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Title: Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis

Abstract

Abstract Pennycress (Thlaspi arvense L.) accumulates oil up to 35% of the total seed biomass, and its overall fatty acid composition is suitable for aviation fuel. However, for this plant to become economically viable, its oil production needs to be improved. In vivo culture conditions that resemble the development of pennycress embryos in planta were developed based on the composition of the liquid endosperm. Then, substrate uptake rates and biomass accumulation were measured from cultured pennycress embryos, revealing a biosynthetic efficiency of 93%, which is one of the highest in comparison with other oilseeds to date. Additionally, the ratio of carbon in oil to CO2 indicated that non-conventional pathways are likely to be responsible for such a high carbon conversion efficiency. To identify the reactions enabling this phenomenon, parallel labeling experiments with 13C-labeled substrates were conducted in pennycress embryos. The main findings of these labeling experiments include: (i) the occurrence of the oxidative reactions of the pentose phosphate pathway in the cytosol; (ii) the reversibility of isocitrate dehydrogenase; (iii) the operation of the plastidic NADP-dependent malic enzyme; and (iv) the refixation of CO2 by Rubisco. These reactions are key providers of carbon and reductant for fatty acid synthesis and elongation.

Authors:
 [1];  [2]; ORCiD logo [3];  [4]
  1. Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, USA
  2. BioDiscovery Institute, University of North Texas, Denton, TX, USA
  3. BioDiscovery Institute, University of North Texas, Denton, TX, USA, Department of Biological Sciences, University of North Texas, Denton, TX, USA
  4. MPI of Molecular Plant Physiology, Germany
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1605939
Grant/Contract Number:  
[SC0019233]
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
[Journal Name: Journal of Experimental Botany]; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Tsogtbaatar, Enkhtuul, Cocuron, Jean-Christophe, Alonso, Ana Paula, and Lunn, ed., John. Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis. United Kingdom: N. p., 2020. Web. doi:10.1093/jxb/eraa060.
Tsogtbaatar, Enkhtuul, Cocuron, Jean-Christophe, Alonso, Ana Paula, & Lunn, ed., John. Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis. United Kingdom. doi:10.1093/jxb/eraa060.
Tsogtbaatar, Enkhtuul, Cocuron, Jean-Christophe, Alonso, Ana Paula, and Lunn, ed., John. Sat . "Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis". United Kingdom. doi:10.1093/jxb/eraa060.
@article{osti_1605939,
title = {Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis},
author = {Tsogtbaatar, Enkhtuul and Cocuron, Jean-Christophe and Alonso, Ana Paula and Lunn, ed., John},
abstractNote = {Abstract Pennycress (Thlaspi arvense L.) accumulates oil up to 35% of the total seed biomass, and its overall fatty acid composition is suitable for aviation fuel. However, for this plant to become economically viable, its oil production needs to be improved. In vivo culture conditions that resemble the development of pennycress embryos in planta were developed based on the composition of the liquid endosperm. Then, substrate uptake rates and biomass accumulation were measured from cultured pennycress embryos, revealing a biosynthetic efficiency of 93%, which is one of the highest in comparison with other oilseeds to date. Additionally, the ratio of carbon in oil to CO2 indicated that non-conventional pathways are likely to be responsible for such a high carbon conversion efficiency. To identify the reactions enabling this phenomenon, parallel labeling experiments with 13C-labeled substrates were conducted in pennycress embryos. The main findings of these labeling experiments include: (i) the occurrence of the oxidative reactions of the pentose phosphate pathway in the cytosol; (ii) the reversibility of isocitrate dehydrogenase; (iii) the operation of the plastidic NADP-dependent malic enzyme; and (iv) the refixation of CO2 by Rubisco. These reactions are key providers of carbon and reductant for fatty acid synthesis and elongation.},
doi = {10.1093/jxb/eraa060},
journal = {Journal of Experimental Botany},
number = ,
volume = ,
place = {United Kingdom},
year = {2020},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1093/jxb/eraa060

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