Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers
Abstract
The structure and dynamics of lipid membranes in the presence of extracellular macromolecules are critical for cell membrane functions and many pharmaceutical applications. The pathogen virulence-suppressing end-phosphorylated polyethylene glycol (PEG) triblock copolymer (Pi-ABAPEG) markedly changes the interactions with lipid vesicle membranes and prevents PEG-induced vesicle phase separation in contrast to the unphosphorylated copolymer (ABAPEG). Pi-ABAPEG weakly absorbs on the surface of lipid vesicle membranes and slightly changes the structure of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) unilamellar vesicles at 37 °C, as evidenced by small angle neutron scattering. X-ray reflectivity measurements confirm the weak adsorption of Pi-ABAPEG on DMPC monolayer, resulting in a more compact DMPC monolayer structure. Neutron spin-echo results show that the adsorption of Pi-ABAPEG on DMPC vesicle membranes increases the membrane bending modulus κ.
- Authors:
-
[1];
[2];
[3];
[3];
[4];
[7];
[1];
[1]
- Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States). Materials Science Division
- National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States); Indiana Univ., Bloomington, IN (United States)
- Univ. of Chicago, IL (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science (CNMS)
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States). Center for Neutron Research
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- US Department of Commerce; National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Materials Sciences & Engineering Division; USDOE
- OSTI Identifier:
- 1605989
- Alternate Identifier(s):
- OSTI ID: 1579770; OSTI ID: 1777825
- Grant/Contract Number:
- AC02-06CH11357; AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Soft Matter
- Additional Journal Information:
- Journal Volume: 16; Journal Issue: 4; Journal ID: ISSN 1744-683X
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Yu, Jing, Mao, Jun, Nagao, Michihiro, Bu, Wei, Lin, Binhua, Hong, Kunlun, Jiang, Zhang, Liu, Yun, Qian, Shuo, Tirrell, Matthew, and Chen, Wei. Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers. United States: N. p., 2020.
Web. doi:10.1039/c9sm01642b.
Yu, Jing, Mao, Jun, Nagao, Michihiro, Bu, Wei, Lin, Binhua, Hong, Kunlun, Jiang, Zhang, Liu, Yun, Qian, Shuo, Tirrell, Matthew, & Chen, Wei. Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers. United States. https://doi.org/10.1039/c9sm01642b
Yu, Jing, Mao, Jun, Nagao, Michihiro, Bu, Wei, Lin, Binhua, Hong, Kunlun, Jiang, Zhang, Liu, Yun, Qian, Shuo, Tirrell, Matthew, and Chen, Wei. Wed .
"Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers". United States. https://doi.org/10.1039/c9sm01642b. https://www.osti.gov/servlets/purl/1605989.
@article{osti_1605989,
title = {Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers},
author = {Yu, Jing and Mao, Jun and Nagao, Michihiro and Bu, Wei and Lin, Binhua and Hong, Kunlun and Jiang, Zhang and Liu, Yun and Qian, Shuo and Tirrell, Matthew and Chen, Wei},
abstractNote = {The structure and dynamics of lipid membranes in the presence of extracellular macromolecules are critical for cell membrane functions and many pharmaceutical applications. The pathogen virulence-suppressing end-phosphorylated polyethylene glycol (PEG) triblock copolymer (Pi-ABAPEG) markedly changes the interactions with lipid vesicle membranes and prevents PEG-induced vesicle phase separation in contrast to the unphosphorylated copolymer (ABAPEG). Pi-ABAPEG weakly absorbs on the surface of lipid vesicle membranes and slightly changes the structure of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) unilamellar vesicles at 37 °C, as evidenced by small angle neutron scattering. X-ray reflectivity measurements confirm the weak adsorption of Pi-ABAPEG on DMPC monolayer, resulting in a more compact DMPC monolayer structure. Neutron spin-echo results show that the adsorption of Pi-ABAPEG on DMPC vesicle membranes increases the membrane bending modulus κ.},
doi = {10.1039/c9sm01642b},
journal = {Soft Matter},
number = 4,
volume = 16,
place = {United States},
year = {2020},
month = {1}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
A-769662 activates AMPK β 1 -containing complexes but induces glucose uptake through a PI3-kinase-dependent pathway in mouse skeletal muscle
journal, October 2009
- Treebak, Jonas T.; Birk, Jesper B.; Hansen, Bo F.
- American Journal of Physiology-Cell Physiology, Vol. 297, Issue 4
Lipid Bilayers and Membrane Dynamics: Insight into Thickness Fluctuations
journal, July 2012
- Woodka, Andrea C.; Butler, Paul D.; Porcar, Lionel
- Physical Review Letters, Vol. 109, Issue 5
DAVE: A Comprehensive Software Suite for the Reduction, Visualization, and Analysis of Low Energy Neutron Spectroscopic Data
journal, November 2009
- Azuah, Richard Tumanjong; Kneller, Larry R.; Qiu, Yiming
- Journal of Research of the National Institute of Standards and Technology, Vol. 114, Issue 6
X-ray Studies of Interfacial Strontium–Extractant Complexes in a Model Solvent Extraction System
journal, October 2014
- Bu, Wei; Mihaylov, Miroslav; Amoanu, Daniel
- The Journal of Physical Chemistry B, Vol. 118, Issue 43
Tailoring Biomimetic Phosphorylcholine-Containing Block Copolymers as Membrane-Targeting Cellular Rescue Agents
journal, August 2019
- Wang, Jia-Yu; Chen, Wei; Nagao, Michihiro
- Biomacromolecules, Vol. 20, Issue 9
Osmotic and Curvature Stress Affect PEG-Induced Fusion of Lipid Vesicles but Not Mixing of Their Lipids
journal, April 2002
- Malinin, Vladimir S.; Frederik, Peter; Lentz, Barry R.
- Biophysical Journal, Vol. 82, Issue 4
Probing Elastic and Viscous Properties of Phospholipid Bilayers Using Neutron Spin Echo Spectroscopy
journal, September 2017
- Nagao, Michihiro; Kelley, Elizabeth G.; Ashkar, Rana
- The Journal of Physical Chemistry Letters, Vol. 8, Issue 19
Nature of Interactions between PEO-PPO-PEO Triblock Copolymers and Lipid Membranes: (I) Effect of Polymer Hydrophobicity on Its Ability to Protect Liposomes from Peroxidation
journal, August 2012
- Wang, Jia-Yu; Marks, Jeremy; Lee, Ka Yee C.
- Biomacromolecules, Vol. 13, Issue 9
Atomic Force Microscopic Analysis of the Effect of Lipid Composition on Liposome Membrane Rigidity
journal, June 2016
- Takechi-Haraya, Yuki; Sakai-Kato, Kumiko; Abe, Yasuhiro
- Langmuir, Vol. 32, Issue 24
Scattering Functions of Semiflexible Polymers with and without Excluded Volume Effects
journal, January 1996
- Pedersen, Jan Skov; Schurtenberger, Peter
- Macromolecules, Vol. 29, Issue 23
Lateral phase separation in tense membranes
journal, January 2011
- Hamada, Tsutomu; Kishimoto, Yuko; Nagasaki, Takeshi
- Soft Matter, Vol. 7, Issue 19
Deformation of giant lipid vesicles by electric fields
journal, December 1991
- Kummrow, M.; Helfrich, W.
- Physical Review A, Vol. 44, Issue 12
Method for obtaining structure and interactions from oriented lipid bilayers
journal, December 2000
- Lyatskaya, Yulia; Liu, Yufeng; Tristram-Nagle, Stephanie
- Physical Review E, Vol. 63, Issue 1
Oscillatory phase separation in giant lipid vesicles induced by transmembrane osmotic differentials
journal, October 2014
- Oglęcka, Kamila; Rangamani, Padmini; Liedberg, Bo
- eLife, Vol. 3
Phosphate-Containing Polyethylene Glycol Polymers Prevent Lethal Sepsis by Multidrug-Resistant Pathogens
journal, November 2013
- Zaborin, Alexander; Defazio, Jennifer R.; Kade, Matthew
- Antimicrobial Agents and Chemotherapy, Vol. 58, Issue 2
Dynamics in the plasma membrane: how to combine fluidity and order
journal, June 2006
- Marguet, Didier; Lenne, Pierre-François; Rigneault, Hervé
- The EMBO Journal, Vol. 25, Issue 15
Lipid-polyethylene glycol interactions: II. Formation of defects in bilayers
journal, February 1981
- Boni, L. T.; Stewart, T. P.; Alderfer, J. L.
- The Journal of Membrane Biology, Vol. 62, Issue 1-2
Universal Behavior of Membranes with Sterols
journal, March 2006
- Henriksen, J.; Rowat, A. C.; Brief, E.
- Biophysical Journal, Vol. 90, Issue 5
Packing Stress Relaxation in Polymer−Lipid Monolayers at the Air−Water Interface: An X-ray Grazing-Incidence Diffraction and Reflectivity Study
journal, August 1999
- Kuhl, T. L.; Majewski, J.; Howes, P. B.
- Journal of the American Chemical Society, Vol. 121, Issue 33
De Novo Synthesis of Phosphorylated Triblock Copolymers with Pathogen Virulence-Suppressing Properties That Prevent Infection-Related Mortality
journal, June 2017
- Mao, Jun; Zaborin, Alexander; Poroyko, Valeriy
- ACS Biomaterials Science & Engineering, Vol. 3, Issue 9
Membrane softening by nonsteroidal anti-inflammatory drugs investigated by neutron spin echo
journal, January 2019
- Sharma, V. K.; Nagao, M.; Rai, D. K.
- Physical Chemistry Chemical Physics, Vol. 21, Issue 36
Temperature Dependence of Structure, Bending Rigidity, and Bilayer Interactions of Dioleoylphosphatidylcholine Bilayers
journal, January 2008
- Pan, Jianjun; Tristram-Nagle, Stephanie; Kučerka, Norbert
- Biophysical Journal, Vol. 94, Issue 1
Drag of a Solid Particle Trapped in a Thin Film or at an Interface: Influence of Surface Viscosity and Elasticity
journal, June 2000
- Dimova, Rumiana; Danov, Krassimir; Pouligny, Bernard
- Journal of Colloid and Interface Science, Vol. 226, Issue 1
Structure of Fully Hydrated Fluid Phase Lipid Bilayers with Monounsaturated Chains
journal, January 2006
- Kučerka, Norbert; Tristram-Nagle, Stephanie; Nagle, John F.
- Journal of Membrane Biology, Vol. 208, Issue 3
The helix-to-sheet transition of an HIV-1 fusion peptide derivative changes the mechanical properties of lipid bilayer membranes
journal, March 2019
- Heller, William T.; Zolnierczuk, Piotr A.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1861, Issue 3
Thermal Fluctuation and Elasticity of Lipid Vesicles Interacting with Pore-Forming Peptides
journal, July 2010
- Lee, Ji-Hwan; Choi, Sung-Min; Doe, Changwoo
- Physical Review Letters, Vol. 105, Issue 3
Reduction and analysis of SANS and USANS data using IGOR Pro
journal, November 2006
- Kline, Steven R.
- Journal of Applied Crystallography, Vol. 39, Issue 6, p. 895-900
Undulations and Dynamic Structure Factor of Membranes
journal, December 1996
- Zilman, A. G.; Granek, R.
- Physical Review Letters, Vol. 77, Issue 23
Recent developments in the field of bending rigidity measurements on membranes
journal, June 2014
- Dimova, Rumiana
- Advances in Colloid and Interface Science, Vol. 208
Generalized skew-symmetric interfacial probability distribution in reflectivity and small-angle scattering analysis
journal, November 2017
- Jiang, Zhang; Chen, Wei
- Journal of Applied Crystallography, Vol. 50, Issue 6
Membrane dynamics and structure factor
journal, November 2002
- Zilman, Anton G.; Granek, Rony
- Chemical Physics, Vol. 284, Issue 1-2
Resolving the structural interactions between antimicrobial peptides and lipid membranes using small-angle scattering methods: the case of indolicidin
journal, January 2018
- Nielsen, Josefine Eilsø; Bjørnestad, Victoria Ariel; Lund, Reidar
- Soft Matter, Vol. 14, Issue 43
Effect of Line Tension on the Lateral Organization of Lipid Membranes
journal, September 2007
- García-Sáez, Ana J.; Chiantia, Salvatore; Schwille, Petra
- Journal of Biological Chemistry, Vol. 282, Issue 46
Determination of Biomembrane Bending Moduli in Fully Atomistic Simulations
journal, September 2014
- Levine, Zachary A.; Venable, Richard M.; Watson, Max C.
- Journal of the American Chemical Society, Vol. 136, Issue 39
Bio-SANS—A dedicated facility for neutron structural biology at Oak Ridge National Laboratory
journal, November 2006
- Lynn, G. W.; Heller, William; Urban, Volker
- Physica B: Condensed Matter, Vol. 385-386
How Accurate Is Poisson−Boltzmann Theory for Monovalent Ions near Highly Charged Interfaces?
journal, June 2006
- Bu, Wei; Vaknin, David; Travesset, Alex
- Langmuir, Vol. 22, Issue 13
Focusing cold neutrons with multiple biconcave lenses for small-angle neutron scattering
journal, June 2000
- Choi, S. -M.; Barker, J. G.; Glinka, C. J.
- Journal of Applied Crystallography, Vol. 33, Issue 3
Polymer-induced membrane fusion: potential mechanism and relation to cell fusion events
journal, September 1994
- Lentz, Barry R.
- Chemistry and Physics of Lipids, Vol. 73, Issue 1-2
Coarse-Grained Model for PEGylated Lipids: Effect of PEGylation on the Size and Shape of Self-Assembled Structures
journal, June 2011
- Lee, Hwankyu; Pastor, Richard W.
- The Journal of Physical Chemistry B, Vol. 115, Issue 24
Mimicry and Mechanism in Phospholipid Models of Membrane Fusion
journal, October 1986
- Rand, R. P.; Parsegian, V. A.
- Annual Review of Physiology, Vol. 48, Issue 1
DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin
journal, January 2018
- Sreij, Ramsia; Dargel, Carina; Geisler, Philippe
- Physical Chemistry Chemical Physics, Vol. 20, Issue 14
Nature of Interactions between PEO-PPO-PEO Triblock Copolymers and Lipid Membranes: (II) Role of Hydration Dynamics Revealed by Dynamic Nuclear Polarization
journal, August 2012
- Cheng, Chi-Yuan; Wang, Jia-Yu; Kausik, Ravinath
- Biomacromolecules, Vol. 13, Issue 9
Rupture of Lipid Vesicles by a Broad-Spectrum Antiviral Peptide: Influence of Vesicle Size
journal, November 2013
- Jackman, Joshua A.; Zan, Goh Haw; Zhdanov, Vladimir P.
- The Journal of Physical Chemistry B, Vol. 117, Issue 50
Membranes are more mosaic than fluid
journal, November 2005
- Engelman, Donald M.
- Nature, Vol. 438, Issue 7068
Interpreting Membrane Scattering Experiments at the Mesoscale: The Contribution of Dissipation within the Bilayer
journal, March 2010
- Watson, Max C.; Brown, Frank L. H.
- Biophysical Journal, Vol. 98, Issue 6
Vesicle fluctuation analysis of the effects of sterols on membrane bending rigidity
journal, June 2004
- Henriksen, Jonas; Rowat, Amy C.; Ipsen, John H.
- European Biophysics Journal, Vol. 33, Issue 8
Hydration or steric forces between amphiphilic surfaces?
journal, April 1990
- Israelachvili, Jacob N.; Wennerstroem, Haakan
- Langmuir, Vol. 6, Issue 4
Structure of fully hydrated fluid phase lipid bilayers with monounsaturated chains.
text, January 2018
- Kucerka, Norbert; Tristram-Nagle, Stephanie; Nagle, John F.
- Figshare
Temperature Dependence of Structure, Bending Rigidity, and Bilayer Interactions of Dioleoylphosphatidylcholine Bilayers
text, January 2008
- Pan, Jianjun; Tristram-Nagle, Stephanie; Kucerka, Norbert
- Carnegie Mellon University
Method for obtaining structure and interactions from oriented lipid bilayers
text, January 2000
- Lyatskaya, Yulia; Liu, Yufeng; Tristram-Nagle, Stephanie
- Carnegie Mellon University
Temperature Dependence of Structure, Bending Rigidity, and Bilayer Interactions of Dioleoylphosphatidylcholine Bilayers
text, January 2018
- Pan, Jianjun; Tristram-Nagle, Stephanie; Kucerka, Norbert
- Figshare
Osmotic and Curvature Stress Affect PEG-Induced Fusion of Lipid Vesicles but Not Mixing of Their Lipids
text, January 2002
- Peter, Frederik,; R., Lentz, Barry; S., Malinin, Vladimir
- The University of North Carolina at Chapel Hill University Libraries
Structure of fully hydrated fluid phase lipid bilayers with monounsaturated chains.
text, January 2018
- Kucerka, Norbert; Tristram-Nagle, Stephanie; Nagle, John F.
- Figshare