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Title: Time-resolved serial femtosecond crystallography at the European XFEL

Abstract

The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. In this paper, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.

Authors:
 [1];  [2];  [2];  [1];  [2]; ORCiD logo [3];  [4];  [4]; ORCiD logo [4];  [5];  [5]; ORCiD logo [4]; ORCiD logo [4];  [2]; ORCiD logo [3];  [3];  [2]; ORCiD logo [6]; ORCiD logo [3]; ORCiD logo [7] more »; ORCiD logo [2];  [2];  [3];  [2]; ORCiD logo [2];  [2];  [8];  [4]; ORCiD logo [2];  [3];  [2]; ORCiD logo [4];  [9]; ORCiD logo [2]; ORCiD logo [1];  [2];  [2]; ORCiD logo [9];  [1];  [2];  [2];  [4]; ORCiD logo [10]; ORCiD logo [3];  [4];  [3];  [11]; ORCiD logo [1] « less
  1. Univ. of Wisconsin, Milwaukee, WI (United States)
  2. European XFEL GmbH (EuXFEL), Schenefeld (Germany)
  3. Arizona State Univ., Tempe, AZ (United States)
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  6. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  7. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Hamburg Univ. of Technology (Germany)
  8. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg (Germany)
  9. European XFEL GmbH (EuXFEL), Schenefeld (Germany). Integrated Biology Infrastructure Life-Science Facility
  10. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg (Germany); Centre for Ultrafast Imaging, Hamburg (Germany)
  11. European XFEL GmbH (EuXFEL), Schenefeld (Germany); La Trobe Univ., Melbourne, VIC (Australia)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE; National Science Foundation (NSF); German Research Foundation (DFG); European Union’s 2020 Research and Innovation Program; European Research Council (ERC); Human Frontiers Science Program; Arizona State University; National Institutes of Health (NIH)
OSTI Identifier:
1605212
Grant/Contract Number:  
AC02-76SF00515; NSF-1231306; 637295; ERC-2013-SyG 609920; RGP0010 2017; 1565180; R01GM095583; R01GM117342
Resource Type:
Accepted Manuscript
Journal Name:
Nature Methods
Additional Journal Information:
Journal Volume: 17; Journal Issue: 1; Journal ID: ISSN 1548-7091
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Molecular biophysics; Nanocrystallography; Proteins

Citation Formats

Pandey, Suraj, Bean, Richard, Sato, Tokushi, Poudyal, Ishwor, Bielecki, Johan, Cruz Villarreal, Jorvani, Yefanov, Oleksandr, Mariani, Valerio, White, Thomas A., Kupitz, Christopher, Hunter, Mark, Abdellatif, Mohamed H., Bajt, Saša, Bondar, Valerii, Echelmeier, Austin, Doppler, Diandra, Emons, Moritz, Frank, Matthias, Fromme, Raimund, Gevorkov, Yaroslav, Giovanetti, Gabriele, Jiang, Man, Kim, Daihyun, Kim, Yoonhee, Kirkwood, Henry, Klimovskaia, Anna, Knoska, Juraj, Koua, Faisal H. M., Letrun, Romain, Lisova, Stella, Maia, Luis, Mazalova, Victoria, Meza, Domingo, Michelat, Thomas, Ourmazd, Abbas, Palmer, Guido, Ramilli, Marco, Schubert, Robin, Schwander, Peter, Silenzi, Alessandro, Sztuk-Dambietz, Jolanta, Tolstikova, Alexandra, Chapman, Henry N., Ros, Alexandra, Barty, Anton, Fromme, Petra, Mancuso, Adrian P., and Schmidt, Marius. Time-resolved serial femtosecond crystallography at the European XFEL. United States: N. p., 2019. Web. doi:10.1038/s41592-019-0628-z.
Pandey, Suraj, Bean, Richard, Sato, Tokushi, Poudyal, Ishwor, Bielecki, Johan, Cruz Villarreal, Jorvani, Yefanov, Oleksandr, Mariani, Valerio, White, Thomas A., Kupitz, Christopher, Hunter, Mark, Abdellatif, Mohamed H., Bajt, Saša, Bondar, Valerii, Echelmeier, Austin, Doppler, Diandra, Emons, Moritz, Frank, Matthias, Fromme, Raimund, Gevorkov, Yaroslav, Giovanetti, Gabriele, Jiang, Man, Kim, Daihyun, Kim, Yoonhee, Kirkwood, Henry, Klimovskaia, Anna, Knoska, Juraj, Koua, Faisal H. M., Letrun, Romain, Lisova, Stella, Maia, Luis, Mazalova, Victoria, Meza, Domingo, Michelat, Thomas, Ourmazd, Abbas, Palmer, Guido, Ramilli, Marco, Schubert, Robin, Schwander, Peter, Silenzi, Alessandro, Sztuk-Dambietz, Jolanta, Tolstikova, Alexandra, Chapman, Henry N., Ros, Alexandra, Barty, Anton, Fromme, Petra, Mancuso, Adrian P., & Schmidt, Marius. Time-resolved serial femtosecond crystallography at the European XFEL. United States. doi:https://doi.org/10.1038/s41592-019-0628-z
Pandey, Suraj, Bean, Richard, Sato, Tokushi, Poudyal, Ishwor, Bielecki, Johan, Cruz Villarreal, Jorvani, Yefanov, Oleksandr, Mariani, Valerio, White, Thomas A., Kupitz, Christopher, Hunter, Mark, Abdellatif, Mohamed H., Bajt, Saša, Bondar, Valerii, Echelmeier, Austin, Doppler, Diandra, Emons, Moritz, Frank, Matthias, Fromme, Raimund, Gevorkov, Yaroslav, Giovanetti, Gabriele, Jiang, Man, Kim, Daihyun, Kim, Yoonhee, Kirkwood, Henry, Klimovskaia, Anna, Knoska, Juraj, Koua, Faisal H. M., Letrun, Romain, Lisova, Stella, Maia, Luis, Mazalova, Victoria, Meza, Domingo, Michelat, Thomas, Ourmazd, Abbas, Palmer, Guido, Ramilli, Marco, Schubert, Robin, Schwander, Peter, Silenzi, Alessandro, Sztuk-Dambietz, Jolanta, Tolstikova, Alexandra, Chapman, Henry N., Ros, Alexandra, Barty, Anton, Fromme, Petra, Mancuso, Adrian P., and Schmidt, Marius. Mon . "Time-resolved serial femtosecond crystallography at the European XFEL". United States. doi:https://doi.org/10.1038/s41592-019-0628-z. https://www.osti.gov/servlets/purl/1605212.
@article{osti_1605212,
title = {Time-resolved serial femtosecond crystallography at the European XFEL},
author = {Pandey, Suraj and Bean, Richard and Sato, Tokushi and Poudyal, Ishwor and Bielecki, Johan and Cruz Villarreal, Jorvani and Yefanov, Oleksandr and Mariani, Valerio and White, Thomas A. and Kupitz, Christopher and Hunter, Mark and Abdellatif, Mohamed H. and Bajt, Saša and Bondar, Valerii and Echelmeier, Austin and Doppler, Diandra and Emons, Moritz and Frank, Matthias and Fromme, Raimund and Gevorkov, Yaroslav and Giovanetti, Gabriele and Jiang, Man and Kim, Daihyun and Kim, Yoonhee and Kirkwood, Henry and Klimovskaia, Anna and Knoska, Juraj and Koua, Faisal H. M. and Letrun, Romain and Lisova, Stella and Maia, Luis and Mazalova, Victoria and Meza, Domingo and Michelat, Thomas and Ourmazd, Abbas and Palmer, Guido and Ramilli, Marco and Schubert, Robin and Schwander, Peter and Silenzi, Alessandro and Sztuk-Dambietz, Jolanta and Tolstikova, Alexandra and Chapman, Henry N. and Ros, Alexandra and Barty, Anton and Fromme, Petra and Mancuso, Adrian P. and Schmidt, Marius},
abstractNote = {The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. In this paper, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.},
doi = {10.1038/s41592-019-0628-z},
journal = {Nature Methods},
number = 1,
volume = 17,
place = {United States},
year = {2019},
month = {11}
}

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    Works referencing / citing this record:

    Time-Resolved Serial Femtosecond Crystallography at the EuXFEL
    dataset, January 2019

    • Pandey, Suraj
    • Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); European XFEL
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