro, Bu, Wei, Lin, Binhua, Hong, Kunlun, Jiang, Zhang, Liu, Yun, Qian, Shuo, Tirrell, Matthew, and Chen, Wei. Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers. United States: N. p., 2020. Web. https://doi.org/10.1039/c9sm01642b.
Yu, Jing, Mao, Jun, Nagao, Michihiro, Bu, Wei, Lin, Binhua, Hong, Kunlun, Jiang, Zhang, Liu, Yun, Qian, Shuo, Tirrell, Matthew, & Chen, Wei. Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers. United States. https://doi.org/10.1039/c9sm01642b
Yu, Jing, Mao, Jun, Nagao, Michihiro, Bu, Wei, Lin, Binhua, Hong, Kunlun, Jiang, Zhang, Liu, Yun, Qian, Shuo, Tirrell, Matthew, and Chen, Wei. Wed . "Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers". United States. https://doi.org/10.1039/c9sm01642b. https://www.osti.gov/servlets/purl/1605989.
@article{osti_1605989,
title = {Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers},
author = {Yu, Jing and Mao, Jun and Nagao, Michihiro and Bu, Wei and Lin, Binhua and Hong, Kunlun and Jiang, Zhang and Liu, Yun and Qian, Shuo and Tirrell, Matthew and Chen, Wei},
abstractNote = {The structure and dynamics of lipid membranes in the presence of extracellular macromolecules are critical for cell membrane functions and many pharmaceutical applications. The pathogen virulence-suppressing end-phosphorylated polyethylene glycol (PEG) triblock copolymer (Pi-ABAPEG) markedly changes the interactions with lipid vesicle membranes and prevents PEG-induced vesicle phase separation in contrast to the unphosphorylated copolymer (ABAPEG). Pi-ABAPEG weakly absorbs on the surface of lipid vesicle membranes and slightly changes the structure of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) unilamellar vesicles at 37 °C, as evidenced by small angle neutron scattering. X-ray reflectivity measurements confirm the weak adsorption of Pi-ABAPEG on DMPC monolayer, resulting in a more compact DMPC monolayer structure. Neutron spin-echo results show that the adsorption of Pi-ABAPEG on DMPC vesicle membranes increases the membrane bending modulus κ.},
doi = {10.1039/c9sm01642b},
journal = {Soft Matter},
number = 4,
volume = 16,
place = {United States},
year = {2020},
month = {1}
}

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