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Title: Direct SERDS sensing of molecular biomarkers in plants under field conditions

Abstract

Molecular biomarkers such as microRNAs (miRNAs) play important roles in regulating various developmental processes in plants. Understanding these pathways will help bioengineer designing organisms for efficient biomass accumulation. Current methods for RNA analysis require sample extraction and multi-step sample analysis, hindering work in field studies. Recent work in the incorporation of nanomaterials for plant bioengineering research is leading the way of an agri-tech revolution. As an example, surface-enhanced Raman scattering (SERS)–based sensors can be used to monitor RNA in vivo. However, the use of SERS in the field has been limited due to issues with observing Raman signal over complex background. To this end, shifted-excitation Raman difference spectroscopy (SERDS) offers an effective solution to extract the SERS signal from high background based on a physical approach. In this manuscript, we report the first application of SERDS on SERS sensors. We investigated this technique on SERS sensor developed for the detection of a microRNA biomarker, miR858. We tested the technique on in vitro samples and validated the technique by detecting the presence of exogenous miR858 in plants directly under ambient light in a growth chamber. The possibility of moving the detection of nucleic acid targets outside the constraints of laboratory settingmore » enables numerous important bioengineering applications. Finally, such applications can revolutionize biofuel production and agri-tech through the use of nanotechnology-based monitoring of plant growth, plant health, and exposure to pollution and pathogens.« less

Authors:
 [1];  [1];  [2];  [2];  [1]
  1. Duke Univ., Durham, NC (United States)
  2. Leibniz-Inst. für Höchstfrequenztechnik, Berlin (Germany)
Publication Date:
Research Org.:
Duke Univ., Durham, NC (United States)
Sponsoring Org.:
USDOE Office of Science (SC); Federal Ministry of Education and Research (BMBF)
OSTI Identifier:
1605338
Grant/Contract Number:  
SC0019393; 031A564C
Resource Type:
Accepted Manuscript
Journal Name:
Analytical and Bioanalytical Chemistry
Additional Journal Information:
Journal Volume: 412; Journal ID: ISSN 1618-2642
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 09 BIOMASS FUELS; surface-enhanced Raman scattering; shifted-excitation difference Raman spectroscopy; sensing; plants; miRNA; field conditions

Citation Formats

Strobbia, Pietro, Odion, Ren A., Maiwald, Martin, Sumpf, Bernd, and Vo-Dinh, Tuan. Direct SERDS sensing of molecular biomarkers in plants under field conditions. United States: N. p., 2020. Web. doi:10.1007/s00216-020-02544-5.
Strobbia, Pietro, Odion, Ren A., Maiwald, Martin, Sumpf, Bernd, & Vo-Dinh, Tuan. Direct SERDS sensing of molecular biomarkers in plants under field conditions. United States. doi:https://doi.org/10.1007/s00216-020-02544-5
Strobbia, Pietro, Odion, Ren A., Maiwald, Martin, Sumpf, Bernd, and Vo-Dinh, Tuan. Mon . "Direct SERDS sensing of molecular biomarkers in plants under field conditions". United States. doi:https://doi.org/10.1007/s00216-020-02544-5. https://www.osti.gov/servlets/purl/1605338.
@article{osti_1605338,
title = {Direct SERDS sensing of molecular biomarkers in plants under field conditions},
author = {Strobbia, Pietro and Odion, Ren A. and Maiwald, Martin and Sumpf, Bernd and Vo-Dinh, Tuan},
abstractNote = {Molecular biomarkers such as microRNAs (miRNAs) play important roles in regulating various developmental processes in plants. Understanding these pathways will help bioengineer designing organisms for efficient biomass accumulation. Current methods for RNA analysis require sample extraction and multi-step sample analysis, hindering work in field studies. Recent work in the incorporation of nanomaterials for plant bioengineering research is leading the way of an agri-tech revolution. As an example, surface-enhanced Raman scattering (SERS)–based sensors can be used to monitor RNA in vivo. However, the use of SERS in the field has been limited due to issues with observing Raman signal over complex background. To this end, shifted-excitation Raman difference spectroscopy (SERDS) offers an effective solution to extract the SERS signal from high background based on a physical approach. In this manuscript, we report the first application of SERDS on SERS sensors. We investigated this technique on SERS sensor developed for the detection of a microRNA biomarker, miR858. We tested the technique on in vitro samples and validated the technique by detecting the presence of exogenous miR858 in plants directly under ambient light in a growth chamber. The possibility of moving the detection of nucleic acid targets outside the constraints of laboratory setting enables numerous important bioengineering applications. Finally, such applications can revolutionize biofuel production and agri-tech through the use of nanotechnology-based monitoring of plant growth, plant health, and exposure to pollution and pathogens.},
doi = {10.1007/s00216-020-02544-5},
journal = {Analytical and Bioanalytical Chemistry},
number = ,
volume = 412,
place = {United States},
year = {2020},
month = {3}
}

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