Ecosystem Controls on Methylmercury Production by Periphyton Biofilms in a Contaminated Stream: Implications for Predictive Modeling
Abstract
Periphyton biofilms produce a substantial fraction of the overall monomethylmercury (MMHg) flux in East Fork Poplar Creek, an industrially contaminated, freshwater creek in Oak Ridge, Tennessee. We examined periphyton MMHg production across seasons, locations, and light conditions using mercury stable isotopes. Methylation and demethylation rate potentials ($$k_{m, trans av}$$ and $$k_{d, trans av}$$, respectively) were calculated using a transient availability kinetic model. Light exposure and season were significant predictors of $$k_{m, trans av}$$, with greater values in full light exposure and in the summer. Season, light exposure, and location were significant predictors of $$k_{d, trans av}$$, which was highest in dark conditions, in the spring, and at the upstream location. Light exposure was the controlling factor for net MMHg production, with positive production for periphyton grown under full light exposure and net demethylation for periphyton grown in the dark. Ambient MMHg and km, trans av were significantly correlated. Transient availability rate potentials were 15 times higher for km and 9 times higher for kd compared to full availability rate potentials ($$k_{m, full av}$$ and $$k_{d, full av}$$) calculated at 1 d. No significant model for the prediction of $$k_{m, full av}$$ and $$k_{d, full av}$$ could be constructed using light, season, and location. In addition, there were no significant differences among treatments for the full availability $$k_{m, full av}$$ and $$k_{d, full av}$$, or net MMHg calculated using the full availability rate potentials. km, full av was not correlated with ambient MMHg concentrations. The present results underscore the importance of applying transient availability kinetics to MMHg production data when estimating MMHg production potential and flux.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1605912
- Report Number(s):
- PNNL-SA-147041
Journal ID: ISSN 0730-7268
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Toxicology and Chemistry
- Additional Journal Information:
- Journal Volume: 38; Journal Issue: 11; Journal ID: ISSN 0730-7268
- Publisher:
- SETAC
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; Periphyton; Methylmercury; Kinetics
Citation Formats
Schwartz, Grace E., Olsen, Todd A., Muller, Katherine A., and Brooks, Scott C. Ecosystem Controls on Methylmercury Production by Periphyton Biofilms in a Contaminated Stream: Implications for Predictive Modeling. United States: N. p., 2019.
Web. doi:10.1002/etc.4551.
Schwartz, Grace E., Olsen, Todd A., Muller, Katherine A., & Brooks, Scott C. Ecosystem Controls on Methylmercury Production by Periphyton Biofilms in a Contaminated Stream: Implications for Predictive Modeling. United States. https://doi.org/10.1002/etc.4551
Schwartz, Grace E., Olsen, Todd A., Muller, Katherine A., and Brooks, Scott C. Wed .
"Ecosystem Controls on Methylmercury Production by Periphyton Biofilms in a Contaminated Stream: Implications for Predictive Modeling". United States. https://doi.org/10.1002/etc.4551. https://www.osti.gov/servlets/purl/1605912.
@article{osti_1605912,
title = {Ecosystem Controls on Methylmercury Production by Periphyton Biofilms in a Contaminated Stream: Implications for Predictive Modeling},
author = {Schwartz, Grace E. and Olsen, Todd A. and Muller, Katherine A. and Brooks, Scott C.},
abstractNote = {Periphyton biofilms produce a substantial fraction of the overall monomethylmercury (MMHg) flux in East Fork Poplar Creek, an industrially contaminated, freshwater creek in Oak Ridge, Tennessee. We examined periphyton MMHg production across seasons, locations, and light conditions using mercury stable isotopes. Methylation and demethylation rate potentials ($k_{m, trans av}$ and $k_{d, trans av}$, respectively) were calculated using a transient availability kinetic model. Light exposure and season were significant predictors of $k_{m, trans av}$, with greater values in full light exposure and in the summer. Season, light exposure, and location were significant predictors of $k_{d, trans av}$, which was highest in dark conditions, in the spring, and at the upstream location. Light exposure was the controlling factor for net MMHg production, with positive production for periphyton grown under full light exposure and net demethylation for periphyton grown in the dark. Ambient MMHg and km, trans av were significantly correlated. Transient availability rate potentials were 15 times higher for km and 9 times higher for kd compared to full availability rate potentials ($k_{m, full av}$ and $k_{d, full av}$) calculated at 1 d. No significant model for the prediction of $k_{m, full av}$ and $k_{d, full av}$ could be constructed using light, season, and location. In addition, there were no significant differences among treatments for the full availability $k_{m, full av}$ and $k_{d, full av}$, or net MMHg calculated using the full availability rate potentials. km, full av was not correlated with ambient MMHg concentrations. The present results underscore the importance of applying transient availability kinetics to MMHg production data when estimating MMHg production potential and flux.},
doi = {10.1002/etc.4551},
journal = {Environmental Toxicology and Chemistry},
number = 11,
volume = 38,
place = {United States},
year = {2019},
month = {7}
}
Figures / Tables:

Works referenced in this record:
Methanogens: Principal Methylators of Mercury in Lake Periphyton
journal, September 2011
- Hamelin, Stéphanie; Amyot, Marc; Barkay, Tamar
- Environmental Science & Technology, Vol. 45, Issue 18
A little bit of light goes a long way: the role of phototrophs on mercury cycling
journal, January 2014
- Grégoire, Daniel S.; Poulain, A. J.
- Metallomics, Vol. 6, Issue 3
Importance of sulfate reducing bacteria in mercury methylation and demethylation in periphyton from Bolivian Amazon region
journal, February 2011
- Achá, Darío; Hintelmann, Holger; Yee, Janet
- Chemosphere, Vol. 82, Issue 6
The Toxicology of Mercury — Current Exposures and Clinical Manifestations
journal, October 2003
- Clarkson, Thomas W.; Magos, Laszlo; Myers, Gary J.
- New England Journal of Medicine, Vol. 349, Issue 18
Bioaccumulation of mercury in benthic communities of a river ecosystem affected by mercury mining
journal, May 2007
- Žižek, Suzana; Horvat, Milena; Gibičar, Darija
- Science of The Total Environment, Vol. 377, Issue 2-3
Artifact formation of methyl mercury during aqueous distillation and alternative techniques for the extraction of methyl mercury from environmental samples
journal, June 1997
- Bloom, N. S.; Colman, John A.; Barber, Lee
- Fresenius' Journal of Analytical Chemistry, Vol. 358, Issue 3
Kinetics of mercury accumulation by freshwater biofilms
journal, January 2017
- Dranguet, Perrine; Slaveykova, Vera I.; Le Faucheur, Séverine
- Environmental Chemistry, Vol. 14, Issue 7
Bacterial periphytic communities related to mercury methylation within aquatic plant roots from a temperate freshwater lake (South-Western France)
journal, June 2017
- Gentès, Sophie; Taupiac, Julie; Colin, Yannick
- Environmental Science and Pollution Research, Vol. 24, Issue 23
Mercury methylation rates of biofilm and plankton microorganisms from a hydroelectric reservoir in French Guiana
journal, February 2010
- Huguet, L.; Castelle, S.; Schäfer, J.
- Science of The Total Environment, Vol. 408, Issue 6
Calculations of double spike isotope dilution results revisited
journal, January 2006
- Meija, Juris; Yang, Lu; Caruso, Joseph A.
- Journal of Analytical Atomic Spectrometry, Vol. 21, Issue 11
Molecular composition of organic matter controls methylmercury formation in boreal lakes
journal, February 2017
- Bravo, Andrea G.; Bouchet, Sylvain; Tolu, Julie
- Nature Communications, Vol. 8, Issue 1
Mercury methylation in periphyton of the Florida Everglades
journal, October 1999
- Cleckner, Lisa B.; Gilmour, Cynthia C.; Hurley, James P.
- Limnology and Oceanography, Vol. 44, Issue 7
High methylation rates of mercury bound to cysteine by Geobacter sulfurreducens
journal, January 2009
- Schaefer, Jeffra K.; Morel, François M. M.
- Nature Geoscience, Vol. 2, Issue 2
Periphyton Biofilms Influence Net Methylmercury Production in an Industrially Contaminated System
journal, September 2016
- Olsen, Todd A.; Brandt, Craig C.; Brooks, Scott C.
- Environmental Science & Technology, Vol. 50, Issue 20
Stoichiometry and kinetics of mercury uptake by photosynthetic bacteria
journal, March 2017
- Kis, Mariann; Sipka, Gábor; Maróti, Péter
- Photosynthesis Research, Vol. 132, Issue 2
Dissolved Organic Matter Enhances Microbial Mercury Methylation Under Sulfidic Conditions
journal, February 2012
- Graham, Andrew M.; Aiken, George R.; Gilmour, Cynthia C.
- Environmental Science & Technology, Vol. 46, Issue 5
Molecular Characterization of Mercury Binding Ligands Released by Freshwater Algae Grown at Three Photoperiods
journal, January 2019
- Mangal, Vaughn; Phung, Ta; Nguyen, Thien Q.
- Frontiers in Environmental Science, Vol. 6
Mercury Methylation in the Epilithon of Boreal Shield Aquatic Ecosystems
journal, March 2006
- Desrosiers, Mélanie; Planas, Dolors; Mucci, Alfonso
- Environmental Science & Technology, Vol. 40, Issue 5
Thiols in wetland interstitial waters and their role in mercury and methylmercury speciation
journal, November 2004
- Zhang, Jinzhong; Wang, Feiyue; House, James D.
- Limnology and Oceanography, Vol. 49, Issue 6
Mercury Methylation by Novel Microorganisms from New Environments
journal, September 2013
- Gilmour, Cynthia C.; Podar, Mircea; Bullock, Allyson L.
- Environmental Science & Technology, Vol. 47, Issue 20
Relationship between Extracellular Low-Molecular-Weight Thiols and Mercury Species in Natural Lake Periphytic Biofilms
journal, June 2015
- Leclerc, Maxime; Planas, Dolors; Amyot, Marc
- Environmental Science & Technology, Vol. 49, Issue 13
High-yield synthesis of milligram amounts of isotopically enriched methylmercury(CH3198HgCl)
journal, January 2004
- Bancon-Montigny, Chrystelle; Yang, Lu; Sturgeon, Ralph E.
- Applied Organometallic Chemistry, Vol. 18, Issue 2
Factors controlling the abiotic photo-degradation of monomethylmercury in surface waters
journal, May 2012
- Black, Frank J.; Poulin, Brett A.; Flegal, A. Russell
- Geochimica et Cosmochimica Acta, Vol. 84
The ecology and biogeochemistry of stream biofilms
journal, March 2016
- Battin, Tom J.; Besemer, Katharina; Bengtsson, Mia M.
- Nature Reviews Microbiology, Vol. 14, Issue 4
Seasonal and flow-driven dynamics of particulate and dissolved mercury and methylmercury in a stream impacted by an industrial mercury source: Hg and MeHg dynamics in an industrially contaminated stream
journal, March 2016
- Riscassi, Ami; Miller, Carrie; Brooks, Scott
- Environmental Toxicology and Chemistry, Vol. 35, Issue 6
Microelectrode studies of the photosynthesis and O 2 , H 2 S, and pH profiles of a microbial mat1: O 2 , H 2 S, and pH in microbial mat
journal, November 1983
- Revsbech, Niels Peter; Jorgensen, Bo Barker; Blackburn, T. Henry
- Limnology and Oceanography, Vol. 28, Issue 6
Distribution and biogeochemical controls on net methylmercury production in Penobscot River marshes and sediment
journal, November 2018
- Gilmour, Cynthia; Bell, James Tyler; Soren, Ally Bullock
- Science of The Total Environment, Vol. 640-641
16S rRNA assessment of the influence of shading on early-successional biofilms in experimental streams
journal, October 2015
- Lehmann, Katja; Singer, Andrew; Bowes, Michael J.
- FEMS Microbiology Ecology, Vol. 91, Issue 12
Mercury methylation and hydrogen sulfide production among unexpected strains isolated from periphyton of two macrophytes of the Amazon
journal, March 2012
- Achá, Dario; Pabón, Cecilia A.; Hintelmann, Holger
- FEMS Microbiology Ecology, Vol. 80, Issue 3
Oligotrophic wetland sediments susceptible to shifts in microbiomes and mercury cycling with dissolved organic matter addition
journal, January 2018
- Graham, Emily B.; Gabor, Rachel S.; Schooler, Shon
- PeerJ, Vol. 6
Active transport, substrate specificity, and methylation of Hg(II) in anaerobic bacteria
journal, May 2011
- Schaefer, J. K.; Rocks, S. S.; Zheng, W.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 21
Effect of Dissolved Organic Matter Source and Character on Microbial Hg Methylation in Hg–S–DOM Solutions
journal, May 2013
- Graham, Andrew M.; Aiken, George R.; Gilmour, Cynthia C.
- Environmental Science & Technology, Vol. 47, Issue 11
Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic Environment: A Critical Review
journal, February 2013
- Hsu-Kim, Heileen; Kucharzyk, Katarzyna H.; Zhang, Tong
- Environmental Science & Technology, Vol. 47, Issue 6
Aerobic and Anaerobic Bacterial Mercury Uptake is Driven by Algal Organic Matter Composition and Molecular Weight
journal, November 2018
- Mangal, Vaughn; Stenzler, Benjamin R.; Poulain, Alexandre J.
- Environmental Science & Technology, Vol. 53, Issue 1
Relative contributions of mercury bioavailability and microbial growth rate on net methylmercury production by anaerobic mixed cultures
journal, January 2015
- Kucharzyk, Katarzyna H.; Deshusses, Marc A.; Porter, Kaitlyn A.
- Environmental Science: Processes & Impacts, Vol. 17, Issue 9
Global prevalence and distribution of genes and microorganisms involved in mercury methylation
journal, October 2015
- Podar, Mircea; Gilmour, Cynthia C.; Brandt, Craig C.
- Science Advances, Vol. 1, Issue 9
Mercury methylation in macrophytes, periphyton, and water - comparative studies with stable and radio-mercury additions
journal, November 2002
- J., Mauro; J., Guimarães; H., Hintelmann
- Analytical and Bioanalytical Chemistry, Vol. 374, Issue 6
Kinetics of Methylmercury Production Revisited
journal, January 2018
- Olsen, Todd A.; Muller, Katherine A.; Painter, Scott L.
- Environmental Science & Technology, Vol. 52, Issue 4
History of mercury use and environmental contamination at the Oak Ridge Y-12 Plant
journal, January 2011
- Brooks, Scott C.; Southworth, George R.
- Environmental Pollution, Vol. 159, Issue 1
Mercury bioavailability, transformations, and effects on freshwater biofilms: Hg bioavailability to biofilms
journal, September 2017
- Dranguet, Perrine; Le Faucheur, Séverine; Slaveykova, Vera I.
- Environmental Toxicology and Chemistry, Vol. 36, Issue 12
Influence of light and temperature on the development and denitrification potential of periphytic biofilms
journal, February 2018
- Zhao, Yanhui; Xiong, Xiong; Wu, Chenxi
- Science of The Total Environment, Vol. 613-614
Figures / Tables found in this record: